CytoCalcein™ Violet 660, sodium salt Excited at 405 nm

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Fax	1-800-609-2943
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Physical properties

Molecular weight	662.63
Solvent	DMSO

Spectral properties

Excitation (nm)	402
Emission (nm)	660

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Overview SDS Protocol

Molecular weight

662.63

Excitation (nm)

402

Emission (nm)

660

CytoCalcein™ Violet 660 is the esterase-cleaved product of CytoCalcein™ Violet 660 AM in live cells. CytoCalcein™ Violet 660 is not cell-permeable. It is the reference product for the CytoCalcein™ Violet 660 AM that is designed for labeling live cells in the same way as Calcein, AM does. CytoCalcein™ Violet 660 is well excited with the Violet laser at 405 nm, which is now equipped in most flow cytometers. It is also well-excited by the excitation sources of fluorescence microscopes. This exceptional spectral separation from the typical existing FACS fluorophores provides additional options for multiplexing experiments with a flow cytometer or a fluorescence microscope. CytoCalcein™ Violet 660 has been developed for multiplexing flow cytometric and fluorescence imaging applications. It provides a new unique color for flow cytometric analysis of live cells.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CytoCalcein™ Violet 660, sodium salt *Excited at 405 nm* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	150.914 µL	754.569 µL	1.509 mL	7.546 mL	15.091 mL
5 mM	30.183 µL	150.914 µL	301.828 µL	1.509 mL	3.018 mL
10 mM	15.091 µL	75.457 µL	150.914 µL	754.569 µL	1.509 mL

Molarity calculator

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Spectrum

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Spectral properties

Excitation (nm)	402
Emission (nm)	660

References

View all 50 references: Citation Explorer

A Ratiometric Probe Based on Carbon Dots and Calcein & Eu3+ for the Fluorescent Detection of Sodium Tripolyphosphate.
Authors: Zheng, Yabin and Li, Chen and Li, Quan and Zhang, Taoyi and Chen, Jing and Ji, Wenxi and Wei, Yun
Journal: Journal of fluorescence (2023): 965-972

Dual-emission fluorescence detection of histidine using carbon dots and calcein/Ni2+ complexes.
Authors: Liu, Jinshui and Song, Bo and Fu, Ting
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2023): 121951

Construction of N-CDs and Calcein-Based Ratiometric Fluorescent Sensor for Rapid Detection of Arginine and Acetaminophen.
Authors: Qi, Haiyan and Li, Qiuying and Jing, Jing and Jing, Tao and Liu, Chuntong and Qiu, Lixin and Sami, Rokayya and Helal, Mahmoud and Ismail, Khadiga Ahmed and Aljahani, Amani H
Journal: Nanomaterials (Basel, Switzerland) (2022)

Tracking the Endosomal Escape: A Closer Look at Calcein and Related Reporters.
Authors: Hausig-Punke, Franziska and Richter, Friederike and Hoernke, Maria and Brendel, Johannes C and Traeger, Anja
Journal: Macromolecular bioscience (2022): e2200167

Sensitive ratiometric fluorescence probe based on chitosan carbon dots and calcein for Alkaline phosphatase detection and bioimaging in cancer cells.
Authors: Mu, Xiaowei and Jiang, Xue and Zhang, Yu and Liu, Xin and Zhang, Siqi and Wang, Wei and Huang, Yibing and Ma, Pinyi and Song, Daqian
Journal: Analytica chimica acta (2021): 339163

Calcein Binding to Assess Mineralization in Hydrogel Microspheres.
Authors: White, Kristopher and Chalaby, Rabab and Lowe, Gina and Berlin, Jacob and Glackin, Carlotta and Olabisi, Ronke
Journal: Polymers (2021)

Facile Ultrasound-Triggered Release of Calcein and Doxorubicin from Iron-Based Metal-Organic Frameworks.
Authors: Ibrahim, Mihad and Sabouni, Rana and Husseini, Ghaleb A and Karami, Abdollah and Bai, Renu Geetha and Mukhopadhyay, Debasmita
Journal: Journal of biomedical nanotechnology (2020): 1359-1369

Calcein release assay as a method for monitoring serum complement activity during monoclonal antibody therapy in patients with B-cell malignancies.
Authors: Stasiłojć, Grzegorz and Felberg, Anna and Urban, Aleksandra and Kowalska, Daria and Ma, Shuo and Blom, Anna M and Lundin, Jeanette and Österborg, Anders and Okrój, Marcin
Journal: Journal of immunological methods (2020): 112675

The Relationship Between Water Transmission Rate and Defects on the Film Based on the Defect Analysis Using Fluorescent Calcein Probe.
Authors: Kim, Sun Min and Kim, Hyun Chul and Kim, Chang-Yeoul and Yoon, Daeho and Koo, Eunhae
Journal: Journal of nanoscience and nanotechnology (2020): 5469-5472

Calcein Release Assay to Measure Membrane Permeabilization by Recombinant Alpha-Synuclein.
Authors: Dutta, Sayan and Watson, Ben G and Mattoo, Seema and Rochet, Jean-Christophe
Journal: Bio-protocol (2020)