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Benzoyl-Leu-Gly-Arg-Aminoluciferin
This aminoluciferin-based probe is a luminogenic substrate for complement component C3/C5 convertases, coagulation factor Xa, and soybean trypsin-like enzyme. It is also hydrolyzed by macropain, a high molecular weight thiol proteinase from human erythrocytes. The protease-released D-aminoluciferin can be detected using our luciferase assay kits. Benzoyl-Leu-Gly-Arg-Aminoluciferin is much more sensitive than Boc-Leu-Gly-Arg-AMC, the widely used fluorogenic AMC substrate.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Benzoyl-Leu-Gly-Arg-Aminoluciferin to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 121.38 µL | 606.899 µL | 1.214 mL | 6.069 mL | 12.138 mL |
| 5 mM | 24.276 µL | 121.38 µL | 242.76 µL | 1.214 mL | 2.428 mL |
| 10 mM | 12.138 µL | 60.69 µL | 121.38 µL | 606.899 µL | 1.214 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| BOC-Leu-Gly-Arg-Aminoluciferin | 362 | 499 | 18000 |
Citations
View all 2 citations: Citation Explorer
Improved bioluminescence-based endotoxin measurement method using a salt-resistant luciferase mutant
Authors: Yawata, Satoshi and Noda, Kenichi and Shimomura, Ai and Kuroda, Akio
Journal: Analytical Biochemistry (2021): 114408
Authors: Yawata, Satoshi and Noda, Kenichi and Shimomura, Ai and Kuroda, Akio
Journal: Analytical Biochemistry (2021): 114408
Endotoxin assay by bioluminescence using mutant firefly luciferase
Authors: Noda, Kenichi and Goto, Hitoshi and Murakami, Yuji and Ahmed, Abo Bakr F and Kuroda, Akio
Journal: Analytical biochemistry (2010): 152--155
Authors: Noda, Kenichi and Goto, Hitoshi and Murakami, Yuji and Ahmed, Abo Bakr F and Kuroda, Akio
Journal: Analytical biochemistry (2010): 152--155
References
View all 16 references: Citation Explorer
Identification of mutant firefly luciferases that efficiently utilize aminoluciferins
Authors: Harwood KR, Mofford DM, Reddy GR, Miller SC.
Journal: Chem Biol (2011): 1649
Authors: Harwood KR, Mofford DM, Reddy GR, Miller SC.
Journal: Chem Biol (2011): 1649
In vivo imaging of early stage apoptosis by measuring real-time caspase-3/7 activation
Authors: Scabini M, Stellari F, Cappella P, Rizzitano S, Texido G, Pesenti E.
Journal: Apoptosis (2011): 198
Authors: Scabini M, Stellari F, Cappella P, Rizzitano S, Texido G, Pesenti E.
Journal: Apoptosis (2011): 198
Aminoluciferins as functional bioluminogenic substrates of firefly luciferase
Authors: Takakura H, Kojima R, Urano Y, Terai T, Hanaoka K, Nagano T.
Journal: Chem Asian J (2011): 1800
Authors: Takakura H, Kojima R, Urano Y, Terai T, Hanaoka K, Nagano T.
Journal: Chem Asian J (2011): 1800
Noninvasive molecular imaging of apoptosis in vivo using a modified firefly luciferase substrate, Z-DEVD-aminoluciferin
Authors: Hickson J, Ackler S, Klaubert D, Bouska J, Ellis P, Foster K, Oleksijew A, Rodriguez L, Schlessinger S, Wang B, Frost D.
Journal: Cell Death Differ (2010): 1003
Authors: Hickson J, Ackler S, Klaubert D, Bouska J, Ellis P, Foster K, Oleksijew A, Rodriguez L, Schlessinger S, Wang B, Frost D.
Journal: Cell Death Differ (2010): 1003
Robust light emission from cyclic alkylaminoluciferin substrates for firefly luciferase
Authors: Reddy, G. R.; Thompson, W. C.; Miller, S. C.
Journal: J Am Chem Soc (2010): 13586-7
Authors: Reddy, G. R.; Thompson, W. C.; Miller, S. C.
Journal: J Am Chem Soc (2010): 13586-7
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