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CF-MU [6-Chloro-8-fluoro-umbelliferone] *Fluorescence reference standard*
This compound is used as a calibration standard for CF-MU-based enzyme substrates. It has pH-dependent fluorescence. Compared to umbelliferone, this dye has much lower pKa, making it more fluorescent than umbelliferone under physiological conditions.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of CF-MU [6-Chloro-8-fluoro-umbelliferone] *Fluorescence reference standard* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 437.445 µL | 2.187 mL | 4.374 mL | 21.872 mL | 43.745 mL |
| 5 mM | 87.489 µL | 437.445 µL | 874.891 µL | 4.374 mL | 8.749 mL |
| 10 mM | 43.745 µL | 218.723 µL | 437.445 µL | 2.187 mL | 4.374 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
References
View all 10 references: Citation Explorer
A Novel Fluorogenic Coumarin Substrate for Monitoring Acid Phosphatase Activity at Low pH Environment
Authors: Yang D, Z Li, Y Diwu, H Fu, J Liao, C Wei, and Z Diwu, undefined
Journal: Curr Chem Genomics (2008): 48–50
Authors: Yang D, Z Li, Y Diwu, H Fu, J Liao, C Wei, and Z Diwu, undefined
Journal: Curr Chem Genomics (2008): 48–50
Novel thiocoumarins as inhibitors of TNF-alpha induced ICAM-1 expression on human umbilical vein endothelial cells (HUVECs) and microsomal lipid peroxidation
Authors: Kumar S, Singh BK, Kalra N, Kumar V, Kumar A, Prasad AK, Raj HG, Parmar VS, Ghosh B.
Journal: Bioorg Med Chem (2005): 1605
Authors: Kumar S, Singh BK, Kalra N, Kumar V, Kumar A, Prasad AK, Raj HG, Parmar VS, Ghosh B.
Journal: Bioorg Med Chem (2005): 1605
Excited state properties of 7-hydroxy-4-methylcoumarin in the gas phase and in solution. A theoretical study
Authors: Georgieva I, Trendafilova N, Aquino A, Lischka H.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2005): 11860
Authors: Georgieva I, Trendafilova N, Aquino A, Lischka H.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2005): 11860
Measurement of enzyme kinetics using microscale steady-state kinetic analysis
Authors: Gleason NJ, Carbeck JD.
Journal: Langmuir (2004): 6374
Authors: Gleason NJ, Carbeck JD.
Journal: Langmuir (2004): 6374
Stability of the complexes of some lanthanides with coumarin derivatives. I. Cerium(III)-4-methyl-7-hydroxycoumarin
Authors: Kostova IP, Manolov, II, Radulova MK.
Journal: Acta Pharm (2004): 37
Authors: Kostova IP, Manolov, II, Radulova MK.
Journal: Acta Pharm (2004): 37
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