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Bodi Fluor™ 488 acid [equivalent to Bodipy® FL] *CAS 165599-63-3*

Bodi Fluor™ 488 is the same molecule to Bodipy® FL (Bodipy® is the trademark of Invitrogen). Bodi Fluor™ 488 has the excitation and emission spectra similar to those of fluorescein. Compared to fluorescein, Bodi Fluor™ 488 is a neutral molecule, and its fluorescence is not pH-dependent. Bodi Fluor™ 488 gives bright green fluorescence with a sharp emission peak. Bodi Fluor™ 488 has been used for developing fluorescent intracellular tracers. Its conjugates are cell-permeable as long as its carriers are cell-permeable.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Bodi Fluor™ 488 acid [equivalent to Bodipy® FL] *CAS 165599-63-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM342.36 µL1.712 mL3.424 mL17.118 mL34.236 mL
5 mM68.472 µL342.36 µL684.72 µL3.424 mL6.847 mL
10 mM34.236 µL171.18 µL342.36 µL1.712 mL3.424 mL

Molarity calculator

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Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
Bodi Fluor™ 488 SE [equivalent to Bodipy® FL, SE] *CAS 146616-66-2*504510810000.0150.018

Citations

View all 2 citations: Citation Explorer
A synthetic strategy for conjugation of paromomycin to cell-penetrating TAT (48-60) for delivery and visualization into Leishmania parasites
Authors: Defaus, Sira and Gallo, Maria and Abeng{\'o}zar, Mar{\'\i}a A and Rivas, Luis and Andreu Mart{\'\i}nez, David
Journal: International Journal of Peptides. 2017; 2017: 4213037 (2017)
A synthetic strategy for conjugation of paromomycin to cell-penetrating Tat (48-60) for delivery and visualization into Leishmania parasites
Authors: Defaus, Sira and Gallo, Maria and Abengózar, María A and Rivas, Luis and Andreu, David

References

View all 153 references: Citation Explorer
Fluorescent BODIPY-labelled GM1 gangliosides designed for exploring lipid membrane properties and specific membrane-target interactions
Authors: Mikhalyov I, Gretskaya N, Johansson LB.
Journal: Chem Phys Lipids (2009): 38
Synthesis of a BODIPY library and its application to the development of live cell glucagon imaging probe
Authors: Lee JS, Kang NY, Kim YK, Samanta A, Feng S, Kim HK, Vendrell M, Park JH, Chang YT.
Journal: J Am Chem Soc (2009): 10077
Molecular imaging of lipid peroxyl radicals in living cells with a BODIPY-alpha-tocopherol adduct
Authors: Khatchadourian A, Krumova K, Boridy S, Ngo AT, Maysinger D, Cosa G.
Journal: Biochemistry (2009): 5658
Sphingosine 1-phosphate lyase enzyme assay using a BODIPY-labeled substrate
Authors: B, undefined and huvula P, Li Z, Bittman R, Saba JD.
Journal: Biochem Biophys Res Commun (2009): 366
BODIPY derivatives as donor materials for bulk heterojunction solar cells
Authors: Rousseau T, Cravino A, Bura T, Ulrich G, Ziessel R, Roncali J.
Journal: Chem Commun (Camb) (2009): 1673
Page updated on November 21, 2024

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Physical properties

Molecular weight

292.09

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.015

Correction Factor (280 nm)

0.018

Extinction coefficient (cm -1 M -1)

81000

Excitation (nm)

504

Emission (nm)

510

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

165599-63-3