Cyanine 7.5 monosuccinimidyl ester [equivalent to Cy7.5® NHS ester]
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1343.73 |
Solvent | DMSO |
Spectral properties
Extinction coefficient (cm -1 M -1) | 250000 |
Excitation (nm) | 785 |
Emission (nm) | 801 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
Molecular weight 1343.73 | Extinction coefficient (cm -1 M -1) 250000 | Excitation (nm) 785 | Emission (nm) 801 |
Cyanine dyes are widely utilized for labeling biological molecules, particularly peptides, proteins, and oligos, in fluorescence imaging and other fluorescence-based biochemical analyses. Among these, Cyanine 7.5 (Cy7.5) NHS ester is notable for its near-infrared (NIR) properties, exhibiting long-wave emission characteristics. The NHS ester (or succinimidyl ester) is favored for its efficient reaction with the primary amines of proteins (Lys), peptides, amine-modified oligonucleotides, and other amine-containing molecules. Cy7.5 NHS Ester allows for high molar ratios of labeling without significant self-quenching, leading to brighter NIR conjugates and improved detection sensitivity. These conjugates demonstrate pH insensitivity over a wide range of molar concentrations, ensuring consistent signal generation in imaging and flow cytometry applications. Moreover, the near-infrared emission of Cy7.5 enables deep tissue penetration, making it ideal for in vivo imaging and tracking distribution in organisms. AAT Bioquest offers cyanine dye NHS esters in triethylammonium salt form, which exhibit superior solubility in dimethyl sulfoxide (DMSO) and dimethylformamide (DMF) compared to potassium salt formulations offered by other vendors. The reactivity of the cyanine dye triethylammonium salts is equivalent to that of cyanine dye potassium salts, producing conjugates with identical properties.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Cyanine 7.5 monosuccinimidyl ester [equivalent to Cy7.5® NHS ester] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 74.42 µL | 372.099 µL | 744.197 µL | 3.721 mL | 7.442 mL |
5 mM | 14.884 µL | 74.42 µL | 148.839 µL | 744.197 µL | 1.488 mL |
10 mM | 7.442 µL | 37.21 µL | 74.42 µL | 372.099 µL | 744.197 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
Open in Advanced Spectrum Viewer
![spectrum](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fspectra%2Fcy7_5_cyanine_7_5_.png&w=2048&q=50)
![spectrum](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fspectra%2Fcy7_5_cyanine_7_5_.png&w=2048&q=50)
Spectral properties
Extinction coefficient (cm -1 M -1) | 250000 |
Excitation (nm) | 785 |
Emission (nm) | 801 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) | Correction Factor (482 nm) | Correction Factor (565 nm) | Correction Factor (650 nm) |
Cyanine 3.5 monosuccinimidyl ester [equivalent to Cy3.5® NHS ester] | 579 | 591 | 150000 | 0.15 | 0.08 | 0.178 | - | - | - |
Cyanine 5.5 monosuccinimidyl ester [equivalent to Cy5.5® NHS ester] | 683 | 703 | 250000 | 0.27 | 0.05 | 0.101 | 0.0017 | 0.047 | 0.454 |
Images
References
View all 29 references: Citation Explorer
[Antitumor effects of redox-responsive nanoparticles containing platinum(Ⅳ)in ovarian cancer].
Authors: Hou, H Y and Tang, D S and Zhang, Y N and Wang, K Y and Ao, M and Luo, H X and Li, B
Journal: Zhonghua zhong liu za zhi [Chinese journal of oncology] (2024): 76-85
Authors: Hou, H Y and Tang, D S and Zhang, Y N and Wang, K Y and Ao, M and Luo, H X and Li, B
Journal: Zhonghua zhong liu za zhi [Chinese journal of oncology] (2024): 76-85
Polysaccharide dextran-based conjugate for selective co-delivery of two synergistic drugs docetaxel and docosahexaenoic acid to tumor cells.
Authors: Dong, Peng and Lv, Hongshuai and Jia, Weiping and Liu, Jiaojiao and Wang, Si and Li, Xiaohai and Hu, Jinghua and Zhao, Ling and Shi, Yikang
Journal: Drug delivery (2023): 40-50
Authors: Dong, Peng and Lv, Hongshuai and Jia, Weiping and Liu, Jiaojiao and Wang, Si and Li, Xiaohai and Hu, Jinghua and Zhao, Ling and Shi, Yikang
Journal: Drug delivery (2023): 40-50
Theoretical Study on Spectrum and Luminescence Mechanism of Cy5.5 and Cy7.5 Dye Based on Density Functional Theory (DFT).
Authors: Liu, Zhao and Meng, Xv and Zhang, Zhengze and Liu, Runzhang and Wang, Shutao and Lei, Jun-Qiang
Journal: Journal of fluorescence (2023)
Authors: Liu, Zhao and Meng, Xv and Zhang, Zhengze and Liu, Runzhang and Wang, Shutao and Lei, Jun-Qiang
Journal: Journal of fluorescence (2023)
Endovascular administration of magnetized nanocarriers targeting brain delivery after stroke.
Authors: Grayston, Alba and Zhang, Yajie and Garcia-Gabilondo, Miguel and Arrúe, Mercedes and Martin, Abraham and Kopcansky, Peter and Timko, Milan and Kovac, Jozef and Strbak, Oliver and Castellote, Laura and Belloli, Sara and Moresco, Rosa M and Picchio, Maria and Roig, Anna and Rosell, Anna
Journal: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabol (2022): 237-252
Authors: Grayston, Alba and Zhang, Yajie and Garcia-Gabilondo, Miguel and Arrúe, Mercedes and Martin, Abraham and Kopcansky, Peter and Timko, Milan and Kovac, Jozef and Strbak, Oliver and Castellote, Laura and Belloli, Sara and Moresco, Rosa M and Picchio, Maria and Roig, Anna and Rosell, Anna
Journal: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabol (2022): 237-252
PSMA-targeted NIR probes for image-guided detection of prostate cancer.
Authors: Cilibrizzi, Agostino and Wang, Julie Tzu-Wen and Memdouh, Siham and Iacovone, Antonella and McElroy, Kate and Jaffar, Noor and Young, Jennifer Denise and Hider, Robert C and Blower, Philip and Al-Jamal, Khuloud and Abbate, Vincenzo
Journal: Colloids and surfaces. B, Biointerfaces (2022): 112734
Authors: Cilibrizzi, Agostino and Wang, Julie Tzu-Wen and Memdouh, Siham and Iacovone, Antonella and McElroy, Kate and Jaffar, Noor and Young, Jennifer Denise and Hider, Robert C and Blower, Philip and Al-Jamal, Khuloud and Abbate, Vincenzo
Journal: Colloids and surfaces. B, Biointerfaces (2022): 112734
Tracking the gastrointestinal digestive and metabolic behaviour of Dendrobium officinale polysaccharides by fluorescent labelling.
Authors: Wu, Zhijing and Zhang, Yu and Nie, Gang and Liu, Junxi and Mei, Hao and He, Zihao and Dou, Pengfei and Wang, Kaiping
Journal: Food & function (2022): 7274-7286
Authors: Wu, Zhijing and Zhang, Yu and Nie, Gang and Liu, Junxi and Mei, Hao and He, Zihao and Dou, Pengfei and Wang, Kaiping
Journal: Food & function (2022): 7274-7286
Comparison between Fluorescence Imaging and Elemental Analysis to Determine Biodistribution of Inorganic Nanoparticles with Strong Light Absorption.
Authors: Tamarov, Konstantin and Wang, Julie Tzu-Wen and Kari, Juuso and Happonen, Emilia and Vesavaara, Ilkka and Niemelä, Matti and Perämäki, Paavo and Al-Jamal, Khuloud T and Xu, Wujun and Lehto, Vesa-Pekka
Journal: ACS applied materials & interfaces (2021): 40392-40400
Authors: Tamarov, Konstantin and Wang, Julie Tzu-Wen and Kari, Juuso and Happonen, Emilia and Vesavaara, Ilkka and Niemelä, Matti and Perämäki, Paavo and Al-Jamal, Khuloud T and Xu, Wujun and Lehto, Vesa-Pekka
Journal: ACS applied materials & interfaces (2021): 40392-40400
Dual contrast agents for fluorescence and photoacoustic imaging: evaluation in a murine model of prostate cancer.
Authors: Lesniak, Wojciech G and Wu, Yixuan and Kang, Jeeun and Boinapally, Srikanth and Ray Banerjee, Sangeeta and Lisok, Ala and Jablonska, Anna and Boctor, Emad M and Pomper, Martin G
Journal: Nanoscale (2021): 9217-9228
Authors: Lesniak, Wojciech G and Wu, Yixuan and Kang, Jeeun and Boinapally, Srikanth and Ray Banerjee, Sangeeta and Lisok, Ala and Jablonska, Anna and Boctor, Emad M and Pomper, Martin G
Journal: Nanoscale (2021): 9217-9228
A Targeted Activatable NIR-IIb Nanoprobe for Highly Sensitive Detection of Ischemic Stroke in a Photothrombotic Stroke Model.
Authors: Yang, Xiaohu and Wang, Zan and Huang, Haoying and Ling, Sisi and Zhang, Rong and Zhang, Yejun and Chen, Guangcun and Li, Chunyan and Wang, Qiangbin
Journal: Advanced healthcare materials (2021): e2001544
Authors: Yang, Xiaohu and Wang, Zan and Huang, Haoying and Ling, Sisi and Zhang, Rong and Zhang, Yejun and Chen, Guangcun and Li, Chunyan and Wang, Qiangbin
Journal: Advanced healthcare materials (2021): e2001544
Design of Fluorescence-Enhanced Silver Nanoisland Chips for High-Throughput and Rapid Arsenite Assay.
Authors: Yen, Hung-Chi and Kuo, Tsung-Rong and Huang, Min-Hui and Huang, Hao-Kang and Chen, Chia-Chun
Journal: ACS omega (2020): 19771-19777
Authors: Yen, Hung-Chi and Kuo, Tsung-Rong and Huang, Min-Hui and Huang, Hao-Kang and Chen, Chia-Chun
Journal: ACS omega (2020): 19771-19777