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CUG [3-Carboxyumbelliferyl beta-D-galactopyranoside] *CAS 64664-99-9*
CUG is a fluorogenic galactosidase substrate with carboxy reactive handle that can be used for conjugation. The organic and inorganic esters and glycosides of 7-hydroxycoumarin are among the most commonly used fluorogenic substrates. These substrates yield water-soluble blue fluorescent coumarin fluorophores upon interaction with enzymes.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of CUG [3-Carboxyumbelliferyl beta-D-galactopyranoside] *CAS 64664-99-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 271.525 µL | 1.358 mL | 2.715 mL | 13.576 mL | 27.153 mL |
| 5 mM | 54.305 µL | 271.525 µL | 543.05 µL | 2.715 mL | 5.431 mL |
| 10 mM | 27.153 µL | 135.763 µL | 271.525 µL | 1.358 mL | 2.715 mL |
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References
View all 9 references: Citation Explorer
The translation of an antiapoptotic protein HIAP2 is regulated by an upstream open reading frame
Authors: Warnakulasuriyarachchi D, Ungureanu NH, Holcik M.
Journal: Cell Death Differ (2003): 899
Authors: Warnakulasuriyarachchi D, Ungureanu NH, Holcik M.
Journal: Cell Death Differ (2003): 899
Absence of effect of varying Thr-Leu codon pairs on protein synthesis in a T7 system
Authors: Cheng L, Goldman E.
Journal: Biochemistry (2001): 6102
Authors: Cheng L, Goldman E.
Journal: Biochemistry (2001): 6102
The CUG-binding protein binds specifically to UG dinucleotide repeats in a yeast three-hybrid system
Authors: Takahashi N, Sasagawa N, Suzuki K, Ishiura S.
Journal: Biochem Biophys Res Commun (2000): 518
Authors: Takahashi N, Sasagawa N, Suzuki K, Ishiura S.
Journal: Biochem Biophys Res Commun (2000): 518
An AUG initiation codon, not codon-anticodon complementarity, is required for the translation of unleadered mRNA in Escherichia coli
Authors: Van Etten WJ, Janssen GR.
Journal: Mol Microbiol (1998): 987
Authors: Van Etten WJ, Janssen GR.
Journal: Mol Microbiol (1998): 987
Molecular characterisation of the pifC gene encoding translation initiation factor 3, which is required for normal photosynthetic complex formation in Rhodobacter sphaeroides NCIB 8253
Authors: Babic S, Hunter CN, Rakhlin NJ, Simons RW, Phillips-Jones MK.
Journal: Eur J Biochem (1997): 564
Authors: Babic S, Hunter CN, Rakhlin NJ, Simons RW, Phillips-Jones MK.
Journal: Eur J Biochem (1997): 564
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