Bodi Fluor™ R6G NHS Ester [equivalent to Bodipy® R6G, NHS Ester]

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	437.21
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	116000
Excitation (nm)	528
Emission (nm)	547

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

437.21

Extinction coefficient (cm ^-1 M ^-1)

116000

Excitation (nm)

528

Emission (nm)

547

Bodi Fluor™R6G, NHS ester is the same molecule to Bodipy® R6G, NHS ester (Bodipy® is the trademark of Invitrogen). Bodi Fluor™R6G has the excitation and emission spectra similar to rhodamine 6G. Compared to rhodamine 6G, Bodi Fluor™R6G is a neutral molecule, and its fluorescence is not pH-dependent. Bodi Fluor™R6G gives bright green fluorescence with a sharp emission peak. Bodi Fluor™R6G has been used for developing fluorescent intracellular tracers. Its conjugates are cell-permeable as long as its carriers are cell-permeable.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Bodi Fluor™ R6G NHS Ester [equivalent to Bodipy® R6G, NHS Ester] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	228.723 µL	1.144 mL	2.287 mL	11.436 mL	22.872 mL
5 mM	45.745 µL	228.723 µL	457.446 µL	2.287 mL	4.574 mL
10 mM	22.872 µL	114.362 µL	228.723 µL	1.144 mL	2.287 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	116000
Excitation (nm)	528
Emission (nm)	547

Images

Figure 1. Chemical structure for Bodi Fluor™ R6G NHS Ester [equivalent to Bodipy® R6G, NHS Ester]

Citations

View all 1 citations: Citation Explorer

A synthetic strategy for conjugation of paromomycin to cell-penetrating Tat (48-60) for delivery and visualization into Leishmania parasites
Authors: Defaus, Sira and Gallo, Maria and Abengózar, María A and Rivas, Luis and Andreu, David

References

View all 153 references: Citation Explorer

Membrane fluidity and lipid order in ternary giant unilamellar vesicles using a new bodipy-cholesterol derivative
Authors: Ariola FS, Li Z, Cornejo C, Bittman R, Heikal AA.
Journal: Biophys J (2009): 2696

Molecular imaging of lipid peroxyl radicals in living cells with a BODIPY-alpha-tocopherol adduct
Authors: Khatchadourian A, Krumova K, Boridy S, Ngo AT, Maysinger D, Cosa G.
Journal: Biochemistry (2009): 5658

Sphingosine 1-phosphate lyase enzyme assay using a BODIPY-labeled substrate
Authors: B, undefined and huvula P, Li Z, Bittman R, Saba JD.
Journal: Biochem Biophys Res Commun (2009): 366

BODIPY derivatives as donor materials for bulk heterojunction solar cells
Authors: Rousseau T, Cravino A, Bura T, Ulrich G, Ziessel R, Roncali J.
Journal: Chem Commun (Camb) (2009): 1673

Synthesis and optical properties of 2-(benzo[b]thiophene-3-yl)pyrroles and a new BODIPY fluorophore (BODIPY = 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene)
Authors: Schmidt EY, Trofimov BA, Mikhaleva AI, Zorina NV, Protzuk NI, Petrushenko KB, Ushakov IA, Dvorko MY, Meallet-Renault R, Clavier G, Vu TT, Tran HT, Pansu RB.
Journal: Chemistry (2009): 5823

Phenylethynyl-BODIPY oligomers: bright dyes and fluorescent building blocks
Authors: Cakmak Y, Akkaya EU.
Journal: Org Lett (2009): 85

Water-soluble BODIPY derivatives
Authors: Niu SL, Ulrich G, Ziessel R, Kiss A, Renard PY, Romieu A.
Journal: Org Lett (2009): 2049

Synthesis of a BODIPY library and its application to the development of live cell glucagon imaging probe
Authors: Lee JS, Kang NY, Kim YK, Samanta A, Feng S, Kim HK, Vendrell M, Park JH, Chang YT.
Journal: J Am Chem Soc (2009): 10077

Cellular localization of kinin B1 receptor in the spinal cord of streptozotocin-diabetic rats with a fluorescent [Nalpha-Bodipy]-des-Arg9-bradykinin
Authors: Talbot S, Theberge-Turmel P, Liazoghli D, Senecal J, Gaudreau P, Couture R.
Journal: J Neuroinflammation (2009): 11

Fluorescent BODIPY-labelled GM1 gangliosides designed for exploring lipid membrane properties and specific membrane-target interactions
Authors: Mikhalyov I, Gretskaya N, Johansson LB.
Journal: Chem Phys Lipids (2009): 38