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Cyluc1 [(4S)-2-(6,7-Dihydro-5H-thiazolo[4,5-f]indol-2-yl)-4,5-dihydro-thiazole-4-carboxylic acid]
Cyluc1 is a synthetic cyclic alkylaminoluciferin that exhibits properties that are superior to those of D-luciferin for in vivo imaging due to its red-shifted luminescence. It binds to luciferase with higher affinity and and emits about 5.7-fold more light than aminoluciferin and 3.2-fold more light than D-luciferin. Its superior light output is maintained over a wide range (1-100 µM). It has been demonstrated that Cyluc1 readily crosses the blood-brain barrier in mice and provides much improved noninvasive bioluminescence imaging (BLI) of the brain at lower dose when compared to D-luciferin. It yields about 10-fold higher bioluminescent signal than D-luciferin when Luc2 luciferase expressing 4T1 breast cancer cells are implanted into the mammary fat pads of BABL/c mice. A rapid (4-5 min), long-lasting, and steady signal peak is observed following an i.v injection CycLuc1 in mice.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Cyluc1 [(4S)-2-(6,7-Dihydro-5H-thiazolo[4,5-f]indol-2-yl)-4,5-dihydro-thiazole-4-carboxylic acid] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 327.472 µL | 1.637 mL | 3.275 mL | 16.374 mL | 32.747 mL |
| 5 mM | 65.494 µL | 327.472 µL | 654.943 µL | 3.275 mL | 6.549 mL |
| 10 mM | 32.747 µL | 163.736 µL | 327.472 µL | 1.637 mL | 3.275 mL |
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References
View all 2 references: Citation Explorer
A synthetic luciferin improves in vivo bioluminescence imaging of gene expression in cardiovascular brain regions
Authors: Simonyan, H.; Hurr, C.; Young, C. N.
Journal: Physiol Genomics (2016): 762-770
Authors: Simonyan, H.; Hurr, C.; Young, C. N.
Journal: Physiol Genomics (2016): 762-770
Robust light emission from cyclic alkylaminoluciferin substrates for firefly luciferase
Authors: Reddy, G. R.; Thompson, W. C.; Miller, S. C.
Journal: J Am Chem Soc (2010): 13586-7
Authors: Reddy, G. R.; Thompson, W. C.; Miller, S. C.
Journal: J Am Chem Soc (2010): 13586-7
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