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AzoDye-2-PEG10 Azide

AzoDye-2 PEG 10 azide is an excellent building block for labeling alkyne-containing molecules. It contains a PEG10 spacer to increase its water solubility. AzoDye-2 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-2 dyes can quench a variety of fluorophores, in particular, fluorescein derivatives such as FAM, HEX, TET, NED and JOE. This may reflect the AzoDye-2's ability to form stable, non-fluorescent complexes with a fluorophore.

Spectrum

References

View all 9 references: Citation Explorer
Capability of novel fluorescence DNA-conjugated CdTe/ZnS quantum dots nanoprobe for COVID-19 sensing.
Authors: Bardajee, Ghasem Rezanejade and Zamani, Mohammadreza and Mahmoodian, Hossein and Elmizadeh, Hamideh and Yari, Hadi and Jouyandeh, Lavin and Shirkavand, Razieh and Sharifi, Mahdieh
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2022): 120702
FRET-based hACE2 receptor mimic peptide conjugated nanoprobe for simple detection of SARS-CoV-2.
Authors: Kang, Byunghoon and Lee, Youngjin and Lim, Jaewoo and Yong, Dongeun and Ki Choi, Young and Woo Yoon, Sun and Seo, Seungbeom and Jang, Soojin and Uk Son, Seong and Kang, Taejoon and Jung, Juyeon and Lee, Kyu-Sun and Kim, Myung Hee and Lim, Eun-Kyung
Journal: Chemical engineering journal (Lausanne, Switzerland : 1996) (2022): 136143
Amplification-free detection of bacterial genes using a signaling probe-based DNA microarray.
Authors: Taguchi, Tomoyuki and Ishikawa, Machi and Ichikawa, Momoko and Tadenuma, Takashi and Hirakawa, Yuko and Yoshino, Tomoko and Maeda, Yoshiaki and Takeuchi, Hiyori and Nojima, Daisuke and Tanaami, Takeo and Matsunaga, Tadashi and Tanaka, Tsuyoshi
Journal: Biosensors & bioelectronics (2021): 113659
Efficient and Versatile Application of Fluorescence DNA-Conjugated CdTe Quantum Dots Nanoprobe for Detection of a Specific Target DNA of SARS Cov-2 Virus.
Authors: Bardajee, Ghasem Rezanejade and Zamani, Mohammadreza and Sharifi, Mahdieh
Journal: Langmuir : the ACS journal of surfaces and colloids (2021): 10223-10232
Species-specific differentiation of variola, monkeypox, and varicella-zoster viruses by multiplex real-time PCR assay.
Authors: Maksyutov, Rinat A and Gavrilova, Elena V and Shchelkunov, Sergei N
Journal: Journal of virological methods (2016): 215-220
Page updated on December 17, 2024

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Catalog Number2433
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Physical properties

Molecular weight

1015.13

Solvent

DMSO

Spectral properties

Absorbance (nm)

554

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
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