Cyanine 7.5 monoacid [equivalent to Cy7.5® acid]
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1057.10 |
Solvent | DMSO |
Spectral properties
Extinction coefficient (cm -1 M -1) | 250000 |
Excitation (nm) | 785 |
Emission (nm) | 801 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
Molecular weight 1057.10 | Extinction coefficient (cm -1 M -1) 250000 | Excitation (nm) 785 | Emission (nm) 801 |
Various cyanine dyes have been extensively used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analyses. These dyes are commonly utilized for labeling peptides, proteins, and oligos. Among them, cyanine 7.5 (Cy7.5) is a notable near-infrared dye with an exceptionally long-wave emission. Its pH insensitivity over a wide molar range (pH 3-10), makes it suitable for an extensive range of applications at biologically relevant pH levels. Moreover, the NIR emission of Cy7.5 enables deep tissue penetration, making it ideal for in vivo imaging. These dyes are DMSO-tolerant and photostable, ensuring that they can be transferred from storage to assay without any loss of performance. Our Cy7.5 dyes undergo comprehensive quality control testing to ensure high levels of chromophore and reactive dye content. For labeling reactions, mono-reactive dyes are ideal for targeted, precise labeling of proteins and oligonucleotides, while bis-reactive dyes are more suitable for general protein labeling. NHS ester dyes are recommended for labeling amine groups, and maleimide dyes are suggested for labeling thiol groups.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Cyanine 7.5 monoacid [equivalent to Cy7.5® acid] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 94.598 µL | 472.992 µL | 945.984 µL | 4.73 mL | 9.46 mL |
5 mM | 18.92 µL | 94.598 µL | 189.197 µL | 945.984 µL | 1.892 mL |
10 mM | 9.46 µL | 47.299 µL | 94.598 µL | 472.992 µL | 945.984 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Spectrum
Open in Advanced Spectrum Viewer
![spectrum](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fspectra%2Fcy7_5_cyanine_7_5_.png&w=2048&q=50)
![spectrum](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fspectra%2Fcy7_5_cyanine_7_5_.png&w=2048&q=50)
Spectral properties
Extinction coefficient (cm -1 M -1) | 250000 |
Excitation (nm) | 785 |
Emission (nm) | 801 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) | Correction Factor (482 nm) | Correction Factor (565 nm) | Correction Factor (650 nm) |
Cyanine 5.5 monoacid [equivalent to Cy5.5® acid] | 683 | 703 | 250000 | 0.27 | 0.05 | 0.101 | 0.0017 | 0.047 | 0.454 |
Images
References
View all 17 references: Citation Explorer
An NIR-IIb emissive transmembrane voltage nano-indicator for the optical monitoring of electrophysiological activities in vivo.
Authors: Xing, Zhenyu and Hu, Qian and Wang, Weikan and Kong, Na and Gao, Rong and Shen, Xiaolei and Xu, Sixin and Meng, Lingkai and Liu, Jian-Ren and Zhu, Xingjun
Journal: Materials horizons (2024)
Authors: Xing, Zhenyu and Hu, Qian and Wang, Weikan and Kong, Na and Gao, Rong and Shen, Xiaolei and Xu, Sixin and Meng, Lingkai and Liu, Jian-Ren and Zhu, Xingjun
Journal: Materials horizons (2024)
Theoretical Study on Spectrum and Luminescence Mechanism of Cy5.5 and Cy7.5 Dye Based on Density Functional Theory (DFT).
Authors: Liu, Zhao and Meng, Xv and Zhang, Zhengze and Liu, Runzhang and Wang, Shutao and Lei, Jun-Qiang
Journal: Journal of fluorescence (2023)
Authors: Liu, Zhao and Meng, Xv and Zhang, Zhengze and Liu, Runzhang and Wang, Shutao and Lei, Jun-Qiang
Journal: Journal of fluorescence (2023)
PSMA-targeted NIR probes for image-guided detection of prostate cancer.
Authors: Cilibrizzi, Agostino and Wang, Julie Tzu-Wen and Memdouh, Siham and Iacovone, Antonella and McElroy, Kate and Jaffar, Noor and Young, Jennifer Denise and Hider, Robert C and Blower, Philip and Al-Jamal, Khuloud and Abbate, Vincenzo
Journal: Colloids and surfaces. B, Biointerfaces (2022): 112734
Authors: Cilibrizzi, Agostino and Wang, Julie Tzu-Wen and Memdouh, Siham and Iacovone, Antonella and McElroy, Kate and Jaffar, Noor and Young, Jennifer Denise and Hider, Robert C and Blower, Philip and Al-Jamal, Khuloud and Abbate, Vincenzo
Journal: Colloids and surfaces. B, Biointerfaces (2022): 112734
Dual tumor- and subcellular-targeted photodynamic therapy using glucose-functionalized MoS2 nanoflakes for multidrug-resistant tumor ablation.
Authors: Xu, Shaohui and Zhang, Pan and Heing-Becker, Isabelle and Zhang, Junmei and Tang, Peng and Bej, Raju and Bhatia, Sumati and Zhong, Yinan and Haag, Rainer
Journal: Biomaterials (2022): 121844
Authors: Xu, Shaohui and Zhang, Pan and Heing-Becker, Isabelle and Zhang, Junmei and Tang, Peng and Bej, Raju and Bhatia, Sumati and Zhong, Yinan and Haag, Rainer
Journal: Biomaterials (2022): 121844
Tracking the gastrointestinal digestive and metabolic behaviour of Dendrobium officinale polysaccharides by fluorescent labelling.
Authors: Wu, Zhijing and Zhang, Yu and Nie, Gang and Liu, Junxi and Mei, Hao and He, Zihao and Dou, Pengfei and Wang, Kaiping
Journal: Food & function (2022): 7274-7286
Authors: Wu, Zhijing and Zhang, Yu and Nie, Gang and Liu, Junxi and Mei, Hao and He, Zihao and Dou, Pengfei and Wang, Kaiping
Journal: Food & function (2022): 7274-7286
A Targeted Activatable NIR-IIb Nanoprobe for Highly Sensitive Detection of Ischemic Stroke in a Photothrombotic Stroke Model.
Authors: Yang, Xiaohu and Wang, Zan and Huang, Haoying and Ling, Sisi and Zhang, Rong and Zhang, Yejun and Chen, Guangcun and Li, Chunyan and Wang, Qiangbin
Journal: Advanced healthcare materials (2021): e2001544
Authors: Yang, Xiaohu and Wang, Zan and Huang, Haoying and Ling, Sisi and Zhang, Rong and Zhang, Yejun and Chen, Guangcun and Li, Chunyan and Wang, Qiangbin
Journal: Advanced healthcare materials (2021): e2001544
Bimodal Imaging-Visible Nanomedicine Integrating CXCR4 and VEGFa Genes Directs Synergistic Reendothelialization of Endothelial Progenitor Cells.
Authors: Yu, Bingbo and Dong, Bing and He, Jiang and Huang, Hui and Huang, Jinsheng and Wang, Yong and Liang, Jianwen and Zhang, Jianning and Qiu, Yumin and Shen, Jun and Shuai, Xintao and Tao, Jun and Xia, Wenhao
Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany) (2020): 2001657
Authors: Yu, Bingbo and Dong, Bing and He, Jiang and Huang, Hui and Huang, Jinsheng and Wang, Yong and Liang, Jianwen and Zhang, Jianning and Qiu, Yumin and Shen, Jun and Shuai, Xintao and Tao, Jun and Xia, Wenhao
Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany) (2020): 2001657
In Vivo High-resolution Ratiometric Fluorescence Imaging of Inflammation Using NIR-II Nanoprobes with 1550 nm Emission.
Authors: Wang, Shangfeng and Liu, Lu and Fan, Yong and El-Toni, Ahmed Mohamed and Alhoshan, Mansour Saleh and Li, Dandan and Zhang, Fan
Journal: Nano letters (2019): 2418-2427
Authors: Wang, Shangfeng and Liu, Lu and Fan, Yong and El-Toni, Ahmed Mohamed and Alhoshan, Mansour Saleh and Li, Dandan and Zhang, Fan
Journal: Nano letters (2019): 2418-2427
Quick-Responsive Polymer-Based Thermosensitive Liposomes for Controlled Doxorubicin Release and Chemotherapy.
Authors: Mo, Yulin and Du, Hongliang and Chen, Binlong and Liu, Dechun and Yin, Qingqing and Yan, Yue and Wang, Zenghui and Wan, Fangjie and Qi, Tong and Wang, Yaoqi and Zhang, Qiang and Wang, Yiguang
Journal: ACS biomaterials science & engineering (2019): 2316-2329
Authors: Mo, Yulin and Du, Hongliang and Chen, Binlong and Liu, Dechun and Yin, Qingqing and Yan, Yue and Wang, Zenghui and Wan, Fangjie and Qi, Tong and Wang, Yaoqi and Zhang, Qiang and Wang, Yiguang
Journal: ACS biomaterials science & engineering (2019): 2316-2329
A dual-targeted hyaluronic acid-gold nanorod platform with triple-stimuli responsiveness for photodynamic/photothermal therapy of breast cancer.
Authors: Xu, Weijun and Qian, Junmin and Hou, Guanghui and Wang, Yaping and Wang, Jinlei and Sun, Tiantian and Ji, Lijie and Suo, Aili and Yao, Yu
Journal: Acta biomaterialia (2019): 400-413
Authors: Xu, Weijun and Qian, Junmin and Hou, Guanghui and Wang, Yaping and Wang, Jinlei and Sun, Tiantian and Ji, Lijie and Suo, Aili and Yao, Yu
Journal: Acta biomaterialia (2019): 400-413