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Caliber Fluor™ 580

Caliber Fluor™ 580 is an extremely stable fluorophore that has its absorption and fluorescence independent of pH change from pH 3 to 10. It can be used to calibrate microplate wells and cuvettes. It can also be used for testing pipetting accuracy.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Caliber Fluor™ 580 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM167.572 µL837.858 µL1.676 mL8.379 mL16.757 mL
5 mM33.514 µL167.572 µL335.143 µL1.676 mL3.351 mL
10 mM16.757 µL83.786 µL167.572 µL837.858 µL1.676 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

References

View all 8 references: Citation Explorer
A loop-mediated isothermal amplification (LAMP) assay for Strongyloides stercoralis in stool that uses a visual detection method with SYTO-82 fluorescent dye
Authors: Watts MR, James G, Sultana Y, Ginn AN, Outhred AC, Kong F, Verweij JJ, Iredell JR, Chen SC, Lee R.
Journal: Am J Trop Med Hyg (2014): 306
A new triplex real time PCR which distinguishes between MRSA, MSSA, and mecA coagulase negative strains by means of melting point analysis using SYTO 9
Authors: Weidner J, Cassens U, Gohde W, Wullenweber J, Greve B.
Journal: Clin Lab (2013): 795
Evaluation of Pseudomonas aeruginosa (PAO1) adhesion to human alveolar epithelial cells A549 using SYTO 9 dye
Authors: Larrosa M, Truchado P, Espin JC, Tomas-Barberan FA, Allende A, Garcia-Conesa MT.
Journal: Mol Cell Probes (2012): 121
Compatibility of SYTO 13 and Hoechst 33342 for longitudinal imaging of neuron viability and cell death
Authors: Hubbard KS, Gut IM, Scheeler SM, Lyman ME, McNutt PM.
Journal: BMC Res Notes (2012): 437
SYTO dyes and EvaGreen outperform SYBR Green in real-time PCR
Authors: Eischeid AC., undefined
Journal: BMC Res Notes (2011): 263
Page updated on December 17, 2024

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Catalog Number95
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Physical properties

Molecular weight

596.76

Solvent

Water

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501