DAF-FM diacetate
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 496.42 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
See also: Reactive Oxygen Species (ROS)
CAS 254109-22-3 | Molecular weight 496.42 |
DAF-FM diacetate is a more cell-permeable version of DAF-FM, a sensitive fluorescent indicator for the detection and bioimaging of nitric oxide (NO). Upon entry into cells, DAF-FM diacetate is hydrolyzed to the less cell-permeable DAF-FM by cellular esterases, thus preventing loss of signal due to leakage of DAF-FM diacetate from cells. In the presence of oxygen, DAF-FM reacts with NO to yield the highly fluorescent difluoro-triazolofluorescein. The intracellular fluorescence (generated by DAF-FM diacetate) can be readily monitored using the standard FITC filter set. DAF-FM diacetate can be utilized in cells for a broad range of NO production, e.g., the small amounts of NO production (such as in endothelial cells) and large amounts of NO production (such as in macrophages). Compared with DAF-2 DA, DAF-FM diacetate is a much more sensitive and photo-stable fluorescent probe for detecting NO. In addition, DAF-FM is much less pH sensitive than DAF-2, essentially pH-independent in the physiological pH range.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DAF-FM diacetate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 201.442 µL | 1.007 mL | 2.014 mL | 10.072 mL | 20.144 mL |
5 mM | 40.288 µL | 201.442 µL | 402.885 µL | 2.014 mL | 4.029 mL |
10 mM | 20.144 µL | 100.721 µL | 201.442 µL | 1.007 mL | 2.014 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
References
View all 2 references: Citation Explorer
A reliable fluorimetric method to screen the nitric oxide synthase inhibitors in 96 well plate.
Authors: Agrawal, Saurabh and Kumari, Rita and Luthra, Pratibha Mehta
Journal: Analytical biochemistry (2019): 42-44
Authors: Agrawal, Saurabh and Kumari, Rita and Luthra, Pratibha Mehta
Journal: Analytical biochemistry (2019): 42-44
Modification of endoplasmic reticulum Ca2+ stores by select oxidants produces changes reminiscent of those in cells from patients with Alzheimer disease.
Authors: Huang, Hsueh-Meei and Chen, Huan-Lian and Xu, Hui and Gibson, Gary E
Journal: Free radical biology & medicine (2005): 979-89
Authors: Huang, Hsueh-Meei and Chen, Huan-Lian and Xu, Hui and Gibson, Gary E
Journal: Free radical biology & medicine (2005): 979-89
Application notes
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Abbreviation of Common Chemical Compounds Related to Peptides
Attenuation of lysyl oxidase and collagen gene expression in keratoconus patient corneal epithelium corresponds to disease severity
Copper Transport Protein Antioxidant-1 Promotes Inflammatory Neovascularization via Chaperone and Transcription Factor Function
Dopamine-Mediated Oxidation of Methionine 127 in α-Synuclein
Abbreviation of Common Chemical Compounds Related to Peptides
Attenuation of lysyl oxidase and collagen gene expression in keratoconus patient corneal epithelium corresponds to disease severity
Copper Transport Protein Antioxidant-1 Promotes Inflammatory Neovascularization via Chaperone and Transcription Factor Function
Dopamine-Mediated Oxidation of Methionine 127 in α-Synuclein