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CypHer5E Maleimide
CypHer5E maleimide is an excellent building block for developing site-specific pH probes. It can be readily conjugated to thiol-containing biomolecules (such as antibodies, peptides and thiol-modified oligos). CypHer5E is a red laser-excitable, pH-sensitive cyanine dye derivative that can be used to study most classes of cell surface receptors which internalize into the endocytic pathway when activated by a ligand. CypHer5E maleimide is a flexible, generic fluorophore that can be coupled to a receptor or epitope tag antibody and monitors neutral to acidic intracellular events. CypHer5E has an excitation peak that perfectly matches the red laser at 633 nm of He-Ne laser and 647 nm of red laser and an emission peak at ~670 nm.
Spectrum
References
View all 15 references: Citation Explorer
Altered exocytosis of inhibitory synaptic vesicles at single presynaptic terminals of cultured striatal neurons in a knock-in mouse model of Huntington's disease.
Authors: Xu, Chen and Chen, Sidong and Chen, Xingxiang and Ho, Ka Hei and Park, Chungwon and Yoo, Hanna and Lee, Suk-Ho and Park, Hyokeun
Journal: Frontiers in molecular neuroscience (2023): 1175522
Authors: Xu, Chen and Chen, Sidong and Chen, Xingxiang and Ho, Ka Hei and Park, Chungwon and Yoo, Hanna and Lee, Suk-Ho and Park, Hyokeun
Journal: Frontiers in molecular neuroscience (2023): 1175522
Measuring Phagosomal pH by Fluorescence Microscopy.
Authors: Gonzales, Gerone A and Canton, Johnathan
Journal: Methods in molecular biology (Clifton, N.J.) (2023): 153-169
Authors: Gonzales, Gerone A and Canton, Johnathan
Journal: Methods in molecular biology (Clifton, N.J.) (2023): 153-169
A near-infrared fluorescent pH sensing film for wound milieu pH monitoring.
Authors: Li, Shuxin and Vu, Hong and Senkowsky, Jon and Hu, Wenjing and Tang, Liping
Journal: Experimental dermatology (2020): 107-111
Authors: Li, Shuxin and Vu, Hong and Senkowsky, Jon and Hu, Wenjing and Tang, Liping
Journal: Experimental dermatology (2020): 107-111
RNAi in Spodoptera frugiperda Sf9 Cells via Nanomaterial Mediated Delivery of dsRNA: A Comparison of Poly-l-arginine Polyplexes and Poly-l-arginine-Functionalized Au Nanoparticles.
Authors: Laisney, Jérôme and Gurusamy, Dhandapani and Baddar, Zeinah Elhaj and Palli, Subba Reddy and Unrine, Jason M
Journal: ACS applied materials & interfaces (2020): 25645-25657
Authors: Laisney, Jérôme and Gurusamy, Dhandapani and Baddar, Zeinah Elhaj and Palli, Subba Reddy and Unrine, Jason M
Journal: ACS applied materials & interfaces (2020): 25645-25657
Transport of orally delivered dsRNA in southern green stink bug, Nezara viridula.
Authors: Gurusamy, Dhandapani and Howell, Jeffrey L and Chereddy, Shankar C R R and Koo, Jinmo and Palli, Subba Reddy
Journal: Archives of insect biochemistry and physiology (2020): e21692
Authors: Gurusamy, Dhandapani and Howell, Jeffrey L and Chereddy, Shankar C R R and Koo, Jinmo and Palli, Subba Reddy
Journal: Archives of insect biochemistry and physiology (2020): e21692
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