Cell Explorer™ Live Cell Labeling Kit *Red Fluorescence*
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Excitation (nm) | 643 |
Emission (nm) | 663 |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
UNSPSC | 12352200 |
Overview | ![]() ![]() |
Excitation (nm) 643 | Emission (nm) 663 |
Platform
Fluorescence microscope
Excitation | Cy5 filter set |
Emission | Cy5 filter set |
Recommended plate | Black wall/clear bottom |
Components
Example protocol
AT A GLANCE
- Prepare cells in growth medium
- Remove the medium
Add Calcein Deep Red™ working solution (100 µL/well for 96-well plates or 25 µL/well for 384-well plates)
- Incubate cells at 37°C for 30 minutes to 2 hours
- Wash the cells
- Examine the specimen under under fluorescence microscope with Cy5 filter (Ex/Em = 646/660 nm)
Thaw all the components at room temperature before starting the experiment.
CELL PREPARATION
For guidelines on cell sample preparation, please visit https://www.aatbio.com/resources/guides/cell-sample-preparation.html
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles
Add 20 µL of DMSO into the vial of Calcein Deep Red™ (Component A) and mix well to make Calcein Deep Red™ stock solution. Note: 20 µL of Calcein Deep Red™ stock solution is enough for 1 plate. Note: Unused Calcein Deep Red™ stock solution can be aliquoted and stored at < -20 °C for 2 weeks if the tubes are sealed tightly. Avoid repeated freeze-thaw cycles and protect from light.
PREPARATION OF WORKING SOLUTION
Add 20 µL of Calcein Deep Red™ stock solution into 10 mL of HHBS (Component B) and mix well to make Calcein Deep Red™ working solution. Protect from light.
SAMPLE EXPERIMENTAL PROTOCOL
- Remove the growth medium from the cell plates. Note: It is important to remove the growth medium in order to minimize the background fluorescence and increase the signal to background ratio.
Add 100 µL/well (96-well plate) or 25 µL/well (384-well plate) Calcein Deep Red™ working solution into the cell plate.
- Incubate the cells in a 37°C, 5% CO2 incubator for 30 minutes to 2 hours.
Remove the Calcein Deep Red™ working solution from the cells.
- Wash the cells with HHBS (Component B) for 2 to 3 times, and replace with HHBS.
- Image the cells using a fluorescence microscope with Cy5 filter (Ex/Em = 646/660 nm).
Product Family
Name | Excitation (nm) | Emission (nm) |
Cell Explorer™ Live Cell Labeling Kit *Green Fluorescence* | 494 | 514 |
Images
![Image of HeLa cells stained with Cell Explorer™ Live Cell Labeling Kit *Red Fluorescence* (Cat#22609)in a Costar black wall/clear bottom 96-well plate. Cells were stained with Calcein Deep Red™ for 30 minutes and image was acquired with fluorescence microscope using Cy5 filter.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fcell-explorer-live-cell-labeling-kit-red-fluorescence%2Ffigure-for-cell-explorer-live-cell-labeling-kit-red-fluorescence_Tfi0I.jpg&w=3840&q=75)
![Flow Cytometry Analysis of Jurkat cells stained with Cell Explorer™ Live cells Labeling Kit (Cat#22609). Jurkat cells were washed once with HH buffer and stained with Calcein Deep Red™ for 30 minutes at 37C incubator. Cells were then washed with HH buffer and resuspended in HH buffer. The fluorescence intensities of Live cells (healthy, Red) and Dead cells (treated in 55°C water bath for 30 minutes, Green) were measured with NovoCyte 3000 flow cytometer using blue laser APC emission channel.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fcell-explorer-live-cell-labeling-kit-red-fluorescence%2Ffigure-for-cell-explorer-live-cell-labeling-kit-red-fluorescence_Y1p6f.jpeg&w=3840&q=75)
Citations
Authors: Cemma, Marija and Grinstein, Sergio and Brumell, John H
Journal: Autophagy (2016): 1440--1446
Authors: Hu, Qing and Li, Yuli and Miao, Guohou and Zhao, Naru and Chen, Xiaofeng
Journal: Rsc Advances (2014): 22678--22687
Authors: Myung, Ja Hye and Gajjar, Khyati A and Chen, Jihua and Molokie, Robert E and Hong, Seungpyo
Journal: Analytical chemistry (2014): 6088--6094
Authors: Lei, Bo and Chen, Xiaofeng and Han, Xue and Zhou, Jiaan
Journal: Journal of Materials Chemistry (2012): 16906--16913
References
Authors: Gough AH, Johnston PA.
Journal: Methods Mol Biol (2007): 41
Authors: Hoffman AF, Garippa RJ.
Journal: Methods Mol Biol (2007): 19
Authors: Giuliano KA., undefined
Journal: Methods Mol Biol (2007): 189
Authors: Taylor DL., undefined
Journal: Methods Mol Biol (2007): 3
Authors: Haasen D, Schnapp A, Valler MJ, Heilker R.
Journal: Methods Enzymol (2006): 121
Authors: Martinez ED, Dull AB, Beutler JA, Hager GL.
Journal: Methods Enzymol (2006): 21
Authors: Bowen WP, Wylie PG.
Journal: Assay Drug Dev Technol (2006): 209
Authors: Hudson CC, Oakley RH, Sjaastad MD, Loomis CR.
Journal: Methods Enzymol (2006): 63
Authors: Werner T, Liebisch G, Gr and l M, Schmitz G.
Journal: Cytometry A (2006): 200
Authors: Wolff M, Haasen D, Merk S, Kroner M, Maier U, Bordel S, Wiedenmann J, Nienhaus GU, Valler M, Heilker R.
Journal: Comb Chem High Throughput Screen (2006): 339
Application notes
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
Annexin V