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Bodi Fluor™ R6G NHS Ester [equivalent to Bodipy® R6G, NHS Ester]

Bodi Fluor™R6G, NHS ester is the same molecule to Bodipy® R6G, NHS ester (Bodipy® is the trademark of Invitrogen). Bodi Fluor™R6G has the excitation and emission spectra similar to rhodamine 6G. Compared to rhodamine 6G, Bodi Fluor™R6G is a neutral molecule, and its fluorescence is not pH-dependent. Bodi Fluor™R6G gives bright green fluorescence with a sharp emission peak. Bodi Fluor™R6G has been used for developing fluorescent intracellular tracers. Its conjugates are cell-permeable as long as its carriers are cell-permeable.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Bodi Fluor™ R6G NHS Ester [equivalent to Bodipy® R6G, NHS Ester] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM228.723 µL1.144 mL2.287 mL11.436 mL22.872 mL
5 mM45.745 µL228.723 µL457.446 µL2.287 mL4.574 mL
10 mM22.872 µL114.362 µL228.723 µL1.144 mL2.287 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

Citations

View all 1 citations: Citation Explorer
A synthetic strategy for conjugation of paromomycin to cell-penetrating Tat (48-60) for delivery and visualization into Leishmania parasites
Authors: Defaus, Sira and Gallo, Maria and Abengózar, María A and Rivas, Luis and Andreu, David

References

View all 153 references: Citation Explorer
Membrane fluidity and lipid order in ternary giant unilamellar vesicles using a new bodipy-cholesterol derivative
Authors: Ariola FS, Li Z, Cornejo C, Bittman R, Heikal AA.
Journal: Biophys J (2009): 2696
Molecular imaging of lipid peroxyl radicals in living cells with a BODIPY-alpha-tocopherol adduct
Authors: Khatchadourian A, Krumova K, Boridy S, Ngo AT, Maysinger D, Cosa G.
Journal: Biochemistry (2009): 5658
Sphingosine 1-phosphate lyase enzyme assay using a BODIPY-labeled substrate
Authors: B, undefined and huvula P, Li Z, Bittman R, Saba JD.
Journal: Biochem Biophys Res Commun (2009): 366
BODIPY derivatives as donor materials for bulk heterojunction solar cells
Authors: Rousseau T, Cravino A, Bura T, Ulrich G, Ziessel R, Roncali J.
Journal: Chem Commun (Camb) (2009): 1673
Synthesis and optical properties of 2-(benzo[b]thiophene-3-yl)pyrroles and a new BODIPY fluorophore (BODIPY = 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene)
Authors: Schmidt EY, Trofimov BA, Mikhaleva AI, Zorina NV, Protzuk NI, Petrushenko KB, Ushakov IA, Dvorko MY, Meallet-Renault R, Clavier G, Vu TT, Tran HT, Pansu RB.
Journal: Chemistry (2009): 5823
Page updated on November 23, 2024

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Physical properties

Molecular weight

437.21

Solvent

DMSO

Spectral properties

Extinction coefficient (cm -1 M -1)

116000

Excitation (nm)

528

Emission (nm)

547

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501