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bBBr [Dibromobimane] *CAS 68654-25-1*
dBBr is one of the most popular thiol-reactive fluorescent tags, and is widely used to detect various thiol-containing biomolecules such as glutathione in cells. It is fluorogenic upon reacting with thiol-containing molecules. Dibromobimane is a unique fluorogenic crosslinking reagent for proteins because it is unlikely to fluoresce until both of its alkylating groups have reacted. It has been used to crosslink thiols in myosin, hemoglobin, Escherichia coli lactose permease and mitochondrial ATPase. Dibromobimane is also used to probe for the proximity of dual-cysteine mutagenesis sites in ArsA ATPase and to crosslink thiols in actin, myosin and P-glycoprotein.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of bBBr [Dibromobimane] *CAS 68654-25-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 285.706 µL | 1.429 mL | 2.857 mL | 14.285 mL | 28.571 mL |
| 5 mM | 57.141 µL | 285.706 µL | 571.412 µL | 2.857 mL | 5.714 mL |
| 10 mM | 28.571 µL | 142.853 µL | 285.706 µL | 1.429 mL | 2.857 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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References
View all 25 references: Citation Explorer
Reactivity of zinc finger cysteines: chemical modifications within labile zinc fingers in estrogen receptor
Authors: Atsriku C, Scott GK, Benz CC, Baldwin MA.
Journal: J Am Soc Mass Spectrom (2005): 2017
Authors: Atsriku C, Scott GK, Benz CC, Baldwin MA.
Journal: J Am Soc Mass Spectrom (2005): 2017
Interaction of S100A8/S100A9-arachidonic acid complexes with the scavenger receptor CD36 may facilitate fatty acid uptake by endothelial cells
Authors: Kerkhoff C, Sorg C, T and on NN, Nacken W.
Journal: Biochemistry (2001): 241
Authors: Kerkhoff C, Sorg C, T and on NN, Nacken W.
Journal: Biochemistry (2001): 241
Determination of plasma homocysteine by high performance liquid chromatography with fluorescence detection
Authors: Liao Y, Liang YQ, Zhi XM, Liao ZH.
Journal: Se Pu (2000): 49
Authors: Liao Y, Liang YQ, Zhi XM, Liao ZH.
Journal: Se Pu (2000): 49
Plasma cysteine deficiency and decreased reduction of nitrososulfamethoxazole with HIV infection
Authors: Naisbitt DJ, Vilar FJ, Stalford AC, Wilkins EG, Pirmohamed M, Park BK.
Journal: AIDS Res Hum Retroviruses (2000): 1929
Authors: Naisbitt DJ, Vilar FJ, Stalford AC, Wilkins EG, Pirmohamed M, Park BK.
Journal: AIDS Res Hum Retroviruses (2000): 1929
Fatty acid translocase/CD36 mediates the uptake of palmitate by type II pneumocytes
Authors: Guthmann F, Haupt R, Looman AC, Spener F, Rustow B.
Journal: Am J Physiol (1999): L191
Authors: Guthmann F, Haupt R, Looman AC, Spener F, Rustow B.
Journal: Am J Physiol (1999): L191
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