Bodi Fluor FL-C12 [equivalent to BODIPY™ FL C12]

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	418.34
Solvent	DMSO

Spectral properties

Excitation (nm)	502
Emission (nm)	511

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

418.34

Excitation (nm)

502

Emission (nm)

511

Bodi Fluor FL-C12, a green fluorescent fatty acid, serves as a synthetic precursor for a wide array of fluorescent phospholipids. Unlike conventional fluorophores such as pyrene, DPH, and NBD, Bodi Fluor dyes exhibit minimal sensitivity to environmental conditions, maintaining consistent fluorescence in both aqueous and lipid mediums. The structural asymmetry of Bodi Fluor dyes allows for the derivation of a variety of fluorescent lipid analogs. Additionally, Bodi Fluor lipid droplet dyes effectively translocate across cellular membranes, specifically accumulating in polar lipids to selectively stain lipid droplets. This characteristic renders them applicable for the staining of both live and fixed cells in cellular biology research.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Bodi Fluor FL-C12 [equivalent to BODIPY™ FL C12] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	239.04 µL	1.195 mL	2.39 mL	11.952 mL	23.904 mL
5 mM	47.808 µL	239.04 µL	478.08 µL	2.39 mL	4.781 mL
10 mM	23.904 µL	119.52 µL	239.04 µL	1.195 mL	2.39 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Excitation (nm)	502
Emission (nm)	511

Images

Figure 1. BODIPY™ FL C12

References

View all 50 references: Citation Explorer

Identification of Leishmania donovani PEX5-PTS1 Interaction Inhibitors through Fluorescence Polarization-Based High-Throughput Screening.
Authors: Phan, Trong-Nhat and Park, Kyu-Ho Paul and Shum, David and No, Joo Hwan
Journal: Molecules (Basel, Switzerland) (2024)

Contact-FP: A Dimerization-Dependent Fluorescent Protein Toolkit for Visualizing Membrane Contact Site Dynamics.
Authors: Miner, Gregory E and Smith, Sidney Y and Showalter, Wendy K and So, Christina M and Ragusa, Joey V and Powers, Alex E and Zanellati, Maria Clara and Hsu, Chih-Hsuan and Marchan, Michelle F and Cohen, Sarah
Journal: Contact (Thousand Oaks (Ventura County, Calif.)) (2024): 25152564241228911

Fluorescent fatty acid conjugates for live cell imaging of peroxisomes.
Authors: Korotkova, Daria and Borisyuk, Anya and Guihur, Anthony and Bardyn, Manon and Kuttler, Fabien and Reymond, Luc and Schuhmacher, Milena and Amen, Triana
Journal: Nature communications (2024): 4314

Lysosome targeting fluorescent probe for NAAA imaging and its applications in the drug development for anti-inflammatory.
Authors: Zhou, Limin and Tian, Manman and Zhang, Baojing and Cao, Xudong and Huo, Xiaokui and Yang, Fangyu and Cao, Peng and Feng, Lei and Ma, Xiaochi and Tian, Xiangge
Journal: International journal of biological macromolecules (2024): 130307

Illuminating the Cryptococcus neoformans Species Complex: Unveiling Intracellular Structures with Fluorescent-Protein-Based Markers.
Authors: Shi, Ran and Lin, Xiaorong
Journal: Genetics (2024)

Assay of Reactive Oxygen/Nitrogen Species (ROS/RNS) in Arabidopsis Peroxisomes Through Fluorescent Protein Containing a Type 1 Peroxisomal Targeting Signal (PTS1).
Authors: Corpas, Francisco J and Palma, José M
Journal: Methods in molecular biology (Clifton, N.J.) (2023): 149-160

Exploration of chlorophyll fluorescence characteristics gene regulatory in rice (Oryza sativa L.): a genome-wide association study.
Authors: Liu, Sicheng and Xiong, Zhuang and Zhang, Zuolin and Wei, Youbo and Xiong, Dongliang and Wang, Fei and Huang, Jianliang
Journal: Frontiers in plant science (2023): 1234866

Assessment of the Peroxisomal Redox State in Living Cells Using NADPH- and NAD+/NADH-Specific Fluorescent Protein Sensors.
Authors: Costa, Cláudio F and Li, Hongli and Hussein, Mohamed A F and Yang, Yi and Lismont, Celien and Fransen, Marc
Journal: Methods in molecular biology (Clifton, N.J.) (2023): 183-197

Fluorescent Fusion Protein Expression in Plant Cells.
Authors: Huang, Shuxian and Yonglun, Zeng
Journal: Methods in molecular biology (Clifton, N.J.) (2023): 119-127

A Medicago truncatula Cell Biology Resource: Transgenic Lines Expressing Fluorescent Protein-Based Markers of Membranes, Organelles, and Subcellular Compartments.
Authors: Ivanov, Sergey and Daniels, Dierdra A and Harrison, Maria J
Journal: Molecular plant-microbe interactions : MPMI (2023): 256-259