AzoDye-3 Carboxylic Acid

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Telephone	1-800-990-8053
Fax	1-800-609-2943
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Physical properties

Molecular weight	597.16
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Overview SDS Protocol

Molecular weight

597.16

AzoDye-3 Carboxylic Acid is an excellent building block for labeling amino-containing molecules. AzoDye-3 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-3 dyes can quench a variety of fluorophores, in particular, Cy5 derivatives such as Cy5, Cy5.5, DyLight 647, Alexa Fluor® 647 and iFluor® 647. This may reflect the AzoDye-3's ability to form stable, non-fluorescent complexes with a fluorophore.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AzoDye-3 Carboxylic Acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	167.459 µL	837.297 µL	1.675 mL	8.373 mL	16.746 mL
5 mM	33.492 µL	167.459 µL	334.919 µL	1.675 mL	3.349 mL
10 mM	16.746 µL	83.73 µL	167.459 µL	837.297 µL	1.675 mL

Molarity calculator

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Spectrum

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Spectral properties

Images

Figure 1. AzoDye-3 Carboxylic Acid is an excellent building block for labeling amino-containing molecules. AzoDye-3 dyes are nearly non-fluorescent with moderate extinction coefficients. AzoDye-3 dyes can quench a variety of fluorophores, particularly Cy5 derivatives such as Cy5, Cy5.5, DyLight 647, Alexa Fluor® 647, and iFluor® 647.

References

View all 36 references: Citation Explorer

Near-Infrared Light-Controlled and Real-Time Detection of Osteogenic Differentiation in Mesenchymal Stem Cells by Upconversion Nanoparticles for Osteoporosis Therapy.
Authors: Yan, Rui and Guo, Yujiao and Wang, Xichao and Liang, Guohai and Yang, Anli and Li, Jinming
Journal: ACS nano (2022)

A persistent luminescence resonance energy transfer-based molecular beacon probe for the highly sensitive detection of microRNA in biological samples.
Authors: Wang, Xiuhua and Wang, Yuhui and Chen, Shu and Fu, Pan and Lin, Yuanbin and Ye, Shuyuan and Long, Yunfei and Gao, Guosheng and Zheng, Jianping
Journal: Biosensors & bioelectronics (2022): 113849

Double-PEGylated Cyclopeptidic Photosensitizer Prodrug Improves Drug Uptake from In Vitro to Hen's Egg Chorioallantoic Membrane Model.
Authors: Bouilloux, Jordan and Kiening, Martin and Yapi, Sopie and Lange, Norbert
Journal: Molecules (Basel, Switzerland) (2021)

Fluorescence resonance energy transfer-based aptasensor for sensitive detection of kanamycin in food.
Authors: Zhang, Yunlian and Liu, Rui and Hassan, Md Mehedi and Li, Huanhuan and Ouyang, Qin and Chen, Quansheng
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2021): 120147

Hyaluronic Acid Derived Hypoxia-Sensitive Nanocarrier for Tumor Targeted Drug Delivery.
Authors: Le, Trong-Nghia and Lin, Chin-Jung and Shen, Yen Chen and Lin, Kuan-Yu and Lee, Cheng-Kang and Huang, Chih-Ching and Rao, N Vijayakameswara
Journal: ACS applied bio materials (2021): 8325-8332

DNA functionalized double quantum dots-based fluorescence biosensor for one-step simultaneous detection of multiple microRNAs.
Authors: Hu, Ou and Li, Zeyu and Tong, Yanli and Wang, Qiyou and Chen, Zuanguang
Journal: Talanta (2021): 122763

A DNA-based two-way thermometer to report high and low temperatures.
Authors: Lee, Mei-Hwa and Lin, Hung-Yin and Yang, Chia-Ning
Journal: Analytica chimica acta (2019): 176-183

Imaging of extracellular cathepsin S activity by a selective near infrared fluorescence substrate-based probe.
Authors: Wartenberg, Mylène and Saidi, Ahlame and Galibert, Mathieu and Joulin-Giet, Alix and Burlaud-Gaillard, Julien and Lecaille, Fabien and Scott, Christopher J and Aucagne, Vincent and Delmas, Agnès F and Lalmanach, Gilles
Journal: Biochimie (2019): 84-93

Toward Development of Fluorescence-Quenching-Based Biosensors for Drought Stress in Plants.
Authors: Sharmah, Arjun and Kraus, Michael and Cutler, Sean R and Siegel, Justin B and Brady, Siobhan M and Guo, Ting
Journal: Analytical chemistry (2019): 15644-15651

DNA Octahedron-Based Fluorescence Nanoprobe for Dual Tumor-Related mRNAs Detection and Imaging.
Authors: Zhong, Lin and Cai, Shuxian and Huang, Yuqing and Yin, Litian and Yang, Yuling and Lu, Chunhua and Yang, Huanghao
Journal: Analytical chemistry (2018): 12059-12066