AzoDye-3 Alkyne
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 697.76 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
AzoDye-3 Succinimidyl Ester |
AzoDye-3 Amine |
AzoDye-3 Maleimide |
AzoDye-3 Azide |
Overview | ![]() ![]() |
Molecular weight 697.76 |
AzoDye-3 alkyne is an excellent building block for labeling azide-containing molecules. AzoDye-3 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-3 dyes can quench a variety of fluorophores, in particular, fluorescein derivatives such as FAM, HEX, TET, NED and JOE. This may reflect the AzoDye-3's ability to form stable, non-fluorescent complexes with a fluorophore.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of AzoDye-3 Alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 143.316 µL | 716.579 µL | 1.433 mL | 7.166 mL | 14.332 mL |
5 mM | 28.663 µL | 143.316 µL | 286.632 µL | 1.433 mL | 2.866 mL |
10 mM | 14.332 µL | 71.658 µL | 143.316 µL | 716.579 µL | 1.433 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Images
References
View all 50 references: Citation Explorer
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Journal: Biosensors & bioelectronics (2022): 113941
Fluorescence biosensing of the leukemia gene by combining Target-Programmed controllable signal inspiring engineering.
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Development and validation of a PCR-free nucleic acid testing method for RNA viruses based on linear molecular beacon probes.
Authors: Du, Fuyu and Zhang, Weijie and Yao, Huimin and Xia, Yuqiong and Zhang, Xianghan and Yang, Peng and Ning, Pengbo
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Journal: Journal of fluorescence (2022)
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Journal: Applied microbiology and biotechnology (2022): 4705-4717
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Journal: Applied microbiology and biotechnology (2022): 4705-4717
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