DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 350.25 |
Solvent | Water |
Spectral properties
Extinction coefficient (cm -1 M -1) | 27000 |
Excitation (nm) | 359 |
Emission (nm) | 457 |
Storage, safety and handling
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H340 |
Hazard symbol | T |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R68 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 41116134 |
Overview | SDSProtocol |
See also: Cell Cycle Assays, Nucleus, DAPI - Blue Fluorescent DNA Stain, Fluorescence Activated Cell Sorting (FACS), Immunohistochemistry (IHC)
CAS 28718-90-3 | Molecular weight 350.25 | Extinction coefficient (cm -1 M -1) 27000 | Excitation (nm) 359 | Emission (nm) 457 |
DAPI is a fluorescent stain that binds strongly to DNA. It is used extensively in fluorescence microscopy. Since DAPI passes through an intact cell membrane, it can be used to stain live cells besides fixed cells. For fluorescence microscopy, DAPI is excited with ultraviolet light. When bound to double-stranded DNA its absorption maximum is at 358 nm and its emission maximum is at 461 nm. One drawback of DAPI is that its emission is fairly broad. DAPI also binds to RNA although it is not as strongly fluorescent as it binds to DNA. Its emission shifts to around 500 nm when bound to RNA. DAPI's blue emission is convenient for multiplexing assays since there is very little fluorescence overlap between DAPI and green-fluorescent molecules like fluorescein and green fluorescent protein (GFP), or red-fluorescent stains like Texas Red. Besides labeling cell nuclei, DAPI is also used for the detection of mycoplasma or virus DNA in cell cultures.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 285.51 µL | 1.428 mL | 2.855 mL | 14.276 mL | 28.551 mL |
5 mM | 57.102 µL | 285.51 µL | 571.021 µL | 2.855 mL | 5.71 mL |
10 mM | 28.551 µL | 142.755 µL | 285.51 µL | 1.428 mL | 2.855 mL |
Molarity calculator
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Extinction coefficient (cm -1 M -1) | 27000 |
Excitation (nm) | 359 |
Emission (nm) | 457 |
Images
Citations
View all 85 citations: Citation Explorer
Deciphering the Role of Ferroptosis in Rheumatoid Arthritis: Synovial Transcriptome Analysis and Immune Infiltration Correlation
Authors: Wang, Hongli and Zhang, Miaomiao and Hu, Yiping and He, Juan and Zhong, Yuchao and Dai, Yong and Wang, Qingwen
Journal: Heliyon (2024)
Authors: Wang, Hongli and Zhang, Miaomiao and Hu, Yiping and He, Juan and Zhong, Yuchao and Dai, Yong and Wang, Qingwen
Journal: Heliyon (2024)
The mesenchymal stromal cell (MSC) secretome promotes tissue regeneration and increases macrophage infiltration in acute and MRSA-infected skin wounds in vivo
Authors: Rajesh, Aarthi and Ju, Esther Da Eun and Oxford, Kelly A and Harman, Rebecca M and Van de Walle, Gerlinde R
Journal: Cytotherapy (2024)
Authors: Rajesh, Aarthi and Ju, Esther Da Eun and Oxford, Kelly A and Harman, Rebecca M and Van de Walle, Gerlinde R
Journal: Cytotherapy (2024)
Adipose Tissue and Translation Machinery in Metabolic Regulation
Authors: De Siqueira, Mirian Krystel
Journal: (2024)
Authors: De Siqueira, Mirian Krystel
Journal: (2024)
Reciprocal conversion between annual and polycarpic perennial flowering behavior in the Brassicaceae
Authors: Zhai, Dong and Zhang, Lu-Yi and Li, Ling-Zi and Xu, Zhou-Geng and Liu, Xiao-Li and Shang, Guan-Dong and Zhao, Bo and Gao, Jian and Wang, Fu-Xiang and Wang, Jia-Wei
Journal: Cell (2024)
Authors: Zhai, Dong and Zhang, Lu-Yi and Li, Ling-Zi and Xu, Zhou-Geng and Liu, Xiao-Li and Shang, Guan-Dong and Zhao, Bo and Gao, Jian and Wang, Fu-Xiang and Wang, Jia-Wei
Journal: Cell (2024)
A novel HDAC8 inhibitor H7E exerts retinoprotective effects against glaucomatous injury via ameliorating aberrant M{\"u}ller glia activation and oxidative stress
Authors: Wu, Liang-Huan and Cheng, Yu-Wen and Lin, Fan-Li and Hsu, Kai-Cheng and Wang, Mong-Heng and Yen, Jing-Lun and Wang, Tsung-Jen and Lin, Tony Eight and Liu, Yi-Chien and Huang, Wei-Jan and others,
Journal: Biomedicine \& Pharmacotherapy (2024): 116538
Authors: Wu, Liang-Huan and Cheng, Yu-Wen and Lin, Fan-Li and Hsu, Kai-Cheng and Wang, Mong-Heng and Yen, Jing-Lun and Wang, Tsung-Jen and Lin, Tony Eight and Liu, Yi-Chien and Huang, Wei-Jan and others,
Journal: Biomedicine \& Pharmacotherapy (2024): 116538
A xenotransplantation mouse model to study physiology of the mammary gland from large mammals
Authors: Miller, James L and Reddy, Alexandra and Harman, Rebecca M and Van de Walle, Gerlinde R
Journal: Plos one (2024): e0298390
Authors: Miller, James L and Reddy, Alexandra and Harman, Rebecca M and Van de Walle, Gerlinde R
Journal: Plos one (2024): e0298390
Cell softness renders cytotoxic T lymphocytes and T leukemic cells resistant to perforin-mediated killing
Authors: Zhou, Yabo and Wang, Dianheng and Zhou, Li and Zhou, Nannan and Wang, Zhenfeng and Chen, Jie and Pang, Ruiyang and Fu, Haixia and Huang, Qiusha and Dong, Fang and others,
Journal: Nature Communications (2024): 1405
Authors: Zhou, Yabo and Wang, Dianheng and Zhou, Li and Zhou, Nannan and Wang, Zhenfeng and Chen, Jie and Pang, Ruiyang and Fu, Haixia and Huang, Qiusha and Dong, Fang and others,
Journal: Nature Communications (2024): 1405
Differential impact of cytoplasmic vs. nuclear RAD51 expression on breast cancer progression and patient prognosis
Authors: Wang, Yen-Yun and Cheng, Kuang-Hung and Hung, Amos C and Lo, Steven and Chen, Pang-Yu and Wu, Yi-Chia and Hou, Ming-Feng and Yuan, Shyng-Shiou F
Journal: International Journal of Oncology (2024): 1--16
Authors: Wang, Yen-Yun and Cheng, Kuang-Hung and Hung, Amos C and Lo, Steven and Chen, Pang-Yu and Wu, Yi-Chia and Hou, Ming-Feng and Yuan, Shyng-Shiou F
Journal: International Journal of Oncology (2024): 1--16
Co-Delivery of Bioengineered Exosomes and Oxygen for Treating Critical Limb Ischemia in Diabetic Mice
Authors: Zhong, Ting and Gao, Ning and Guan, Ya and Liu, Zhongting and Guan, Jianjun
Journal: ACS nano (2023)
Authors: Zhong, Ting and Gao, Ning and Guan, Ya and Liu, Zhongting and Guan, Jianjun
Journal: ACS nano (2023)
Alcaligene s Lipid A Functions as a Superior Mucosal Adjuvant to Monophosphoryl Lipid A via the Recruitment and Activation of CD11b+ Dendritic Cells in Nasal Tissue
Authors: Sun, Xiao and Hosomi, Koji and Shimoyama, Atsushi and Yoshii, Ken and Saika, Azusa and Yamaura, Haruki and Nagatake, Takahiro and Kiyono, Hiroshi and Fukase, Koichi and Kunisawa, Jun
Journal: International Immunology (2023): dxad045
Authors: Sun, Xiao and Hosomi, Koji and Shimoyama, Atsushi and Yoshii, Ken and Saika, Azusa and Yamaura, Haruki and Nagatake, Takahiro and Kiyono, Hiroshi and Fukase, Koichi and Kunisawa, Jun
Journal: International Immunology (2023): dxad045
References
View all 134 references: Citation Explorer
Reliable DNA ploidy determination in dehydrated tissues of vascular plants by DAPI flow cytometry--new prospects for plant research
Authors: Suda J, Travnicek P.
Journal: Cytometry A (2006): 273
Authors: Suda J, Travnicek P.
Journal: Cytometry A (2006): 273
Identification of all pachytene bivalents in the common shrew using DAPI-staining of synaptonemal complex spreads
Authors: Belonogova NM, Karamysheva TV, Biltueva LS, Perepelov EA, Minina JM, Polyakov AV, Zhdanova NS, Rubtsov NB, Searle JB, Borodin PM.
Journal: Chromosome Res (2006): 673
Authors: Belonogova NM, Karamysheva TV, Biltueva LS, Perepelov EA, Minina JM, Polyakov AV, Zhdanova NS, Rubtsov NB, Searle JB, Borodin PM.
Journal: Chromosome Res (2006): 673
Physical localization of ribosomal genes and chromosome DAPI banding by in situ hybridization in Medicago sativa L
Authors: Chen JM, Hong YH, Wang YP, Bowley S, Wan JM.
Journal: Yi Chuan (2006): 184
Authors: Chen JM, Hong YH, Wang YP, Bowley S, Wan JM.
Journal: Yi Chuan (2006): 184
DNA staining with the fluorochromes EtBr, DAPI and YOYO-1 in the comet assay with tobacco plants after treatment with ethyl methanesulphonate, hyperthermia and DNase-I
Authors: Gichner T, Mukherjee A, Veleminsky J.
Journal: Mutat Res (2006): 17
Authors: Gichner T, Mukherjee A, Veleminsky J.
Journal: Mutat Res (2006): 17
Investigation of chromosomes in varieties and translocation lines of pea Pisum sativum L. by FISH, Ag-NOR, and differential DAPI staining
Authors: Samatadze TE, Muravenko OM, Bol'sheva NL, Amosova AB, Gostimsckii SA, Zelenin AV.
Journal: Genetika (2005): 1665
Authors: Samatadze TE, Muravenko OM, Bol'sheva NL, Amosova AB, Gostimsckii SA, Zelenin AV.
Journal: Genetika (2005): 1665
DAPI fluorescence in nuclei isolated from tumors
Authors: Krishan A, D and ekar PD., undefined
Journal: J Histochem Cytochem (2005): 1033
Authors: Krishan A, D and ekar PD., undefined
Journal: J Histochem Cytochem (2005): 1033
Polarized fluorescence correlation spectroscopy of DNA-DAPI complexes
Authors: Barcellona ML, Gammon S, Hazlett T, Digman MA, Gratton E.
Journal: Microsc Res Tech (2004): 205
Authors: Barcellona ML, Gammon S, Hazlett T, Digman MA, Gratton E.
Journal: Microsc Res Tech (2004): 205
DNA quantification in nuclei of cultivated mushroom with DAPI staining
Authors: Pancheva EV, Volkova VN, Kamzolkina OV.
Journal: Tsitologiia (2004): 381
Authors: Pancheva EV, Volkova VN, Kamzolkina OV.
Journal: Tsitologiia (2004): 381
Quantification of sPLA2-induced early and late apoptosis changes in neuronal cell cultures using combined TUNEL and DAPI staining
Authors: Daniel B, DeCoster MA.
Journal: Brain Res Brain Res Protoc (2004): 144
Authors: Daniel B, DeCoster MA.
Journal: Brain Res Brain Res Protoc (2004): 144
Interaction of DAPI with individual strands of trinucleotide repeats. Effects of replication in vitro of the AAT x ATT triplet
Authors: Trotta E, Del Grosso N, Erba M, Melino S, Cicero D, Paci M.
Journal: Eur J Biochem (2003): 4755
Authors: Trotta E, Del Grosso N, Erba M, Melino S, Cicero D, Paci M.
Journal: Eur J Biochem (2003): 4755
Application notes
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