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AzoDye-3 Alkyne

AzoDye-3 alkyne is an excellent building block for labeling azide-containing molecules. AzoDye-3 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-3 dyes can quench a variety of fluorophores, in particular, fluorescein derivatives such as FAM, HEX, TET, NED and JOE. This may reflect the AzoDye-3's ability to form stable, non-fluorescent complexes with a fluorophore.

Spectrum

References

View all 50 references: Citation Explorer
A sensitive and facile microRNA detection based on CRISPR-Cas12a coupled with strand displacement amplification.
Authors: Wang, Xin and Qin, Yuxin and Huang, Yong and Hu, Kun and Zhao, Shulin and Tian, Jianniao
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2022): 121476
High sensitivity detection of tumor cells in biological samples using a multivalent aptamer strand displacement strategy.
Authors: Xu, Jieru and Xiang, Jiahui and Chen, Jialing and Wan, Tao and Deng, Hongli and Li, Dairong
Journal: The Analyst (2022): 634-644
Construction of an APE1-Mediated Cascade Signal Amplification Platform for Homogeneously Sensitive and Rapid Measurement of DNA Methyltransferase in Escherichia coli Cells.
Authors: Han, Yun and Wang, Chen and Zou, Xiaoran and Zhang, Yan and Xu, Qinfeng and Zhang, Chun-Yang
Journal: Analytical chemistry (2022): 5980-5986
Highly sensitive and efficient fluorescent sensing for Hg2+ detection based on triple-helix molecular switch and exonuclease III-assisted amplification.
Authors: Zhong, Miao and Yang, Shengyuan and Chen, Lei and Liu, Chen and Shi, Jiao and Liang, Hao and Xiao, Xilin and Li, Le and Liu, Jinquan
Journal: Analytica chimica acta (2022): 339751
Combining functional hairpin probes with disordered cleavage of CRISPR/Cas12a protease to screen for B lymphocytic leukemia.
Authors: Li, Hongbo and Zhao, Weihua and Pu, Jiamei and Zhong, Shengliang and Wang, Suqin and Yu, Ruqin
Journal: Biosensors & bioelectronics (2022): 113941
Page updated on November 12, 2024

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Catalog Number2484
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Physical properties

Molecular weight

697.76

Solvent

DMSO

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
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