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Bodi Fluor™ 488 acid [equivalent to Bodipy® FL] *CAS 165599-63-3*
Bodi Fluor™ 488 is the same molecule to Bodipy® FL (Bodipy® is the trademark of Invitrogen). Bodi Fluor™ 488 has the excitation and emission spectra similar to those of fluorescein. Compared to fluorescein, Bodi Fluor™ 488 is a neutral molecule, and its fluorescence is not pH-dependent. Bodi Fluor™ 488 gives bright green fluorescence with a sharp emission peak. Bodi Fluor™ 488 has been used for developing fluorescent intracellular tracers. Its conjugates are cell-permeable as long as its carriers are cell-permeable.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Bodi Fluor™ 488 acid [equivalent to Bodipy® FL] *CAS 165599-63-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 342.36 µL | 1.712 mL | 3.424 mL | 17.118 mL | 34.236 mL |
| 5 mM | 68.472 µL | 342.36 µL | 684.72 µL | 3.424 mL | 6.847 mL |
| 10 mM | 34.236 µL | 171.18 µL | 342.36 µL | 1.712 mL | 3.424 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
| Bodi Fluor™ 488 SE [equivalent to Bodipy® FL, SE] *CAS 146616-66-2* | 504 | 510 | 81000 | 0.015 | 0.018 |
Citations
View all 2 citations: Citation Explorer
A synthetic strategy for conjugation of paromomycin to cell-penetrating TAT (48-60) for delivery and visualization into Leishmania parasites
Authors: Defaus, Sira and Gallo, Maria and Abeng{\'o}zar, Mar{\'\i}a A and Rivas, Luis and Andreu Mart{\'\i}nez, David
Journal: International Journal of Peptides. 2017; 2017: 4213037 (2017)
Authors: Defaus, Sira and Gallo, Maria and Abeng{\'o}zar, Mar{\'\i}a A and Rivas, Luis and Andreu Mart{\'\i}nez, David
Journal: International Journal of Peptides. 2017; 2017: 4213037 (2017)
A synthetic strategy for conjugation of paromomycin to cell-penetrating Tat (48-60) for delivery and visualization into Leishmania parasites
Authors: Defaus, Sira and Gallo, Maria and Abengózar, María A and Rivas, Luis and Andreu, David
Authors: Defaus, Sira and Gallo, Maria and Abengózar, María A and Rivas, Luis and Andreu, David
References
View all 153 references: Citation Explorer
Fluorescent BODIPY-labelled GM1 gangliosides designed for exploring lipid membrane properties and specific membrane-target interactions
Authors: Mikhalyov I, Gretskaya N, Johansson LB.
Journal: Chem Phys Lipids (2009): 38
Authors: Mikhalyov I, Gretskaya N, Johansson LB.
Journal: Chem Phys Lipids (2009): 38
Synthesis of a BODIPY library and its application to the development of live cell glucagon imaging probe
Authors: Lee JS, Kang NY, Kim YK, Samanta A, Feng S, Kim HK, Vendrell M, Park JH, Chang YT.
Journal: J Am Chem Soc (2009): 10077
Authors: Lee JS, Kang NY, Kim YK, Samanta A, Feng S, Kim HK, Vendrell M, Park JH, Chang YT.
Journal: J Am Chem Soc (2009): 10077
Molecular imaging of lipid peroxyl radicals in living cells with a BODIPY-alpha-tocopherol adduct
Authors: Khatchadourian A, Krumova K, Boridy S, Ngo AT, Maysinger D, Cosa G.
Journal: Biochemistry (2009): 5658
Authors: Khatchadourian A, Krumova K, Boridy S, Ngo AT, Maysinger D, Cosa G.
Journal: Biochemistry (2009): 5658
Sphingosine 1-phosphate lyase enzyme assay using a BODIPY-labeled substrate
Authors: B, undefined and huvula P, Li Z, Bittman R, Saba JD.
Journal: Biochem Biophys Res Commun (2009): 366
Authors: B, undefined and huvula P, Li Z, Bittman R, Saba JD.
Journal: Biochem Biophys Res Commun (2009): 366
BODIPY derivatives as donor materials for bulk heterojunction solar cells
Authors: Rousseau T, Cravino A, Bura T, Ulrich G, Ziessel R, Roncali J.
Journal: Chem Commun (Camb) (2009): 1673
Authors: Rousseau T, Cravino A, Bura T, Ulrich G, Ziessel R, Roncali J.
Journal: Chem Commun (Camb) (2009): 1673
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