Cy5 Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*
A Cy5 dye qPCR calibration plate is a type of plate used in quantitative PCR (polymerase chain reaction) experiments. It is designed to calibrate the real-time PCR instrument, which measures the fluorescence signal emitted by the Cy5 dye during the PCR reaction. The Cy5 dye qPCR calibration plate contains a set of pre-determined concentrations of a DNA template that serves as a reference for the quantification of unknown samples. The plate is loaded into the PCR instrument along with the unknown samples, and the instrument measures the fluorescence signal from each well during the PCR amplification process. By comparing the fluorescence signal of the unknown samples to the known concentrations in the calibration plate, researchers can determine the amount of DNA present in the unknown samples. This information can be used to quantify gene expression levels, detect the presence of pathogens, or perform other types of molecular analysis.
Spectrum
Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
Cy5 Dye qPCR Calibration Solution *10,000X* | 651 | 670 | 2500001 |
TAMRA Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 552 | 578 | 90000 |
VIC Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 526 | 543 | - |
ROX Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 578 | 604 | 82000 |
FAM Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 493 | 517 | 83000 |
SYBR Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 498 | 522 | - |
NED Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 545 | 567 | 740001 |
JOE Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 520 | 545 | 750001 |
ABY Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 559 | 570 | 1200001 |
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References
Authors: Lothert, Keven and Bagrin, Elena and Wolff, Michael W
Journal: Viruses (2023)
Authors: Rioboó-Legaspi, P and González-López, A and Beltrán-Sánchez, J F and Cima-Cabal, M D and García-Suárez, M M and Sánchez, A J García and Fernández-Otero, T and Haro, J García and Costa-Rama, E and Fernández-Abedul, M T
Journal: Talanta (2023): 124963
Authors: Lin, Yingying and Lai, Xiaofan and Huang, Shaojie and Pu, Lvya and Zeng, Qihao and Wang, Zhongxing and Huang, Wenqi
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Authors: Betts, Kim S and Marathe, Supreet P and Chai, Kevin and Konstantinov, Igor and Iyengar, Ajay and Suna, Jessica and , and Venugopal, Prem and Alphonso, Nelson
Journal: European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery (2023)
Authors: Zhao, Bin and Fang, Fang and Liao, Yiqun and Chen, Yuji and Wang, Fei and Ma, Yichao and Wei, Chen and Zhao, Jiahao and Ji, Hao and Wang, Daorong and Tang, Dong
Journal: BMC bioinformatics (2023): 100