CypHer7E NHS Ester

CypHer7E NHS ester is an excellent building block for developing site-specific pH probes. It is analogous to CypHer5E NHS ester with much longer excitation and emission. It is one of the very few acidotropic pH probes that exhibit NIR fluorescence. It can be readily conjugated to amino-containing biomolecules (such as antibodies, peptides and amino-modified oligos). CypHer7E is a NIR pH-sensitive cyanine dye derivative that can be used to study most classes of cell surface receptors which internalize into the endocytic pathway when activated by a ligand. CypHer7E NHS ester is a flexible, generic fluorophore that can be coupled to a receptor or epitope tag antibody and monitors neutral to acidic intracellular events.

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (260 nm)	Correction Factor (280 nm)
XFD488 NHS Ester Same Structure to Alexa Fluor™ 488 NHS Ester	499	520	71000	0.30	0.11
XFD350 NHS Ester Same Structure to Alexa Fluor™ 350 NHS Ester	343	441	19000	0.25	0.19
XFD532 NHS Ester Same Structure to Alexa Fluor™ 532 NHS Ester	534	553	81000	0.24	0.09
XFD594 NHS Ester Same Structure to Alexa Fluor™ 594 NHS Ester	590	618	90000	0.43	0.56
QXY21 NHS ester [equivalent to QSY-21 NHS ester]	-	-	89000¹	-	0.32
XFD555 NHS Ester Same Structure to Alexa Fluor™ 555 NHS Ester	553	568	150000	0.08	0.08
XFD647 NHS Ester Same Structure to Alexa Fluor™ 647 NHS Ester	650	671	239000	0.00	0.03
XFD680 NHS Ester Same Structure to Alexa Fluor™ 680 NHS Ester	681	704	184000	0.00	0.05
XFD700 NHS Ester Same Structure to Alexa Fluor™ 700 NHS Ester	696	719	192000	0.00	0.07
XFD750 NHS Ester Same Structure to Alexa Fluor™ 750 NHS Ester	752	776	240000	0.00	0.04
Cy5DIGE NHS ester	651	670	250000¹	0.02	0.03
Cy2DIGE NHS ester	492	508	150000	0.08	0.15
Cy3DIGE NHS ester	555	569	150000¹	0.07	0.073
XFD546 NHS Ester Same Structure to Alexa Fluor™ 546 NHS Ester	561	572	112000	0.21	0.12
XFD568 NHS Ester Same Structure to Alexa Fluor™ 568 NHS Ester	579	603	91300	0.45	0.46
XFD514 NHS Ester Same Structure to Alexa Fluor™ 514 NHS Ester	518	543	80000	0.31	0.18
QXY7 NHS ester [equivalent to QSY-7 NHS ester]	-	-	90000¹	-	0.22
Cy3B NHS ester	560	571	120000¹	0.048	0.069
XFD405 NHS Ester [equivalent to Alexa Fluor™ 405 NHS Ester]	401	421	35,000	0.23	0.70
CypHer5E NHS Ester	643	660	-	-	-
XFD660 NHS Ester [equivalent to Alexa Fluor® 660 NHS Ester]	663	691	132000	0.00	0.10
XFD430 NHS ester	432	540	15,000	-	0.28
XFD610 NHS Ester	611	629	144,000	0.43	0.44
XFD633 NHS Ester	632	650	159,000	-	0.55
XFD660 NHS Ester	663	691	132000	0.00	0.10
Show More (16)

References

View all 15 references: Citation Explorer

Altered exocytosis of inhibitory synaptic vesicles at single presynaptic terminals of cultured striatal neurons in a knock-in mouse model of Huntington's disease.
Authors: Xu, Chen and Chen, Sidong and Chen, Xingxiang and Ho, Ka Hei and Park, Chungwon and Yoo, Hanna and Lee, Suk-Ho and Park, Hyokeun
Journal: Frontiers in molecular neuroscience (2023): 1175522

Measuring Phagosomal pH by Fluorescence Microscopy.
Authors: Gonzales, Gerone A and Canton, Johnathan
Journal: Methods in molecular biology (Clifton, N.J.) (2023): 153-169

A near-infrared fluorescent pH sensing film for wound milieu pH monitoring.
Authors: Li, Shuxin and Vu, Hong and Senkowsky, Jon and Hu, Wenjing and Tang, Liping
Journal: Experimental dermatology (2020): 107-111

RNAi in Spodoptera frugiperda Sf9 Cells via Nanomaterial Mediated Delivery of dsRNA: A Comparison of Poly-l-arginine Polyplexes and Poly-l-arginine-Functionalized Au Nanoparticles.
Authors: Laisney, Jérôme and Gurusamy, Dhandapani and Baddar, Zeinah Elhaj and Palli, Subba Reddy and Unrine, Jason M
Journal: ACS applied materials & interfaces (2020): 25645-25657

Transport of orally delivered dsRNA in southern green stink bug, Nezara viridula.
Authors: Gurusamy, Dhandapani and Howell, Jeffrey L and Chereddy, Shankar C R R and Koo, Jinmo and Palli, Subba Reddy
Journal: Archives of insect biochemistry and physiology (2020): e21692

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