Calculator
BOC-Val-Pro-Arg-AMC
BOC-Val-Pro-Arg-AMC is a sensitive fluorogenic substrate for alpha-thrombin. The non-fluorescent substrate generates a bright blue fluorescent AMC product that has an emission spectra that can be easily detected with the DAPI filter set. The Km values of alpha-thrombin and the alpha-thrombin-staphylocoagulase complex for BOC-Val-Pro-Arg-AMC were reported to be 21 and 25 uM respectively. The Kcat values of alpha-thrombin and the alpha-thrombin-staphylocoagulase complex for BOC-Val-Pro-Arg-AMC were reported to be 105 and 89 s-1 respectively. This substrate has also been used for the rapid detection of MRSA (methicillin-resistant Staphylococcus aureus).
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of BOC-Val-Pro-Arg-AMC to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 134.816 µL | 674.082 µL | 1.348 mL | 6.741 mL | 13.482 mL |
| 5 mM | 26.963 µL | 134.816 µL | 269.633 µL | 1.348 mL | 2.696 mL |
| 10 mM | 13.482 µL | 67.408 µL | 134.816 µL | 674.082 µL | 1.348 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| BOC-Val-Pro-Arg-Aminoluciferin | 362 | 499 | 18000 |
Citations
View all 1 citations: Citation Explorer
Using tungsten oxide quantum-dot enhanced electrochemiluminescence to measure thrombin activity and screen its inhibitors
Authors: Zhang, Jing and Xu, Dan and Deng, Zijie and Tan, Xueping and Guo, Dongnan and Qiao, Yanru and Li, You and Hou, Xiaofang and Wang, Sicen and Zhang, Junbo
Journal: Talanta (2023): 125267
Authors: Zhang, Jing and Xu, Dan and Deng, Zijie and Tan, Xueping and Guo, Dongnan and Qiao, Yanru and Li, You and Hou, Xiaofang and Wang, Sicen and Zhang, Junbo
Journal: Talanta (2023): 125267
References
View all 120 references: Citation Explorer
Improved detection of ovarian cancer metastases by intraoperative quantitative fluorescence protease imaging in a pre-clinical model
Authors: Sheth RA, Upadhyay R, Stangenberg L, Sheth R, Weissleder R, Mahmood U.
Journal: Gynecol Oncol (2009): 616
Authors: Sheth RA, Upadhyay R, Stangenberg L, Sheth R, Weissleder R, Mahmood U.
Journal: Gynecol Oncol (2009): 616
A fluorescence lifetime-based assay for protease inhibitor profiling on human kallikrein 7
Authors: Doering K, Meder G, Hinnenberger M, Woelcke J, Mayr LM, Hassiepen U.
Journal: J Biomol Screen (2009): 1
Authors: Doering K, Meder G, Hinnenberger M, Woelcke J, Mayr LM, Hassiepen U.
Journal: J Biomol Screen (2009): 1
A protease inhibitor discovery method using fluorescence correlation spectroscopy with position-specific labeled protein substrates
Authors: Nakata H, Ohtsuki T, Sisido M.
Journal: Anal Biochem (2009): 121
Authors: Nakata H, Ohtsuki T, Sisido M.
Journal: Anal Biochem (2009): 121
Label-free, homogeneous, and fluorescence "turn-on" detection of protease using conjugated polyelectrolytes
Authors: An L, Liu L, Wang S.
Journal: Biomacromolecules (2009): 454
Authors: An L, Liu L, Wang S.
Journal: Biomacromolecules (2009): 454
Fluorescence resonance energy transfer-based assay for characterization of hepatitis C virus NS3-4A protease activity in live cells
Authors: Sabariegos R, Picazo F, Domingo B, Franco S, Martinez MA, Llopis J.
Journal: Antimicrob Agents Chemother (2009): 728
Authors: Sabariegos R, Picazo F, Domingo B, Franco S, Martinez MA, Llopis J.
Journal: Antimicrob Agents Chemother (2009): 728
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