Bodi Fluor™ 488 acid [equivalent to Bodipy® FL] *CAS 165599-63-3*
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Physical properties
Molecular weight | 292.09 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.015 |
Correction Factor (280 nm) | 0.018 |
Extinction coefficient (cm -1 M -1) | 81000 |
Excitation (nm) | 504 |
Emission (nm) | 510 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
Overview | ![]() ![]() |
CAS 165599-63-3 | Molecular weight 292.09 | Correction Factor (260 nm) 0.015 | Correction Factor (280 nm) 0.018 | Extinction coefficient (cm -1 M -1) 81000 | Excitation (nm) 504 | Emission (nm) 510 |
Bodi Fluor™ 488 is the same molecule to Bodipy® FL (Bodipy® is the trademark of Invitrogen). Bodi Fluor™ 488 has the excitation and emission spectra similar to those of fluorescein. Compared to fluorescein, Bodi Fluor™ 488 is a neutral molecule, and its fluorescence is not pH-dependent. Bodi Fluor™ 488 gives bright green fluorescence with a sharp emission peak. Bodi Fluor™ 488 has been used for developing fluorescent intracellular tracers. Its conjugates are cell-permeable as long as its carriers are cell-permeable.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Bodi Fluor™ 488 acid [equivalent to Bodipy® FL] *CAS 165599-63-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 342.36 µL | 1.712 mL | 3.424 mL | 17.118 mL | 34.236 mL |
5 mM | 68.472 µL | 342.36 µL | 684.72 µL | 3.424 mL | 6.847 mL |
10 mM | 34.236 µL | 171.18 µL | 342.36 µL | 1.712 mL | 3.424 mL |
Molarity calculator
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Spectrum
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![spectrum](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fspectra%2Fbodi_fluor_488.png&w=2048&q=50)
![spectrum](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fspectra%2Fbodi_fluor_488.png&w=2048&q=50)
Spectral properties
Correction Factor (260 nm) | 0.015 |
Correction Factor (280 nm) | 0.018 |
Extinction coefficient (cm -1 M -1) | 81000 |
Excitation (nm) | 504 |
Emission (nm) | 510 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
Bodi Fluor™ 488 SE [equivalent to Bodipy® FL, SE] *CAS 146616-66-2* | 504 | 510 | 81000 | 0.015 | 0.018 |
Images
Citations
View all 2 citations: Citation Explorer
A synthetic strategy for conjugation of paromomycin to cell-penetrating TAT (48-60) for delivery and visualization into Leishmania parasites
Authors: Defaus, Sira and Gallo, Maria and Abeng{\'o}zar, Mar{\'\i}a A and Rivas, Luis and Andreu Mart{\'\i}nez, David
Journal: International Journal of Peptides. 2017; 2017: 4213037 (2017)
Authors: Defaus, Sira and Gallo, Maria and Abeng{\'o}zar, Mar{\'\i}a A and Rivas, Luis and Andreu Mart{\'\i}nez, David
Journal: International Journal of Peptides. 2017; 2017: 4213037 (2017)
A synthetic strategy for conjugation of paromomycin to cell-penetrating Tat (48-60) for delivery and visualization into Leishmania parasites
Authors: Defaus, Sira and Gallo, Maria and Abengózar, María A and Rivas, Luis and Andreu, David
Authors: Defaus, Sira and Gallo, Maria and Abengózar, María A and Rivas, Luis and Andreu, David
References
View all 153 references: Citation Explorer
Fluorescent BODIPY-labelled GM1 gangliosides designed for exploring lipid membrane properties and specific membrane-target interactions
Authors: Mikhalyov I, Gretskaya N, Johansson LB.
Journal: Chem Phys Lipids (2009): 38
Authors: Mikhalyov I, Gretskaya N, Johansson LB.
Journal: Chem Phys Lipids (2009): 38
Synthesis of a BODIPY library and its application to the development of live cell glucagon imaging probe
Authors: Lee JS, Kang NY, Kim YK, Samanta A, Feng S, Kim HK, Vendrell M, Park JH, Chang YT.
Journal: J Am Chem Soc (2009): 10077
Authors: Lee JS, Kang NY, Kim YK, Samanta A, Feng S, Kim HK, Vendrell M, Park JH, Chang YT.
Journal: J Am Chem Soc (2009): 10077
Molecular imaging of lipid peroxyl radicals in living cells with a BODIPY-alpha-tocopherol adduct
Authors: Khatchadourian A, Krumova K, Boridy S, Ngo AT, Maysinger D, Cosa G.
Journal: Biochemistry (2009): 5658
Authors: Khatchadourian A, Krumova K, Boridy S, Ngo AT, Maysinger D, Cosa G.
Journal: Biochemistry (2009): 5658
Sphingosine 1-phosphate lyase enzyme assay using a BODIPY-labeled substrate
Authors: B, undefined and huvula P, Li Z, Bittman R, Saba JD.
Journal: Biochem Biophys Res Commun (2009): 366
Authors: B, undefined and huvula P, Li Z, Bittman R, Saba JD.
Journal: Biochem Biophys Res Commun (2009): 366
BODIPY derivatives as donor materials for bulk heterojunction solar cells
Authors: Rousseau T, Cravino A, Bura T, Ulrich G, Ziessel R, Roncali J.
Journal: Chem Commun (Camb) (2009): 1673
Authors: Rousseau T, Cravino A, Bura T, Ulrich G, Ziessel R, Roncali J.
Journal: Chem Commun (Camb) (2009): 1673
Synthesis and optical properties of 2-(benzo[b]thiophene-3-yl)pyrroles and a new BODIPY fluorophore (BODIPY = 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene)
Authors: Schmidt EY, Trofimov BA, Mikhaleva AI, Zorina NV, Protzuk NI, Petrushenko KB, Ushakov IA, Dvorko MY, Meallet-Renault R, Clavier G, Vu TT, Tran HT, Pansu RB.
Journal: Chemistry (2009): 5823
Authors: Schmidt EY, Trofimov BA, Mikhaleva AI, Zorina NV, Protzuk NI, Petrushenko KB, Ushakov IA, Dvorko MY, Meallet-Renault R, Clavier G, Vu TT, Tran HT, Pansu RB.
Journal: Chemistry (2009): 5823
Phenylethynyl-BODIPY oligomers: bright dyes and fluorescent building blocks
Authors: Cakmak Y, Akkaya EU.
Journal: Org Lett (2009): 85
Authors: Cakmak Y, Akkaya EU.
Journal: Org Lett (2009): 85
Water-soluble BODIPY derivatives
Authors: Niu SL, Ulrich G, Ziessel R, Kiss A, Renard PY, Romieu A.
Journal: Org Lett (2009): 2049
Authors: Niu SL, Ulrich G, Ziessel R, Kiss A, Renard PY, Romieu A.
Journal: Org Lett (2009): 2049
Cellular localization of kinin B1 receptor in the spinal cord of streptozotocin-diabetic rats with a fluorescent [Nalpha-Bodipy]-des-Arg9-bradykinin
Authors: Talbot S, Theberge-Turmel P, Liazoghli D, Senecal J, Gaudreau P, Couture R.
Journal: J Neuroinflammation (2009): 11
Authors: Talbot S, Theberge-Turmel P, Liazoghli D, Senecal J, Gaudreau P, Couture R.
Journal: J Neuroinflammation (2009): 11
Membrane fluidity and lipid order in ternary giant unilamellar vesicles using a new bodipy-cholesterol derivative
Authors: Ariola FS, Li Z, Cornejo C, Bittman R, Heikal AA.
Journal: Biophys J (2009): 2696
Authors: Ariola FS, Li Z, Cornejo C, Bittman R, Heikal AA.
Journal: Biophys J (2009): 2696