Calculator
DAX-J2™ Red
DAX-J2™ Red is a new nitric oxide (NO) sensor recently developed by AAT Bioquest. It is a non-fluorescent cell permeable reagent that can measure free NO and nitric oxide synthase (NOS) activity in living cells under physiological conditions. Once inside the cell the blocking groups on the DAX-J2 reagent are released to generate the highly fluorescent product upon NO oxidation. DAX-J2 fluorescent product can be detected using the filter set of Texas Red® that is equipped with most of flow cytometers and fluorescence microscope. DAX-J2 Red has distinct advantages for NO detection than the popular DAF-2 NO probe: 1). It does not require esterase activity for NO detection. DAF-2 requires intracellular esterases to cleave its acetate groups for detecting NO activity. This esterase dependence often complicates the NO detection since esterase activities are affected by cell health and many other factors. 2). DAX-2 product exhibits pH-independent fluorescence while DAF-2 has its fluorescence highly affected by pH. 3). DAX-J2 Red fluorescent product is much more photostable than DAF-2 product. 4). It is more sensitive for detecting NO than DAF-2. 5). It can be used with GFP cell lines or with the applications that use FITC-labeled antibodies for multicolor cell analysis.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DAX-J2™ Red to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 164.266 µL | 821.328 µL | 1.643 mL | 8.213 mL | 16.427 mL |
| 5 mM | 32.853 µL | 164.266 µL | 328.531 µL | 1.643 mL | 3.285 mL |
| 10 mM | 16.427 µL | 82.133 µL | 164.266 µL | 821.328 µL | 1.643 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) |
| DAX-J2™ Orange | 552 | 575 |
Citations
View all 8 citations: Citation Explorer
Synthesis, structure, spectra, cytotoxicity and photo induced NO release of four isomeric nitrosylruthenium complexes
Authors: Shi, Jia and Liu, Yuhua and Jiao, Shuxiang and Wu, Tao and Wang, Ai and Wang, Wenming and Xie, Leilei and Liu, Yanhong and Wang, Hongfei
Journal: Nitric Oxide (2024)
Authors: Shi, Jia and Liu, Yuhua and Jiao, Shuxiang and Wu, Tao and Wang, Ai and Wang, Wenming and Xie, Leilei and Liu, Yanhong and Wang, Hongfei
Journal: Nitric Oxide (2024)
Low-dose daylight exposure induces nitric oxide release and maintains cell viability in vitro
Authors: Hazell, Gareth and Khazova, Marina
Journal: (2023)
Authors: Hazell, Gareth and Khazova, Marina
Journal: (2023)
Substance P-induced activation of presynaptic NK1 receptors suppresses EPSCs via nitric oxide synthesis in the rat insular cortex
Authors: 松村幸恵,
Journal: (2021)
Authors: 松村幸恵,
Journal: (2021)
Presynaptic NK1 Receptor Activation by Substance P Suppresses EPSCs via Nitric Oxide Synthesis in the Rat Insular Cortex
Authors: Matsumura, Sachie and Yamamoto, Kiyofumi and Nakaya, Yuka and O'Hashi, Kazunori and Kaneko, Keisuke and Takei, Hiroki and Tsuda, Hiromasa and Shirakawa, Tetsuo and Kobayashi, Masayuki
Journal: Neuroscience (2021): 151--164
Authors: Matsumura, Sachie and Yamamoto, Kiyofumi and Nakaya, Yuka and O'Hashi, Kazunori and Kaneko, Keisuke and Takei, Hiroki and Tsuda, Hiromasa and Shirakawa, Tetsuo and Kobayashi, Masayuki
Journal: Neuroscience (2021): 151--164
One-step preparation of the engineered titanium implant by rationally designed linear fusion peptides with spacer-dependent antimicrobial, anti-inflammatory and osteogenic activities
Authors: Xin, Haoqian and Chen, Junjian and Li, Tianjie and Hu, Guansong and Fang, Zhou and Zhou, Haiyan and Guo, Kunzhong and Wang, Lin and Wang, Yingjun
Journal: Chemical Engineering Journal (2021): 130380
Authors: Xin, Haoqian and Chen, Junjian and Li, Tianjie and Hu, Guansong and Fang, Zhou and Zhou, Haiyan and Guo, Kunzhong and Wang, Lin and Wang, Yingjun
Journal: Chemical Engineering Journal (2021): 130380
References
View all 139 references: Citation Explorer
Pitfalls and limitations in using 4,5-diaminofluorescein for evaluating the influence of polyphenols on nitric oxide release from endothelial cells
Authors: Uhlenhut K, Hogger P.
Journal: Free Radic Biol Med (2012): 2266
Authors: Uhlenhut K, Hogger P.
Journal: Free Radic Biol Med (2012): 2266
Effects of moderate electrical stimulation on reactive species production by primary rat skeletal muscle cells: cross talk between superoxide and nitric oxide production
Authors: Lambertucci RH, Silveira Ldos R, Hirabara SM, Curi R, Sweeney G, Pithon-Curi TC.
Journal: J Cell Physiol (2012): 2511
Authors: Lambertucci RH, Silveira Ldos R, Hirabara SM, Curi R, Sweeney G, Pithon-Curi TC.
Journal: J Cell Physiol (2012): 2511
Improved measurements of intracellular nitric oxide in intact microvessels using 4,5-diaminofluorescein diacetate
Authors: Zhou X, He P.
Journal: Am J Physiol Heart Circ Physiol (2011): H108
Authors: Zhou X, He P.
Journal: Am J Physiol Heart Circ Physiol (2011): H108
Aging negatively affects estrogens-mediated effects on nitric oxide bioavailability by shifting ERalpha/ERbeta balance in female mice
Authors: Novensa L, Novella S, Medina P, Segarra G, Castillo N, Heras M, Hermenegildo C, Dantas AP.
Journal: PLoS One (2011): e25335
Authors: Novensa L, Novella S, Medina P, Segarra G, Castillo N, Heras M, Hermenegildo C, Dantas AP.
Journal: PLoS One (2011): e25335
Temporal and spatial correlation of platelet-activating factor-induced increases in endothelial [Ca(2)(+)]i, nitric oxide, and gap formation in intact venules
Authors: Zhou X, He P.
Journal: Am J Physiol Heart Circ Physiol (2011): H1788
Authors: Zhou X, He P.
Journal: Am J Physiol Heart Circ Physiol (2011): H1788
Page updated on November 21, 2024
Safety data sheet