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BTC, tetrapotassium salt *CAS 216453-54-2*
This cell-impermeant coumarin Ca2+ indicator exhibits a shift in excitation maximum from about 480 nm to 400 nm upon binding Ca2+, enabling ratiometric calcium measurements. Due to its high selectivity and low affinity for Ca2+ (Kd ~7 uM) BTC is often used for the quantitation of high Ca2+levels.
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Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of BTC, tetrapotassium salt *CAS 216453-54-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 118.481 µL | 592.403 µL | 1.185 mL | 5.924 mL | 11.848 mL |
| 5 mM | 23.696 µL | 118.481 µL | 236.961 µL | 1.185 mL | 2.37 mL |
| 10 mM | 11.848 µL | 59.24 µL | 118.481 µL | 592.403 µL | 1.185 mL |
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Citations
View all 1 citations: Citation Explorer
Thallium Flux Assay for Measuring the Activity of Monovalent Cation Channels and Transporters
Authors: Weaver, C David
Journal: (2018): 105--114
Authors: Weaver, C David
Journal: (2018): 105--114
References
View all 1 references: Citation Explorer
Ionic selectivity of low-affinity ratiometric calcium indicators: mag-Fura-2, Fura-2FF and BTC
Authors: Hyrc KL, Bownik JM, Goldberg MP.
Journal: Cell Calcium (2000): 75
Authors: Hyrc KL, Bownik JM, Goldberg MP.
Journal: Cell Calcium (2000): 75
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