DAF-FM
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 412.35 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
See also: Reactive Oxygen Species (ROS)
CAS 254109-20-1 | Molecular weight 412.35 |
DAF-2 is the original compound and is widely used for detecting nitric oxide (NO) in cells. DAF-FM is an improved version of DAF. Compared with DAF-2, DAF-FM is a much more sensitive and photo-stable fluorescent probe for detecting NO. In addition, DAF-FM is much less pH sensitive than DAF-2, essentially pH-independent in the physiological pH range. DAF-FM is not quite as cell-permeable as the diacetate derivative, DAF-FM, but it does not need to be activated by cytosolic enzymes.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DAF-FM to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 242.512 µL | 1.213 mL | 2.425 mL | 12.126 mL | 24.251 mL |
5 mM | 48.502 µL | 242.512 µL | 485.025 µL | 2.425 mL | 4.85 mL |
10 mM | 24.251 µL | 121.256 µL | 242.512 µL | 1.213 mL | 2.425 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Citations
View all 1 citations: Citation Explorer
Mild hyperthermia enhanced synergistic uric acid degradation and multiple ROS elimination for an effective acute gout therapy
Authors: Zhao, Pei and Hu, Hua-Zhong and Chen, Xiao-Tong and Jiang, Qi-Yun and Yu, Xue-Zhao and Cen, Xiao-Lin and Lin, Shi-Qing and Mai, Sui-qing and Pang, Wei-lin and Chen, Jin-Xiang and others,
Journal: Journal of Nanobiotechnology (2024)
Authors: Zhao, Pei and Hu, Hua-Zhong and Chen, Xiao-Tong and Jiang, Qi-Yun and Yu, Xue-Zhao and Cen, Xiao-Lin and Lin, Shi-Qing and Mai, Sui-qing and Pang, Wei-lin and Chen, Jin-Xiang and others,
Journal: Journal of Nanobiotechnology (2024)
References
View all 50 references: Citation Explorer
Nitro-Oleic Acid in Seeds and Differently Developed Seedlings of Brassica napus L.
Authors: Vollár, Martin and Feigl, Gábor and Oláh, Dóra and Horváth, Attila and Molnár, Árpád and Kúsz, Norbert and Ördög, Attila and Csupor, Dezső and Kolbert, Zsuzsanna
Journal: Plants (Basel, Switzerland) (2020)
Authors: Vollár, Martin and Feigl, Gábor and Oláh, Dóra and Horváth, Attila and Molnár, Árpád and Kúsz, Norbert and Ördög, Attila and Csupor, Dezső and Kolbert, Zsuzsanna
Journal: Plants (Basel, Switzerland) (2020)
Omentin-1 protects against high glucose-induced endothelial dysfunction via the AMPK/PPARδ signaling pathway.
Authors: Liu, Fang and Fang, Shaohong and Liu, Xinxin and Li, Ji and Wang, Xuedong and Cui, Jinjin and Chen, Tao and Li, Zhaoying and Yang, Fan and Tian, Jiangtian and Li, Hulun and Yin, Li and Yu, Bo
Journal: Biochemical pharmacology (2020): 113830
Authors: Liu, Fang and Fang, Shaohong and Liu, Xinxin and Li, Ji and Wang, Xuedong and Cui, Jinjin and Chen, Tao and Li, Zhaoying and Yang, Fan and Tian, Jiangtian and Li, Hulun and Yin, Li and Yu, Bo
Journal: Biochemical pharmacology (2020): 113830
Association between expression of AMPK pathway and adiponectin, leptin, and vascular endothelial function in rats with coronary heart disease.
Authors: Li, J-M and Lu, W and Ye, J and Han, Y and Chen, H and Wang, L-S
Journal: European review for medical and pharmacological sciences (2020): 905-914
Authors: Li, J-M and Lu, W and Ye, J and Han, Y and Chen, H and Wang, L-S
Journal: European review for medical and pharmacological sciences (2020): 905-914
Methods for Measuring Nitrate Reductase, Nitrite Levels, and Nitric Oxide from Plant Tissues.
Authors: Wany, Aakanksha and Pathak, Pradeep Kumar and Gupta, Kapuganti Jagadis
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 15-26
Authors: Wany, Aakanksha and Pathak, Pradeep Kumar and Gupta, Kapuganti Jagadis
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 15-26
Nitric oxide restores peripheral blood mononuclear cell adhesion against hypoxia via NO-cGMP signalling.
Authors: Behera, Jyotirmaya and Nagarajan, Shunmugam and Saran, Uttara and Kumar, Ravi and Keshri, Gaurav K and Suryakumar, Geetha and Chatterjee, Suvro
Journal: Cell biochemistry and function (2020)
Authors: Behera, Jyotirmaya and Nagarajan, Shunmugam and Saran, Uttara and Kumar, Ravi and Keshri, Gaurav K and Suryakumar, Geetha and Chatterjee, Suvro
Journal: Cell biochemistry and function (2020)
Histamine induces intracellular Ca2+ oscillations and nitric oxide release in endothelial cells from brain microvascular circulation.
Authors: Berra-Romani, Roberto and Faris, Pawan and Pellavio, Giorgia and Orgiu, Matteo and Negri, Sharon and Forcaia, Greta and Var-Gaz-Guadarrama, Verónica and Garcia-Carrasco, Mario and Botta, Laura and Sancini, Giulio and Laforenza, Umberto and Moccia, Francesco
Journal: Journal of cellular physiology (2020): 1515-1530
Authors: Berra-Romani, Roberto and Faris, Pawan and Pellavio, Giorgia and Orgiu, Matteo and Negri, Sharon and Forcaia, Greta and Var-Gaz-Guadarrama, Verónica and Garcia-Carrasco, Mario and Botta, Laura and Sancini, Giulio and Laforenza, Umberto and Moccia, Francesco
Journal: Journal of cellular physiology (2020): 1515-1530
L-sepiapterin restores SLE serum-induced markers of endothelial function in endothelial cells.
Authors: Jones Buie, Joy N and Pleasant Jenkins, Dorea and Muise-Helmericks, Robin and Oates, Jim C
Journal: Lupus science & medicine (2019): e000294
Authors: Jones Buie, Joy N and Pleasant Jenkins, Dorea and Muise-Helmericks, Robin and Oates, Jim C
Journal: Lupus science & medicine (2019): e000294
Design, synthesis and biological study of hybrid drug candidates of nitric oxide releasing cucurbitacin-inspired estrone analogs for treatment of hepatocellular carcinoma.
Authors: Abou-Salim, Mahrous A and Shaaban, Mohamed A and Abd El Hameid, Mohammed K and Elshaier, Yaseen A M M and Halaweish, Fathi
Journal: Bioorganic chemistry (2019): 515-533
Authors: Abou-Salim, Mahrous A and Shaaban, Mohamed A and Abd El Hameid, Mohammed K and Elshaier, Yaseen A M M and Halaweish, Fathi
Journal: Bioorganic chemistry (2019): 515-533
Oxysterol modulates neurotransmission via liver-X receptor/NO synthase-dependent pathway at the mouse neuromuscular junctions.
Authors: Mukhutdinova, Kamilla A and Kasimov, Marat R and Zakyrjanova, Guzel F and Gumerova, Milausha R and Petrov, Alexey M
Journal: Neuropharmacology (2019): 70-79
Authors: Mukhutdinova, Kamilla A and Kasimov, Marat R and Zakyrjanova, Guzel F and Gumerova, Milausha R and Petrov, Alexey M
Journal: Neuropharmacology (2019): 70-79
Androgens drive microvascular endothelial dysfunction in women with polycystic ovary syndrome: role of the endothelin B receptor.
Authors: Usselman, Charlotte W and Yarovinsky, Timur O and Steele, Frances E and Leone, Cheryl A and Taylor, Hugh S and Bender, Jeffrey R and Stachenfeld, Nina S
Journal: The Journal of physiology (2019): 2853-2865
Authors: Usselman, Charlotte W and Yarovinsky, Timur O and Steele, Frances E and Leone, Cheryl A and Taylor, Hugh S and Bender, Jeffrey R and Stachenfeld, Nina S
Journal: The Journal of physiology (2019): 2853-2865
Application notes
A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells