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Bodi Fluor™ 488 SE [equivalent to Bodipy® FL, SE] *CAS 146616-66-2*

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Physical properties
Molecular weight389.16
SolventDMSO
Spectral properties
Correction Factor (260 nm)0.015
Correction Factor (280 nm)0.018
Extinction coefficient (cm -1 M -1)81000
Excitation (nm)504
Emission (nm)510
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

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CAS
146616-66-2
Molecular weight
389.16
Correction Factor (260 nm)
0.015
Correction Factor (280 nm)
0.018
Extinction coefficient (cm -1 M -1)
81000
Excitation (nm)
504
Emission (nm)
510
Bodi Fluor™ 488, SE is the same molecule to Bodipy® FL, SE (Bodipy® is the trademark of Invitrogen). Bodi Fluor™ 488 has the excitation and emission spectra similar to those of fluorescein. Compared to fluorescein, Bodi Fluor™ 488 is a neutral molecule, and its fluorescence is not pH-dependent. Bodi Fluor™ 488 gives bright green fluorescence with a sharp emission peak. Bodi Fluor™ 488 has been used for developing fluorescent intracellular tracers. Its conjugates are cell-permeable as long as its carriers are cell-permeable.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Bodi Fluor™ 488 SE [equivalent to Bodipy® FL, SE] *CAS 146616-66-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM256.964 µL1.285 mL2.57 mL12.848 mL25.696 mL
5 mM51.393 µL256.964 µL513.927 µL2.57 mL5.139 mL
10 mM25.696 µL128.482 µL256.964 µL1.285 mL2.57 mL

Molarity calculator

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Spectrum


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Spectral properties

Correction Factor (260 nm)0.015
Correction Factor (280 nm)0.018
Extinction coefficient (cm -1 M -1)81000
Excitation (nm)504
Emission (nm)510

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
Bodi Fluor™ 488 acid [equivalent to Bodipy® FL] *CAS 165599-63-3*504510810000.0150.018

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Citations


View all 1 citations: Citation Explorer
A synthetic strategy for conjugation of paromomycin to cell-penetrating Tat (48-60) for delivery and visualization into Leishmania parasites
Authors: Defaus, Sira and Gallo, Maria and Abeng&oacute;zar, Mar&iacute;a A and Rivas, Luis and Andreu, David

References


View all 153 references: Citation Explorer
Membrane fluidity and lipid order in ternary giant unilamellar vesicles using a new bodipy-cholesterol derivative
Authors: Ariola FS, Li Z, Cornejo C, Bittman R, Heikal AA.
Journal: Biophys J (2009): 2696
Molecular imaging of lipid peroxyl radicals in living cells with a BODIPY-alpha-tocopherol adduct
Authors: Khatchadourian A, Krumova K, Boridy S, Ngo AT, Maysinger D, Cosa G.
Journal: Biochemistry (2009): 5658
Sphingosine 1-phosphate lyase enzyme assay using a BODIPY-labeled substrate
Authors: B, undefined and huvula P, Li Z, Bittman R, Saba JD.
Journal: Biochem Biophys Res Commun (2009): 366
BODIPY derivatives as donor materials for bulk heterojunction solar cells
Authors: Rousseau T, Cravino A, Bura T, Ulrich G, Ziessel R, Roncali J.
Journal: Chem Commun (Camb) (2009): 1673
Synthesis and optical properties of 2-(benzo[b]thiophene-3-yl)pyrroles and a new BODIPY fluorophore (BODIPY = 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene)
Authors: Schmidt EY, Trofimov BA, Mikhaleva AI, Zorina NV, Protzuk NI, Petrushenko KB, Ushakov IA, Dvorko MY, Meallet-Renault R, Clavier G, Vu TT, Tran HT, Pansu RB.
Journal: Chemistry (2009): 5823
Phenylethynyl-BODIPY oligomers: bright dyes and fluorescent building blocks
Authors: Cakmak Y, Akkaya EU.
Journal: Org Lett (2009): 85
Water-soluble BODIPY derivatives
Authors: Niu SL, Ulrich G, Ziessel R, Kiss A, Renard PY, Romieu A.
Journal: Org Lett (2009): 2049
Synthesis of a BODIPY library and its application to the development of live cell glucagon imaging probe
Authors: Lee JS, Kang NY, Kim YK, Samanta A, Feng S, Kim HK, Vendrell M, Park JH, Chang YT.
Journal: J Am Chem Soc (2009): 10077
Cellular localization of kinin B1 receptor in the spinal cord of streptozotocin-diabetic rats with a fluorescent [Nalpha-Bodipy]-des-Arg9-bradykinin
Authors: Talbot S, Theberge-Turmel P, Liazoghli D, Senecal J, Gaudreau P, Couture R.
Journal: J Neuroinflammation (2009): 11
Fluorescent BODIPY-labelled GM1 gangliosides designed for exploring lipid membrane properties and specific membrane-target interactions
Authors: Mikhalyov I, Gretskaya N, Johansson LB.
Journal: Chem Phys Lipids (2009): 38