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Bodi Fluor™ 488 SE [equivalent to Bodipy® FL, SE] *CAS 146616-66-2*

Bodi Fluor™ 488, SE is the same molecule to Bodipy® FL, SE (Bodipy® is the trademark of Invitrogen). Bodi Fluor™ 488 has the excitation and emission spectra similar to those of fluorescein. Compared to fluorescein, Bodi Fluor™ 488 is a neutral molecule, and its fluorescence is not pH-dependent. Bodi Fluor™ 488 gives bright green fluorescence with a sharp emission peak. Bodi Fluor™ 488 has been used for developing fluorescent intracellular tracers. Its conjugates are cell-permeable as long as its carriers are cell-permeable.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Bodi Fluor™ 488 SE [equivalent to Bodipy® FL, SE] *CAS 146616-66-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM256.964 µL1.285 mL2.57 mL12.848 mL25.696 mL
5 mM51.393 µL256.964 µL513.927 µL2.57 mL5.139 mL
10 mM25.696 µL128.482 µL256.964 µL1.285 mL2.57 mL

Molarity calculator

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Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
Bodi Fluor™ 488 acid [equivalent to Bodipy® FL] *CAS 165599-63-3*504510810000.0150.018

Citations

View all 1 citations: Citation Explorer
A synthetic strategy for conjugation of paromomycin to cell-penetrating Tat (48-60) for delivery and visualization into Leishmania parasites
Authors: Defaus, Sira and Gallo, Maria and Abengózar, María A and Rivas, Luis and Andreu, David

References

View all 153 references: Citation Explorer
Membrane fluidity and lipid order in ternary giant unilamellar vesicles using a new bodipy-cholesterol derivative
Authors: Ariola FS, Li Z, Cornejo C, Bittman R, Heikal AA.
Journal: Biophys J (2009): 2696
Molecular imaging of lipid peroxyl radicals in living cells with a BODIPY-alpha-tocopherol adduct
Authors: Khatchadourian A, Krumova K, Boridy S, Ngo AT, Maysinger D, Cosa G.
Journal: Biochemistry (2009): 5658
Sphingosine 1-phosphate lyase enzyme assay using a BODIPY-labeled substrate
Authors: B, undefined and huvula P, Li Z, Bittman R, Saba JD.
Journal: Biochem Biophys Res Commun (2009): 366
BODIPY derivatives as donor materials for bulk heterojunction solar cells
Authors: Rousseau T, Cravino A, Bura T, Ulrich G, Ziessel R, Roncali J.
Journal: Chem Commun (Camb) (2009): 1673
Synthesis and optical properties of 2-(benzo[b]thiophene-3-yl)pyrroles and a new BODIPY fluorophore (BODIPY = 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene)
Authors: Schmidt EY, Trofimov BA, Mikhaleva AI, Zorina NV, Protzuk NI, Petrushenko KB, Ushakov IA, Dvorko MY, Meallet-Renault R, Clavier G, Vu TT, Tran HT, Pansu RB.
Journal: Chemistry (2009): 5823
Page updated on November 21, 2024

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Physical properties

Molecular weight

389.16

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.015

Correction Factor (280 nm)

0.018

Extinction coefficient (cm -1 M -1)

81000

Excitation (nm)

504

Emission (nm)

510

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

146616-66-2