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Product key features
Droplite™ Blue is a lipophilic stain that can specifically stain lipid droplets/adiposomes present in the cells.
- Minimal background: High specificity for lipid droplets with minimal background noise.
- Versatile applications: Compatible with various techniques, including fluorescence microscopy, flow cytometry, and fluorescence microplate readers.
- Reliable results: Superior sensitivity and specificity to lipid droplets.
- Non-toxic: Suitable for live cell imaging, offering a non-toxic method to study lipid droplets in real time.
Product description
Lipid droplets, also known as lipid bodies or adiposomes, are essential cellular organelles responsible for the storage and hydrolysis of neutral lipids. These lipid-rich structures serve as key reservoirs for fatty acids and cholesterol and play a crucial role in biological processes such as energy production, cellular signaling, and membrane maintenance. Impaired cytoplasmic lipid droplet accumulation is associated with various diseases and pathological conditions including metabolic disorders, liver dysfunction, and cardiovascular diseases.
AAT Bioquest’s Cell Navigator® Fluorimetric Lipid Droplet Assay Kit provides a powerful and reliable method for accurately studying lipid droplet accumulation within the cells. This kit features Droplite™ Blue, a lipophilic stain that exhibits bright blue fluorescence in a lipid-rich environment while giving minimal fluorescence in aqueous media. Droplite™ Blue is an ideal vital stain for visualizing intracellular lipid droplets using fluorescence microscopy, flow cytometry, or fluorescence microplate readers. The blue fluorescence can be easily detected using standard DAPI filter sets, offering a convenient and efficient tool for lipid droplet analysis.
Example protocol
AT A GLANCE
Prepare cells with test compounds.
Add Droplite™ Blue working solution.
Incubate at room temperature or 37°C for 10 to 30 minutes.
Read fluorescence intensity with a fluorescence microscope using a DAPI filter set.
This protocol is our recommended guideline for live cells, but it can be adjusted to meet your specific requirements. Since Droplite™ Blue exhibits minimal fluorescence in aqueous media, removing the growth medium and staining solution after staining is optional. Stained cells can be fixed with 3–4% formaldehyde. Alternatively, prefixed cells (fixed with 3–4% formaldehyde) can also be stained using the Droplite™ Blue staining solution.
CELL PREPARATION
For guidelines on cell sample preparation, please visit:
https://www.aatbio.com/resources/guides/cell-sample-preparation.html
PREPARATION OF WORKING SOLUTION
To prepare the Droplite™ Blue working solution, dilute 5 µL of the Droplite™ Blue (Component A) in 1 mL of Staining Buffer (Component B).
Note: 50 µL of Droplite™ Blue (Component A) is enough for one 96-well plate. Protect the solution from light. The optimal concentration of Droplite™ Blue may vary depending on the application. Adjust staining conditions based on the cell type and the permeability of cells or tissues to the probe.
SAMPLE EXPERIMENTAL PROTOCOL
Grow cells either in a 96-well black wall/clear bottom plate (100 µL/well/96-well) or on cover-slips inside a petri dish filled with the appropriate culture medium.
Gently aspirate the culture medium and add equal volume (such as 100 µL/well/96-well plate) of the Droplite™ Blue staining solution.
Incubate the cells in a 37°C, 5% CO2 incubator for 10 - 30 minutes.
Remove Droplite™ Blue working solution (Optional).
Measure fluorescence at Ex/Em = 350/450 nm with a microplate reader or observe the cells using a fluorescence microscope equipped with a DAPI filter set.
Centrifuge the cells at 1000 rpm for 5 minutes to get 1 - 5 × 105 cells per tube.
Resuspend cells in 500 µL of Droplite™ Blue working solution.
Incubate at room temperature or 37°C for 10 to 30 min, protected from light.
Centrifuge to remove the Droplite™ Blue working solution, and resuspend cells in 500 µL of pre-warmed medium or buffer of your choice to get 1 - 5 × 105 cells per tube (Optional).
Monitor the fluorescence increase using a fluorescence microscope equipped with a DAPI filter set.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| Cell Navigator® Fluorimetric Lipid Droplet Assay Kit *Green Fluorescence* | 504 | 510 | 81000 | - | 0.015 | 0.018 |
| Cell Navigator® Fluorimetric Lipid Droplet Assay Kit *Red Fluorescence* | 559 | 635 | 38000 | 0.70001 | - | - |
References
Authors: Chen, Xiuqin and Chen, Guizhi and Dong, Shiqing and Qiu, Liting and Qiu, Ruoyi and Han, Xiangyu and Wang, Zihui and Wang, Kun and Peng, Yiru
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2025): 125012
Authors: Margherita, Cortini and Elizabeta, Ilieva and Stefania, Massari and Giuliano, Bettini and Sofia, Avnet and Nicola, Baldini
Journal: Biochimica et biophysica acta. Molecular basis of disease (2025): 167576
Authors: Vardar, Umay Sevgi and Konings, Gijs and Yang, Jack and Sagis, Leonard M C and Bitter, Johannes H and Nikiforidis, Constantinos V
Journal: Journal of colloid and interface science (2025): 1077-1086
Authors: Dasso, Marina Ercilia and Centola, Cecilia Lucia and Galardo, Maria Noel and Riera, Maria Fernanda and Meroni, Silvina Beatriz
Journal: Molecular and cellular endocrinology (2025): 112403
Authors: Cheng, Yan and Jung, Jaekeun and Guo, Liyang and Shuboni-Mulligan, Dorela D and Chen, Jian-Fu and Hu, Wenhui and Guo, Ming-Lei
Journal: Brain, behavior, and immunity (2025): 108-122
Safety data sheet