Cell Explorer™ Fixable Live Cell Tracking Kit *Red Fluorescence*
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
UNSPSC | 12352200 |
Cell Explorer™ Fixable Live Cell Tracking Kit *Green Fluorescence* |
Overview | ![]() ![]() |
Platform
Flow cytometer
Excitation | 488 nm laser |
Emission | 610/20 nm filter |
Instrument specification(s) | PE-Texas Red channel |
Fluorescence microscope
Excitation | 570 nm |
Emission | 600 nm |
Recommended plate | Black wall/clear bottom |
Instrument specification(s) | Texas Red filter |
Components
Example protocol
AT A GLANCE
Protocol summary
- Prepare samples
- Add 100 µL/well of 1X Track It™ Red working solution
- Stain cells at 37°C for 15 minutes to 1 hour
- Wash cells
- Examine the specimen under microscope with Texas Red filter sets
Important notes
Thaw all the components to room temperature. Centrifuge component A briefly before opening.
PREPARATION OF STOCK SOLUTION
1. Track It™ Red DMSO stock solution (500X):
Add 50 µL DMSO (Component C) into the vial of Component A. Note: The unused portion of the Track It™ Red stock solution should be aliquoted as a single used vials stored at -20 oC. Avoid light and repeated freeze/thaw cycles.
PREPARATION OF WORKING SOLUTION
Dilute 500X Track It™ Red DMSO stock solution into Assay Buffer (Component B). For example, to get a 1X final concentration of Track It™ Red working solution for one 96-well microplate, dilute 20 µL of the Track It™ Red DMSO stock solution into 10 mL of Assay Buffer (Component B). Note: The final concentration of the Track It™ Red working solution should be empirically determined for different cell types and/or experimental conditions. It is recommended to test at the concentrations that are at least over a ten fold range. Note: The working solution should be prepared and used promptly. Keep from light.
For guidelines on cell sample preparation, please visit
https://www.aatbio.com/resources/guides/cell-sample-preparation.html
SAMPLE EXPERIMENTAL PROTOCOL
- Remove the cell medium, and add 100 µL/well (for 96 well plate) of 1X Track It™ Red working solution.
- Incubate the cells in a 37°C, 5% CO2 incubator for 15 minutes to 1 hour.
- Wash cells with Hanks and 20 mM Hepes buffer (HHBS) or an appropriate buffer.
- Fill the cell wells with growth medium or fix the cells (optional).
- Analyze the cells using a fluorescence microscope with Texas Red filter sets or flow cytometer with 610/20 nm filter (PE-Texas Red channel).
Images
Citations
Authors: Shiotsugu, Shohei and Okinaga, Toshinori and Habu, Manabu and Yoshiga, Daigo and Yoshioka, Izumi and Nishihara, Tatsuji and Ariyoshi, Wataru
Journal: Journal of Interferon & Cytokine Research (2019)
Authors: Cemma, Marija and Grinstein, Sergio and Brumell, John H
Journal: Autophagy (2016): 1440--1446
Authors: Myung, Ja Hye and Gajjar, Khyati A and Chen, Jihua and Molokie, Robert E and Hong, Seungpyo
Journal: Analytical chemistry (2014): 6088--6094
Authors: Lei, Bo and Chen, Xiaofeng and Han, Xue and Zhou, Jiaan
Journal: Journal of Materials Chemistry (2012): 16906--16913
References
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Journal: Methods Mol Biol (2007): 3
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Journal: Methods Enzymol (2006): 121
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Journal: Methods Enzymol (2006): 21
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Journal: Assay Drug Dev Technol (2006): 209
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Journal: Cytometry A (2006): 200
Authors: Wolff M, Haasen D, Merk S, Kroner M, Maier U, Bordel S, Wiedenmann J, Nienhaus GU, Valler M, Heilker R.
Journal: Comb Chem High Throughput Screen (2006): 339
Application notes
FAQ
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