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Cy5 Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Spectral properties
| Correction Factor (260 nm) | 0.02 |
| Correction Factor (280 nm) | 0.03 |
| Correction Factor (482 nm) | 0.009 |
| Correction Factor (565 nm) | 0.09 |
| Extinction coefficient (cm -1 M -1) | 2500001 |
| Excitation (nm) | 651 |
| Emission (nm) | 670 |
| Quantum yield | 0.271, 0.42 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| Overview |  SDSProtocol |
See also: Real-Time PCR (qPCR)
Correction Factor (260 nm) 0.02 | Correction Factor (280 nm) 0.03 | Correction Factor (482 nm) 0.009 | Correction Factor (565 nm) 0.09 | Extinction coefficient (cm -1 M -1) 2500001 | Excitation (nm) 651 | Emission (nm) 670 | Quantum yield 0.271, 0.42 |
A Cy5 dye qPCR calibration plate is a type of plate used in quantitative PCR (polymerase chain reaction) experiments. It is designed to calibrate the real-time PCR instrument, which measures the fluorescence signal emitted by the Cy5 dye during the PCR reaction. The Cy5 dye qPCR calibration plate contains a set of pre-determined concentrations of a DNA template that serves as a reference for the quantification of unknown samples. The plate is loaded into the PCR instrument along with the unknown samples, and the instrument measures the fluorescence signal from each well during the PCR amplification process. By comparing the fluorescence signal of the unknown samples to the known concentrations in the calibration plate, researchers can determine the amount of DNA present in the unknown samples. This information can be used to quantify gene expression levels, detect the presence of pathogens, or perform other types of molecular analysis.
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (260 nm) | 0.02 |
| Correction Factor (280 nm) | 0.03 |
| Correction Factor (482 nm) | 0.009 |
| Correction Factor (565 nm) | 0.09 |
| Extinction coefficient (cm -1 M -1) | 2500001 |
| Excitation (nm) | 651 |
| Emission (nm) | 670 |
| Quantum yield | 0.271, 0.42 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| Cy5 Dye qPCR Calibration Solution *10,000X* | 651 | 670 | 2500001 |
| TAMRA Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 552 | 578 | 90000 |
| VIC Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 526 | 543 | - |
| ROX Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 578 | 604 | 82000 |
| FAM Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 493 | 517 | 83000 |
| SYBR Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 498 | 522 | - |
| NED Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 545 | 567 | 740001 |
| JOE Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 520 | 545 | 750001 |
| ABY Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 559 | 570 | 1200001 |
Show More (2) | |||
References
View all 50 references: Citation Explorer
Evaluating Novel Quantification Methods for Infectious Baculoviruses.
Authors: Lothert, Keven and Bagrin, Elena and Wolff, Michael W
Journal: Viruses (2023)
Authors: Lothert, Keven and Bagrin, Elena and Wolff, Michael W
Journal: Viruses (2023)
Phenol red as electrochemical indicator for highly sensitive quantification of SARS-CoV-2 by loop-mediated isothermal amplification detection.
Authors: Rioboó-Legaspi, P and González-López, A and Beltrán-Sánchez, J F and Cima-Cabal, M D and García-Suárez, M M and Sánchez, A J García and Fernández-Otero, T and Haro, J García and Costa-Rama, E and Fernández-Abedul, M T
Journal: Talanta (2023): 124963
Authors: Rioboó-Legaspi, P and González-López, A and Beltrán-Sánchez, J F and Cima-Cabal, M D and García-Suárez, M M and Sánchez, A J García and Fernández-Otero, T and Haro, J García and Costa-Rama, E and Fernández-Abedul, M T
Journal: Talanta (2023): 124963
Identification of diagnostic hub genes related to neutrophils and infiltrating immune cell alterations in idiopathic pulmonary fibrosis.
Authors: Lin, Yingying and Lai, Xiaofan and Huang, Shaojie and Pu, Lvya and Zeng, Qihao and Wang, Zhongxing and Huang, Wenqi
Journal: Frontiers in immunology (2023): 1078055
Authors: Lin, Yingying and Lai, Xiaofan and Huang, Shaojie and Pu, Lvya and Zeng, Qihao and Wang, Zhongxing and Huang, Wenqi
Journal: Frontiers in immunology (2023): 1078055
A machine learning approach to predicting 30-day mortality following paediatric cardiac surgery: findings from the Australia New Zealand Congenital Outcomes Registry for Surgery (ANZCORS).
Authors: Betts, Kim S and Marathe, Supreet P and Chai, Kevin and Konstantinov, Igor and Iyengar, Ajay and Suna, Jessica and , and Venugopal, Prem and Alphonso, Nelson
Journal: European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery (2023)
Authors: Betts, Kim S and Marathe, Supreet P and Chai, Kevin and Konstantinov, Igor and Iyengar, Ajay and Suna, Jessica and , and Venugopal, Prem and Alphonso, Nelson
Journal: European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery (2023)
Novel m7G-related lncRNA signature for predicting overall survival in patients with gastric cancer.
Authors: Zhao, Bin and Fang, Fang and Liao, Yiqun and Chen, Yuji and Wang, Fei and Ma, Yichao and Wei, Chen and Zhao, Jiahao and Ji, Hao and Wang, Daorong and Tang, Dong
Journal: BMC bioinformatics (2023): 100
Authors: Zhao, Bin and Fang, Fang and Liao, Yiqun and Chen, Yuji and Wang, Fei and Ma, Yichao and Wei, Chen and Zhao, Jiahao and Ji, Hao and Wang, Daorong and Tang, Dong
Journal: BMC bioinformatics (2023): 100
A comparison of qPCR and microscopy for the detection and enumeration of Cryptosporidium oocysts from drinking water.
Authors: Robinson, Guy and Elwin, Kristin and Jones, Matthew and Chalmers, Rachel M
Journal: Journal of medical microbiology (2023)
Authors: Robinson, Guy and Elwin, Kristin and Jones, Matthew and Chalmers, Rachel M
Journal: Journal of medical microbiology (2023)
CCT3 as a Diagnostic and Prognostic Biomarker in Cervical Cancer.
Authors: Li, Man and Zeng, Jianmin and Chang, Yuhuan and Lv, Lili and Ye, Guoliu
Journal: Critical reviews in eukaryotic gene expression (2023): 17-28
Authors: Li, Man and Zeng, Jianmin and Chang, Yuhuan and Lv, Lili and Ye, Guoliu
Journal: Critical reviews in eukaryotic gene expression (2023): 17-28
Specific detection of Treponema pallidum in clinical samples: validation of a qPCR assay combining two genomic targets.
Authors: Salle, Romain and Mayslich, Constance and Grange, Philippe Alain and Leducq, Valentin and Ollagnier, Guillaume and Heller, Ugo and Saule, Julie and Martinet, Pervenche and Robert, Jean-Luc and Benhaddou, Nadjet and Fouere, Sebastien and Dupin, Nicolas
Journal: Sexually transmitted infections (2023): 91-96
Authors: Salle, Romain and Mayslich, Constance and Grange, Philippe Alain and Leducq, Valentin and Ollagnier, Guillaume and Heller, Ugo and Saule, Julie and Martinet, Pervenche and Robert, Jean-Luc and Benhaddou, Nadjet and Fouere, Sebastien and Dupin, Nicolas
Journal: Sexually transmitted infections (2023): 91-96
Integrated bioinformatics analysis for conducting a prognostic model and identifying immunotherapeutic targets in gastric cancer.
Authors: Liu, YaLing and Li, Dan and Chen, Yong and Liu, YiJuan and Lin, YiJuan and Huang, XunRu and Wu, Ting and Wang, ChengDang and Ding, Jian
Journal: BMC bioinformatics (2023): 191
Authors: Liu, YaLing and Li, Dan and Chen, Yong and Liu, YiJuan and Lin, YiJuan and Huang, XunRu and Wu, Ting and Wang, ChengDang and Ding, Jian
Journal: BMC bioinformatics (2023): 191
STAMP-Based Digital CRISPR-Cas13a for Amplification-Free Quantification of HIV-1 Plasma Viral Loads.
Authors: Nouri, Reza and Jiang, Yuqian and Politza, Anthony J and Liu, Tianyi and Greene, Wallace H and Zhu, Yusheng and Nunez, Jonathan J and Lian, Xiaojun and Guan, Weihua
Journal: ACS nano (2023): 10701-10712
Authors: Nouri, Reza and Jiang, Yuqian and Politza, Anthony J and Liu, Tianyi and Greene, Wallace H and Zhu, Yusheng and Nunez, Jonathan J and Lian, Xiaojun and Guan, Weihua
Journal: ACS nano (2023): 10701-10712
Application notes
FAQ
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Are mRNAs found in prokaryotes differ from those of eukaryotes?
Are spliceosomes associated with any diseases?
Are there any alternatives for ethidium bromide in agarose gels?
Are there any alternatives to Cy5?
Are mRNAs found in prokaryotes differ from those of eukaryotes?
Are spliceosomes associated with any diseases?
Are there any alternatives for ethidium bromide in agarose gels?
Are there any alternatives to Cy5?