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Cyanine 7.5 monoacid [equivalent to Cy7.5® acid]

Various cyanine dyes have been extensively used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analyses. These dyes are commonly utilized for labeling peptides, proteins, and oligos. Among them, cyanine 7.5 (Cy7.5) is a notable near-infrared dye with an exceptionally long-wave emission. Its pH insensitivity over a wide molar range (pH 3-10), makes it suitable for an extensive range of applications at biologically relevant pH levels. Moreover, the NIR emission of Cy7.5 enables deep tissue penetration, making it ideal for in vivo imaging. These dyes are DMSO-tolerant and photostable, ensuring that they can be transferred from storage to assay without any loss of performance. Our Cy7.5 dyes undergo comprehensive quality control testing to ensure high levels of chromophore and reactive dye content. For labeling reactions, mono-reactive dyes are ideal for targeted, precise labeling of proteins and oligonucleotides, while bis-reactive dyes are more suitable for general protein labeling. NHS ester dyes are recommended for labeling amine groups, and maleimide dyes are suggested for labeling thiol groups.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)Correction Factor (482 nm)Correction Factor (565 nm)Correction Factor (650 nm)
Cyanine 5.5 monoacid [equivalent to Cy5.5® acid]6837032500000.270.050.1010.00170.0470.454

References

View all 17 references: Citation Explorer
An NIR-IIb emissive transmembrane voltage nano-indicator for the optical monitoring of electrophysiological activities in vivo.
Authors: Xing, Zhenyu and Hu, Qian and Wang, Weikan and Kong, Na and Gao, Rong and Shen, Xiaolei and Xu, Sixin and Meng, Lingkai and Liu, Jian-Ren and Zhu, Xingjun
Journal: Materials horizons (2024)
Theoretical Study on Spectrum and Luminescence Mechanism of Cy5.5 and Cy7.5 Dye Based on Density Functional Theory (DFT).
Authors: Liu, Zhao and Meng, Xv and Zhang, Zhengze and Liu, Runzhang and Wang, Shutao and Lei, Jun-Qiang
Journal: Journal of fluorescence (2023)
PSMA-targeted NIR probes for image-guided detection of prostate cancer.
Authors: Cilibrizzi, Agostino and Wang, Julie Tzu-Wen and Memdouh, Siham and Iacovone, Antonella and McElroy, Kate and Jaffar, Noor and Young, Jennifer Denise and Hider, Robert C and Blower, Philip and Al-Jamal, Khuloud and Abbate, Vincenzo
Journal: Colloids and surfaces. B, Biointerfaces (2022): 112734
Dual tumor- and subcellular-targeted photodynamic therapy using glucose-functionalized MoS2 nanoflakes for multidrug-resistant tumor ablation.
Authors: Xu, Shaohui and Zhang, Pan and Heing-Becker, Isabelle and Zhang, Junmei and Tang, Peng and Bej, Raju and Bhatia, Sumati and Zhong, Yinan and Haag, Rainer
Journal: Biomaterials (2022): 121844
Tracking the gastrointestinal digestive and metabolic behaviour of Dendrobium officinale polysaccharides by fluorescent labelling.
Authors: Wu, Zhijing and Zhang, Yu and Nie, Gang and Liu, Junxi and Mei, Hao and He, Zihao and Dou, Pengfei and Wang, Kaiping
Journal: Food & function (2022): 7274-7286
Page updated on November 21, 2024

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Physical properties

Molecular weight

1057.10

Solvent

DMSO

Spectral properties

Extinction coefficient (cm -1 M -1)

250000

Excitation (nm)

785

Emission (nm)

801

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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