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Cal-590™, sodium salt
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Rhod-2 is most commonly used among the red fluorescent calcium indicators. However, Rhod-2 AM is only moderately fluorescent in live cells upon esterase hydrolysis, and has very small cellular calcium responses. Cal-590™ has been developed to improve Rhod-2 cell loading and calcium response while maintaining the spectral wavelength of Rhod-2, making it compatible with TRITC/Cy3® filter set. In CHO and HEK cells Cal-590™ AM has cellular calcium response that is much more sensitive than Rhod-2 AM. The spectra of Cal-590 is well separated from those of FITC, Alexa Fluor® 488 and GFP, making it an ideal calcium probe for multiplexing intracellular assays with GFP cell lines or FITC/Alexa Fluor® 488 labeled antibodies.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Cal-590™, sodium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 97.43 µL | 487.149 µL | 974.298 µL | 4.871 mL | 9.743 mL |
| 5 mM | 19.486 µL | 97.43 µL | 194.86 µL | 974.298 µL | 1.949 mL |
| 10 mM | 9.743 µL | 48.715 µL | 97.43 µL | 487.149 µL | 974.298 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Quantum yield |
| Cal-590™, potassium salt | 574 | 588 | 0.621 |
| Cal-630™, sodium salt | 609 | 626 | 0.371 |
| Cal-520®, sodium salt | 492 | 515 | 0.751 |
Citations
View all 4 citations: Citation Explorer
α V β 3 Integrin regulates astrocyte reactivity
Authors: Lagos-Cabré, Raúl and Alvarez, Alvaro and Kong, Milene and Burgos-Bravo, Francesca and Cárdenas, Areli and Rojas-Mancilla, Edgardo and Pérez-Nunez, Ramón and Herrera-Molina, Rodrigo and Rojas, Fabiola and Schneider, Pascal and others, undefined
Journal: Journal of Neuroinflammation (2017): 194
Authors: Lagos-Cabré, Raúl and Alvarez, Alvaro and Kong, Milene and Burgos-Bravo, Francesca and Cárdenas, Areli and Rojas-Mancilla, Edgardo and Pérez-Nunez, Ramón and Herrera-Molina, Rodrigo and Rojas, Fabiola and Schneider, Pascal and others, undefined
Journal: Journal of Neuroinflammation (2017): 194
Ca 2+ signals initiate at immobile IP 3 receptors adjacent to ER-plasma membrane junctions
Authors: Thillaiappan, Nagendra Babu and Chavda, Alap P and Tovey, Stephen C and Prole, David L and Taylor, Colin W
Journal: Nature Communications (2017): 1505
Authors: Thillaiappan, Nagendra Babu and Chavda, Alap P and Tovey, Stephen C and Prole, David L and Taylor, Colin W
Journal: Nature Communications (2017): 1505
In vivo deep two-photon imaging of neural circuits with the fluorescent Ca2+ indicator Cal-590
Authors: Tischbirek, Carsten H and Birkner, Antje and Konnerth, Arthur
Journal: The Journal of Physiology (2016)
Authors: Tischbirek, Carsten H and Birkner, Antje and Konnerth, Arthur
Journal: The Journal of Physiology (2016)
Deep two-photon brain imaging with a red-shifted fluorometric Ca2+ indicator
Authors: Tischbirek, Carsten and Birkner, Antje and Jia, Hongbo and Sakmann, Bert and Konnerth, Arthur
Journal: Proceedings of the National Academy of Sciences (2015): 11377--11382
Authors: Tischbirek, Carsten and Birkner, Antje and Jia, Hongbo and Sakmann, Bert and Konnerth, Arthur
Journal: Proceedings of the National Academy of Sciences (2015): 11377--11382
Page updated on November 21, 2024
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