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Cl-NERF
Cl-NERF is a rhodol dye with pKa = 3.8. It has been mainly used for monitoring pH in acidic environments such as endosomes and lysosomes. Cl-NERF has a ratiometric excitation spectrum with 510/450 nm excitation ratio commonly used. The dextran conjugates of Cl-NERF has been used for detect the uptake of labeled probes into acidic organelles.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Cl-NERF to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 221.307 µL | 1.107 mL | 2.213 mL | 11.065 mL | 22.131 mL |
| 5 mM | 44.261 µL | 221.307 µL | 442.615 µL | 2.213 mL | 4.426 mL |
| 10 mM | 22.131 µL | 110.654 µL | 221.307 µL | 1.107 mL | 2.213 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| NBD-Cl [4-Chloro-7-nitrobenzofurazan] *CAS 10199-89-0* | 467 | 539 | 22000 | - |
| CL-APC [Cross linked-AlloPhycocyanin] | 651 | 660 | 730000 | 0.195 |
References
View all 12 references: Citation Explorer
Inhibitors of acid secretion can benefit gastric wound repair independent of luminal pH effects on the site of damage
Authors: Demitrack ES, Aihara E, Kenny S, Varro A, Montrose MH.
Journal: Gut (2012): 804
Authors: Demitrack ES, Aihara E, Kenny S, Varro A, Montrose MH.
Journal: Gut (2012): 804
Damage to the gastric epithelium activates cellular bicarbonate secretion via SLC26A9 Cl(-)/HCO(3)(-)
Authors: Demitrack ES, Soleimani M, Montrose MH.
Journal: Am J Physiol Gastrointest Liver Physiol (2010): G255
Authors: Demitrack ES, Soleimani M, Montrose MH.
Journal: Am J Physiol Gastrointest Liver Physiol (2010): G255
In vivo action of trefoil factor 2 (TFF2) to speed gastric repair is independent of cyclooxygenase
Authors: Xue L, Aihara E, Podolsky DK, Wang TC, Montrose MH.
Journal: Gut (2010): 1184
Authors: Xue L, Aihara E, Podolsky DK, Wang TC, Montrose MH.
Journal: Gut (2010): 1184
Disruption of the Cox-1 gene slows repair of microscopic lesions in the mouse gastric epithelium
Authors: Starodub OT, Demitrack ES, Baumgartner HK, Montrose MH.
Journal: Am J Physiol Cell Physiol (2008): C223
Authors: Starodub OT, Demitrack ES, Baumgartner HK, Montrose MH.
Journal: Am J Physiol Cell Physiol (2008): C223
Cyclooxygenase 1 is required for pH control at the mouse gastric surface
Authors: Baumgartner HK, Starodub OT, Joehl JS, Tackett L, Montrose MH.
Journal: Gut (2004): 1751
Authors: Baumgartner HK, Starodub OT, Joehl JS, Tackett L, Montrose MH.
Journal: Gut (2004): 1751
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