logo
AAT Bioquest

CF-MUP, sodium salt *Superior alternative to MUP*

Although MUP is widely used for detecting phosphatases in solution it is not well suited for living cell or continuous assays since MU (4-methylumbelliferone), the enzymatic product, which only develops maximum fluorescence at pH value of >10. Thus it is also difficult to use MUP for the detection of phosphatases that have acidic optimal pH range such as acid phosphatases. AAT Bioquest is pleased to offer CF-MUP Plus that is developed to address this pH limitation associated with MUP substrates. CF-MU exhibits maximum fluorescence above pH 5.0, thus CF-MUP substrate can be well used for continuous phosphatase assays. It can also be used for the assays that require acidic pH such as acid phosphatases and some protein phosphatases.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of CF-MUP, sodium salt *Superior alternative to MUP* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM283.648 µL1.418 mL2.836 mL14.182 mL28.365 mL
5 mM56.73 µL283.648 µL567.295 µL2.836 mL5.673 mL
10 mM28.365 µL141.824 µL283.648 µL1.418 mL2.836 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Spectrum

References

View all 76 references: Citation Explorer
Application of intracellular alkaline phosphatase activity measurement in detection of neutrophil adherence in vitro
Authors: Bednarska K, Klink M, Sulowska Z.
Journal: Mediators Inflamm (2006): 19307
Evaluation of an alternative method for the enumeration and confirmation of Clostridium perfringens from treated and untreated sewages
Authors: Wohlsen T, Bayliss J, Gray B, Bates J, Katouli M.
Journal: Lett Appl Microbiol (2006): 438
Fluorometric cell-ELISA for quantifying rabies infection and heparin inhibition
Authors: Rincon V, Corredor A, Martinez-Gutierrez M, Castellanos JE.
Journal: J Virol Methods (2005): 33
Relative movement and soil fixation of soluble organic and inorganic phosphorus
Authors: Anderson BH, Magdoff FR.
Journal: J Environ Qual (2005): 2228
Purification and partial characterization of an acid phosphatase from Spirodela oligorrhiza and its affinity for selected organophosphate pesticides
Authors: Hoehamer CF, Mazur CS, Wolfe NL.
Journal: J Agric Food Chem (2005): 90
Page updated on July 12, 2023

Ordering information

Price
Unit size
Catalog Number11628
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Request quotation

Physical properties

Molecular weight

352.55

Solvent

Water

Spectral properties

Excitation (nm)

356

Emission (nm)

456

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Detection of acid phosphatase activity CF-MUP and MUP. The concentration of the two substrates (initially approximately 10 uM) were matched by normalizing the obsorbance at 319 nm (pH = 10) to a value of 0.52 (assuming the extinction coefficient of each substrate was approximately equivalent). The resulting fluorescence signal was recorded using Ex/Em = 360/450 nm.
Detection of acid phosphatase activity CF-MUP and MUP. The concentration of the two substrates (initially approximately 10 uM) were matched by normalizing the obsorbance at 319 nm (pH = 10) to a value of 0.52 (assuming the extinction coefficient of each substrate was approximately equivalent). The resulting fluorescence signal was recorded using Ex/Em = 360/450 nm.
Detection of acid phosphatase activity CF-MUP and MUP. The concentration of the two substrates (initially approximately 10 uM) were matched by normalizing the obsorbance at 319 nm (pH = 10) to a value of 0.52 (assuming the extinction coefficient of each substrate was approximately equivalent). The resulting fluorescence signal was recorded using Ex/Em = 360/450 nm.