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DAF-2 DA
DAF-2 diacetate is a more cell-permeable version of DAF-2, a sensitive fluorescent indicator for the detection and bioimaging of nitric oxide (NO). Upon entry into cells, DAF-2 diacetate is hydrolyzed to the less cell-permeable DAF-2 by cellular esterases, thus preventing loss of signal due to leakage of DAF-2 diacetate from cells. In the presence of oxygen, DAF-2 reacts with NO to yield the highly fluorescent triazolofluorescein. The intracellular fluorescence (generated by DAF-2 diacetate) can be readily monitored using the standard FITC filter set. DAF-2 diacetate can be utilized in cells which produce small amounts of NO, such as endothelial cells, as well as in cells which generate large amounts of NO, such as macrophages.
Citations
View all 1 citations: Citation Explorer
Expanded characterization of in vitro polarized M0, M1, and M2 human monocyte-derived macrophages: Bioenergetic and secreted mediator profiles
Authors: Hickman, Elise and Smyth, Timothy and Cobos-Uribe, Catalina and Immormino, Robert and Rebuli, Meghan E and Moran, Timothy and Alexis, Neil E and Jaspers, Ilona
Journal: Plos one (2023): e0279037
Authors: Hickman, Elise and Smyth, Timothy and Cobos-Uribe, Catalina and Immormino, Robert and Rebuli, Meghan E and Moran, Timothy and Alexis, Neil E and Jaspers, Ilona
Journal: Plos one (2023): e0279037
References
View all 50 references: Citation Explorer
Involvement of nitrate reductase-dependent nitric oxide production in magnetopriming-induced salt tolerance in soybean.
Authors: Kataria, Sunita and Jain, Meeta and Tripathi, Durgesh K and Singh, Vijay P
Journal: Physiologia plantarum (2020): 422-436
Authors: Kataria, Sunita and Jain, Meeta and Tripathi, Durgesh K and Singh, Vijay P
Journal: Physiologia plantarum (2020): 422-436
Does Melatonin Exert Its Effect on Ram Sperm Capacitation Through Nitric Oxide Synthase Regulation?
Authors: Miguel-Jiménez, Sara and Carvajal-Serna, Melissa and Calvo, Silvia and Casao, Adriana and Cebrián-Pérez, José Álvaro and Muiño-Blanco, Teresa and Pérez-Pe, Rosaura
Journal: International journal of molecular sciences (2020)
Authors: Miguel-Jiménez, Sara and Carvajal-Serna, Melissa and Calvo, Silvia and Casao, Adriana and Cebrián-Pérez, José Álvaro and Muiño-Blanco, Teresa and Pérez-Pe, Rosaura
Journal: International journal of molecular sciences (2020)
Curcumin prevents strokes in stroke-prone spontaneously hypertensive rats by improving vascular endothelial function.
Authors: Lan, Cong and Chen, Xinjian and Zhang, Yuxun and Wang, Wei and Wang, Wei Eric and Liu, Yukai and Cai, Yue and Ren, Hongmei and Zheng, Shuo and Zhou, Lin and Zeng, Chunyu
Journal: BMC cardiovascular disorders (2018): 43
Authors: Lan, Cong and Chen, Xinjian and Zhang, Yuxun and Wang, Wei and Wang, Wei Eric and Liu, Yukai and Cai, Yue and Ren, Hongmei and Zheng, Shuo and Zhou, Lin and Zeng, Chunyu
Journal: BMC cardiovascular disorders (2018): 43
Nitric oxide in frozen-thawed equine sperm: Effects on motility, membrane integrity and sperm capacitation.
Authors: de Andrade, André F C and Arruda, Rubens P and Torres, Mariana A and Pieri, Naira C G and Leite, Ticiano G and Celeghini, Eneiva Carla C and Oliveira, Leticia Z and Gardés, Thayna P and Bussiere, Maria Clara C and Silva, Daniela F
Journal: Animal reproduction science (2018): 176-184
Authors: de Andrade, André F C and Arruda, Rubens P and Torres, Mariana A and Pieri, Naira C G and Leite, Ticiano G and Celeghini, Eneiva Carla C and Oliveira, Leticia Z and Gardés, Thayna P and Bussiere, Maria Clara C and Silva, Daniela F
Journal: Animal reproduction science (2018): 176-184
Inhibition of endothelial nitric oxide synthase decreases breast cancer cell MDA-MB-231 adhesion to intact microvessels under physiological flows.
Authors: Zhang, Lin and Zeng, Min and Fu, Bingmei M
Journal: American journal of physiology. Heart and circulatory physiology (2016): H1735-47
Authors: Zhang, Lin and Zeng, Min and Fu, Bingmei M
Journal: American journal of physiology. Heart and circulatory physiology (2016): H1735-47
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