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DBCO-PEG4-NHS Ester
Cu-free click reaction, i.e., the strain-promoted alkyne-azide cycloaddition (SPAAC) is a bioorthogonal reaction utilizing a pair of reagents, cyclooctynes and azides that exclusively and efficiently react with each other while remain inert to naturally occurring functional groups such as hydroxy, amino and thiol groups. SPAAC enables labeling a wide variety of biomolecules without any auxiliary reagents in an aqueous and otherwise complex chemical environment through the formation of a stable triazole. Among the large number of known cyclooctynes, dibenzocyclooctyne (DBCO) compounds comprise a class of reagents that possesses reasonably fast kinetics and good stability in aqueous buffers. Within physiological temperature and pH ranges, the DBCO group will not react with thiol, amino or hydroxy groups that are naturally present in many biomolecules. DBCO-PEG4-NHS Ester is one of the most common reagents used for introducing DBCO group into a biomolecule via the modification of the amino groups.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DBCO-PEG4-NHS Ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 153.917 µL | 769.586 µL | 1.539 mL | 7.696 mL | 15.392 mL |
| 5 mM | 30.783 µL | 153.917 µL | 307.834 µL | 1.539 mL | 3.078 mL |
| 10 mM | 15.392 µL | 76.959 µL | 153.917 µL | 769.586 µL | 1.539 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Citations
View all 16 citations: Citation Explorer
The one-pot nonhydrolysis Staudinger reaction and Staudinger or SPAAC ligation
Authors: Cheng, L., Kang, X., Wang, D., Gao, Y., Yi, L., Xi, Z.
Journal: Org Biomol Chem (2019): 5675-5679
Authors: Cheng, L., Kang, X., Wang, D., Gao, Y., Yi, L., Xi, Z.
Journal: Org Biomol Chem (2019): 5675-5679
Highly reactive bis-cyclooctyne-modified diarylethene for SPAAC-mediated cross-linking
Authors: Strizhak, A. V., Sharma, K., Babii, O., Afonin, S., Ulrich, A. S., Komarov, I. V., Spring, D. R.
Journal: Org Biomol Chem (2018): 8559-8564
Authors: Strizhak, A. V., Sharma, K., Babii, O., Afonin, S., Ulrich, A. S., Komarov, I. V., Spring, D. R.
Journal: Org Biomol Chem (2018): 8559-8564
Optimization of Chemoenzymatic Mass Tagging by Strain-Promoted Cycloaddition (SPAAC) for the Determination of O-GlcNAc Stoichiometry by Western Blotting
Authors: Darabedian, N., Thompson, J. W., Chuh, K. N., Hsieh-Wilson, L. C., Pratt, M. R.
Journal: Biochemistry (2018): 5769-5774
Authors: Darabedian, N., Thompson, J. W., Chuh, K. N., Hsieh-Wilson, L. C., Pratt, M. R.
Journal: Biochemistry (2018): 5769-5774
Reproducible Dendronized PEG Hydrogels via SPAAC Cross-Linking
Authors: Hodgson, S. M., McNelles, S. A., Abdullahu, L., Marozas, I. A., Anseth, K. S., Adronov, A.
Journal: Biomacromolecules (2017): 4054-4059
Authors: Hodgson, S. M., McNelles, S. A., Abdullahu, L., Marozas, I. A., Anseth, K. S., Adronov, A.
Journal: Biomacromolecules (2017): 4054-4059
Fused Bicyclic Caspase-1 Inhibitors Assembled by Copper-Free Strain-Promoted Alkyne-Azide Cycloaddition (SPAAC)
Authors: Qian, L., Zhang, C. J., Wu, J., Yao, S. Q.
Journal: Chemistry (2017): 360-369
Authors: Qian, L., Zhang, C. J., Wu, J., Yao, S. Q.
Journal: Chemistry (2017): 360-369
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