Cell Navigator® TMR Ceramide Golgi Staining Kit *Red Fluorescence*
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Solvent | DMSO |
Excitation (nm) | 544 |
Emission (nm) | 570 |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
Excitation (nm) 544 | Emission (nm) 570 |
Platform
Fluorescence microscope
Excitation | Cy3/TRITC filter set |
Emission | Cy3/TRITC filter set |
Recommended plate | Black wall/clear bottom |
Components
Example protocol
AT A GLANCE
- Treat cells as desired
- Add GGR169-ceramide working solution and incubate at room temperature or 37 °C for 15∼30 minutes
- Replace with the Staining Buffer
- Observe under microscope using Cy3 filter set
Thaw all the components at room temperature before starting the experiment.
CELL PREPARATION
For guidelines on cell sample preparation, please visit https://www.aatbio.com/resources/guides/cell-sample-preparation.html
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles
Add 100 µL of DMSO (Component C) to GGR-ceramide (Component A) to make GGR169-ceramide stock solution (100X).
Note Store the unused GGR-ceramide stock solution at -20 °C in single use aliquots to avoid freeze thaw cycles.
PREPARATION OF WORKING SOLUTION
Add 10 µL of GGR169-ceramide stock solution (100X) to 990 µL of Staining Buffer (Component B) to make GGR169-ceramide working solution.
Optional: Add 10 µL of Hoechst 33342 (Component D) to 1 mL GGR169-ceramide working solution for nuclear stain. Observe under fluorescence microscope with DAPI filter set.
SAMPLE EXPERIMENTAL PROTOCOL
Following protocol should be used for the guidelines and can be modified as per requirements.
- Plate and treat cells as desired.
Remove the cell culture medium. Optional: Cells can be washed with buffer of your choice.
Add 100 µL of GGR169-ceramide working solution directly into cell culture medium.
Incubate at room temperature or 37 °C for 15∼30 minutes.
Remove the GGR169-ceramide working solution and wash once with DPBS or buffer of your choice.
Add 100 µL/well of Staining Buffer (Component B).
Observe under a fluorescence microscope with Cy3 filter set.
Product Family
Name | Excitation (nm) | Emission (nm) |
Cell Navigator® NBD Ceramide Golgi Staining Kit *Green Fluorescence* | 467 | 538 |
Images
Citations
Authors: Sasaki, Izumi and Fukuda-Ohta, Yuri and Nakai, Chihiro and Wakaki-Nishiyama, Naoko and Okamoto, Chizuyo and Okuzaki, Daisuke and Morita, Shuhei and Kaji, Shiori and Furuta, Yuki and Hemmi, Hiroaki and others,
Journal: Cell Reports (2024)
References
Authors: Zelnik, Iris D and Ventura, Ana E and Kim, Jiyoon L and Silva, Liana C and Futerman, Anthony H
Journal: Biochimica et biophysica acta. Molecular and cell biology of lipids (2020): 158489
Authors: Tachida, Yuriko and Kumagai, Keigo and Sakai, Shota and Ando, Shuji and Yamaji, Toshiyuki and Hanada, Kentaro
Journal: FEBS letters (2020): 519-529
Authors: Kumagai, Keigo and Hanada, Kentaro
Journal: FEBS letters (2019): 2366-2377
Authors: Adhikari, Babita and De Silva, Bhagya and Molina, Joshua A and Allen, Ashton and Peck, Sun H and Lee, Stella Y
Journal: Biochimica et biophysica acta. Molecular basis of disease (2019): 322-328
Authors: Kumagai, Keigo and Elwell, Cherilyn A and Ando, Shuji and Engel, Joanne N and Hanada, Kentaro
Journal: Biochemical and biophysical research communications (2018): 1070-1076
Authors: Back, Moon Jung and Ha, Hae Chan and Fu, Zhicheng and Choi, Jong Min and Piao, Yongwei and Won, Jong Hoon and Jang, Ji Min and Shin, In Chul and Kim, Dae Kyong
Journal: Cell death & disease (2018): 670
Authors: Giordano, Giulia
Journal: Royal Society open science (2018): 180494
Authors: Gulbins, Anne and Schumacher, Fabian and Becker, Katrin Anne and Wilker, Barbara and Soddemann, Matthias and Boldrin, Francesco and Müller, Christian P and Edwards, Michael J and Goodman, Michael and Caldwell, Charles C and Kleuser, Burkhard and Kornhuber, Johannes and Szabo, Ildiko and Gulbins, Erich
Journal: Molecular psychiatry (2018): 2324-2346
Authors: Bandet, Cécile L and Mahfouz, Rana and Véret, Julien and Sotiropoulos, Athanassia and Poirier, Maxime and Giussani, Paola and Campana, Mélanie and Philippe, Erwann and Blachnio-Zabielska, Agnieszka and Ballaire, Raphaëlle and Le Liepvre, Xavier and Bourron, Olivier and Berkeš, Dušan and Górski, Jan and Ferré, Pascal and Le Stunff, Hervé and Foufelle, Fabienne and Hajduch, Eric
Journal: Diabetes (2018): 1258-1271
Authors: Sugiki, Toshihiko and Egawa, Daichi and Kumagai, Keigo and Kojima, Chojiro and Fujiwara, Toshimichi and Takeuchi, Koh and Shimada, Ichio and Hanada, Kentaro and Takahashi, Hideo
Journal: The Journal of biological chemistry (2018): 11206-11217
Application notes
FAQ
What are the similarities between plant cells and animal cells?
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Are mRNAs found in prokaryotes differ from those of eukaryotes?
Are spliceosomes associated with any diseases?