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BAPTA, AM *CAS 126150-97-8*
BAPTA AM is cell-permeable version of BAPTA (1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid), a cell-permeable calcium chelator. This BAPTA derivative is used for adjusting calcium concentrations in cells and tissues. BAPTA is a calcium-specific aminopolycarboxylic acid. The presence of four carboxylic acid functional groups makes possible the binding of two calcium ions. The extensive flexibility of the carboxylate ligands is critical to the coordination of calcium and other metal ions.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of BAPTA, AM *CAS 126150-97-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 130.774 µL | 653.868 µL | 1.308 mL | 6.539 mL | 13.077 mL |
| 5 mM | 26.155 µL | 130.774 µL | 261.547 µL | 1.308 mL | 2.615 mL |
| 10 mM | 13.077 µL | 65.387 µL | 130.774 µL | 653.868 µL | 1.308 mL |
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Citations
View all 11 citations: Citation Explorer
Regulation of Calcium entry by cyclic GMP signaling in Toxoplasma gondii
Authors: Triana, Miryam A Hortua and M{\'a}rquez-Nogueras, Karla M and Fazli, Mojtaba Sedigh and Quinn, Shannon and Moreno, Silvia NJ
Journal: Journal of Biological Chemistry (2024): 105771
Authors: Triana, Miryam A Hortua and M{\'a}rquez-Nogueras, Karla M and Fazli, Mojtaba Sedigh and Quinn, Shannon and Moreno, Silvia NJ
Journal: Journal of Biological Chemistry (2024): 105771
RNA granules hitchhike on lysosomes for long-distance transport, using annexin A11 as a molecular tether
Authors: Liao, Ya-Cheng and Fernandopulle, Michael S and Wang, Guozhen and Choi, Heejun and Hao, Ling and Drerup, Catherine M and Patel, Rajan and Qamar, Seema and Nixon-Abell, Jonathon and Shen, Yi and others,
Journal: Cell (2019): 147--164
Authors: Liao, Ya-Cheng and Fernandopulle, Michael S and Wang, Guozhen and Choi, Heejun and Hao, Ling and Drerup, Catherine M and Patel, Rajan and Qamar, Seema and Nixon-Abell, Jonathon and Shen, Yi and others,
Journal: Cell (2019): 147--164
The role of calcium signalling in autophagy
Authors: Chehab, Tala
Journal: (2018)
Authors: Chehab, Tala
Journal: (2018)
Angiotensin II regulates phosphorylation of actin-associated proteins in human podocytes
Authors: Schenk, Laura K and M{\"o}ller-Kerutt, Annika and Klosowski, Rafael and Wolters, Dirk and Schaffner-Reckinger, Elisabeth and Weide, Thomas and Pavenst{\"a}dt, Hermann and Vollenbr{\"o}ker, Beate
Journal: The FASEB Journal (2017): 5019--5035
Authors: Schenk, Laura K and M{\"o}ller-Kerutt, Annika and Klosowski, Rafael and Wolters, Dirk and Schaffner-Reckinger, Elisabeth and Weide, Thomas and Pavenst{\"a}dt, Hermann and Vollenbr{\"o}ker, Beate
Journal: The FASEB Journal (2017): 5019--5035
Licochalcone A induces T24 bladder cancer cell apoptosis by increasing intracellular calcium levels
Authors: Yang, Xinhui and Jiang, Jiangtao and Yang, Xinyan and Han, Jichun and Zheng, Qiusheng
Journal: Molecular medicine reports (2016): 911--919
Authors: Yang, Xinhui and Jiang, Jiangtao and Yang, Xinyan and Han, Jichun and Zheng, Qiusheng
Journal: Molecular medicine reports (2016): 911--919
References
View all 43 references: Citation Explorer
Determination of BAPTA-AM, the acetoxymethyl tetraester of BAPTA, in rat plasma by liquid chromatography tandem mass spectrometry
Authors: Zheng F, Hang T, Wu C, Di B, Liu W, Xu J, Zhai C, Sun D, Liu J, Wei W.
Journal: J Mass Spectrom (2006): 1615
Authors: Zheng F, Hang T, Wu C, Di B, Liu W, Xu J, Zhai C, Sun D, Liu J, Wei W.
Journal: J Mass Spectrom (2006): 1615
Abrogation of DTH response and mitogenic lectin- and alloantigen-induced activation of lymphocytes by calcium inhibitors TMB-8 and BAPTA-AM
Authors: Saxena HM, Dikshit M.
Journal: Immunol Lett (2005): 60
Authors: Saxena HM, Dikshit M.
Journal: Immunol Lett (2005): 60
BAPTA induces frequency shifts in vivo of spontaneous otoacoustic emissions of the bobtail lizard
Authors: Manley GA, Kirk DL.
Journal: Audiol Neurootol (2005): 248
Authors: Manley GA, Kirk DL.
Journal: Audiol Neurootol (2005): 248
Ca2+-dependent interaction of BAPTA with phospholipids
Authors: Rousset M, Cens T, Vanmau N, Charnet P.
Journal: FEBS Lett (2004): 41
Authors: Rousset M, Cens T, Vanmau N, Charnet P.
Journal: FEBS Lett (2004): 41
Calcium-independent cytoskeleton disassembly induced by BAPTA
Authors: Saoudi Y, Rousseau B, Doussiere J, Charrasse S, Gauthier-Rouviere C, Morin N, Sautet-Laugier C, Denarier E, Scaife R, Mioskowski C, Job D.
Journal: Eur J Biochem (2004): 3255
Authors: Saoudi Y, Rousseau B, Doussiere J, Charrasse S, Gauthier-Rouviere C, Morin N, Sautet-Laugier C, Denarier E, Scaife R, Mioskowski C, Job D.
Journal: Eur J Biochem (2004): 3255
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