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AAT Bioquest

CytoFix™ Red Lysosomal Stain

Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste and cellular debris through a process known as autophagy. AAT Bioquest offers CytoFix™ Red lysosomal stain for selectively staining lysosomes. CytoFix™ Red lysosomal stain is well retained in lysosomes even after fixation. The dye permeates intact live cells and gets trapped in lysosomes. The fluorescence in lysosomes generated by this dye is well retained at least for 1 week, making it an excellent lysosomal tracking dye. The key features of this stain are its high staining efficiency, long retention after fixation with minimal hands on time. CytoFix™ Red lysosomal stain can be used with GFP expressed cells or with other organelles stains for multicolor analysis. It can be used for both suspension and adherent cells and readily adapted for a wide variety of fluorescence platforms.

Spectrum

Product family

NameExcitation (nm)Emission (nm)
CytoFix™ Red Mitochondrial Stain564651
Page updated on December 17, 2024

Ordering information

Price
Unit size
Catalog Number23210
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Request quotation

Physical properties

Molecular weight

863.75

Solvent

DMSO

Spectral properties

Excitation (nm)

565

Emission (nm)

585

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

N/A

Platform

Fluorescence microscope

ExcitationCy3, TRITC filter set
EmissionCy3, TRITC filter set
Recommended plateBlack wall, clear bottom
Instrument specification(s)Cy3, TRITC filter set
The fluorescence images of HeLa cells stained with CytoFix&trade; LysoRed in a 96-well black-wall clear-bottom plate. Image was acquired before (Left) and after (Right) fixation with 4% formaldehyde solution for 20 minutes at RT. The cells were imaged using fluorescence microscope with a Cy3/TRITC filter.<br>&nbsp;
The fluorescence images of HeLa cells stained with CytoFix&trade; LysoRed in a 96-well black-wall clear-bottom plate. Image was acquired before (Left) and after (Right) fixation with 4% formaldehyde solution for 20 minutes at RT. The cells were imaged using fluorescence microscope with a Cy3/TRITC filter.<br>&nbsp;
The fluorescence images of HeLa cells stained with CytoFix&trade; LysoRed in a 96-well black-wall clear-bottom plate. Image was acquired before (Left) and after (Right) fixation with 4% formaldehyde solution for 20 minutes at RT. The cells were imaged using fluorescence microscope with a Cy3/TRITC filter.<br>&nbsp;