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Calcein Deep Red™

Calcein AM is one the most popular fluorescent probes used for labeling and monitoring cellular functions of live cells. However, the single color of Calcein AM makes it impossible to use this valuable reagent in the multicolor applications. For example, it is impossible to use Calcein AM in GFP-tranfacted cells due to the same color to GFP. To address this color limitation of Calcein AM, we have developed Calcein Orange™, Calcein Red™ and Calcein Deep Red™. These new Calcein AM analogs enable the multicolor labeling and functional analysis of live cells in combination with Calcein AM. Calcein Deep Red™ is the esterase-hydrolyzed product of Calcein Deep Red™ acetate in cells. Calcein Deep Red™ dye can be monitored with the common Cy5 filter set. It is used as a fluorescence reference standard for Calcein Deep Red™ acetate.

Example protocol

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Calcein Deep Red™ to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM267.623 µL1.338 mL2.676 mL13.381 mL26.762 mL
5 mM53.525 µL267.623 µL535.246 µL2.676 mL5.352 mL
10 mM26.762 µL133.811 µL267.623 µL1.338 mL2.676 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Spectrum

Product family

NameExcitation (nm)Emission (nm)
Calcein Red™ AM562576
LysoBrite™ Deep Red597619

Citations

View all 19 citations: Citation Explorer
Skin-Targeted Delivery of Extracellular Vesicle-Encapsulated Curcumin Using Dissolvable Microneedle Arrays
Authors: Yerneni, Saigopalakrishna S and Yalcintas, Ezgi P and Smith, Jason D and Averick, Saadyah and Campbell, Phil G and Ozdoganlar, O Burak
Journal: Acta Biomaterialia (2022)
Radioiodination of extravesicular surface constituents to study the biocorona, cell trafficking and storage stability of extracellular vesicles
Authors: Yerneni, Saigopalakrishna S and Solomon, Talia and Smith, Jason and Campbell, Phil G
Journal: Biochimica et Biophysica Acta (BBA)-General Subjects (2022): 130069
Functional imaging of neuronal activity of auditory cortex by using Cal-520 in anesthetized and awake mice
Authors: Li, Jingcheng and Zhang, Jianxiong and Wang, Meng and Pan, Junxia and Chen, Xiaowei and Liao, Xiang
Journal: Biomedical Optics Express (2017): 2599--2610
NINJ2--A novel regulator of endothelial inflammation and activation
Authors: Wang, Jingjing and Fa, Jingjing and Wang, Pengyun and Jia, Xinzhen and Peng, Huixin and Chen, Jing and Wang, Yifan and Wang, Chenhui and Chen, Qiuyun and Tu, Xin and others, undefined
Journal: Cellular Signalling (2017)
Influence of hypothermia and subsequent rewarming upon leukocyte-endothelial interactions and expression of Junctional-Adhesion-Molecules A and B
Authors: Bogert, Nicolai V and Werner, Isabella and Kornberger, Angela and Meybohm, Patrick and Moritz, Anton and Keller, Till and Stock, Ulrich A and Beiras-Fern, undefined and ez, Andres
Journal: Scientific reports (2016)

References

View all 84 references: Citation Explorer
Functional evidence that the self-renewal gene NANOG regulates esophageal squamous cancer development
Authors: Li, Deng and Xiang, Xiaocong and Yang, Fei and Xiao, Dongqin and Liu, Kang and Chen, Zhu and Zhang, Ruolan and Feng, Gang
Journal: Biochemical and Biophysical Research Communications (2017)
Localized functional chemical stimulation of TE 671 cells cultured on nanoporous membrane by calcein and acetylcholine
Authors: Zibek S, Stett A, Koltay P, Hu M, Zengerle R, Nisch W, Stelzle M.
Journal: Biophys J. (2006)
A vaccination and challenge model using calcein marked fish
Authors: Klesius PH, Evans JJ, Shoemaker CA, Pasnik DJ.
Journal: Fish Shellfish Immunol (2006): 20
Novel fluorescence assay using calcein-AM for the determination of human erythrocyte viability and aging
Authors: Bratosin D, Mitrofan L, Palii C, Estaquier J, Montreuil J.
Journal: Cytometry A (2005): 78
Cytotoxic effects of 100 reference compounds on Hep G2 and HeLa cells and of 60 compounds on ECC-1 and CHO cells. I mechanistic assays on ROS, glutathione depletion and calcein uptake
Authors: Schoonen WG, Westerink WM, de Roos JA, Debiton E.
Journal: Toxicol In Vitro (2005): 505
Page updated on October 28, 2024

Ordering information

Price
Unit size
Catalog Number21902
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Request quotation

Physical properties

Molecular weight

373.66

Solvent

DMSO

Spectral properties

Excitation (nm)

643

Emission (nm)

663

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200

Platform

Flow cytometer

Excitation640 nm laser
Emission660, 20 nm filter
Instrument specification(s)APC channel

Fluorescence microscope

ExcitationCy5 filter set
EmissionCy5 filter set
Recommended plateBlack wall, clear bottom

Fluorescence microplate reader

Excitation620
Emission660
Cutoff630
Recommended plateSolid black
Images of Live HeLa cells stained with Calcein Deep Red&trade; (Cat#21902) and Calcein Deep Red&trade; AM(Cat.22011 ). Calcein Deep Red&trade; cannot&nbsp; permeate intact live cells.
Images of Live HeLa cells stained with Calcein Deep Red&trade; (Cat#21902) and Calcein Deep Red&trade; AM(Cat.22011 ). Calcein Deep Red&trade; cannot&nbsp; permeate intact live cells.
Images of Live HeLa cells stained with Calcein Deep Red&trade; (Cat#21902) and Calcein Deep Red&trade; AM(Cat.22011 ). Calcein Deep Red&trade; cannot&nbsp; permeate intact live cells.