AzoDye-2 Maleimide

AzoDye-2 maleimide is an excellent building block for labeling thiol-containing molecules. AzoDye-2 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-2 dyes can quench a variety of fluorophores, in particular, fluorescein derivatives such as FAM, HEX, TET, NED and JOE. This may reflect the AzoDye-2's ability to form stable, non-fluorescent complexes with a fluorophore.

Spectrum

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References

View all 50 references: Citation Explorer

In Vivo Imaging of Hypoxia Associated with Inflammatory Bowel Disease by a Cytoplasmic Protein-Powered Fluorescence Cascade Amplifier.
Authors: Zhou, Yibo and Yang, Sheng and Guo, Jingru and Dong, Hao and Yin, Keyi and Huang, Wei Tao and Yang, Ronghua
Journal: Analytical chemistry (2020): 5787-5794

Optical Supramolecular Sensing of Creatinine.
Authors: Sierra, Andrés F and Hernández-Alonso, Daniel and Romero, Miguel A and González-Delgado, José A and Pischel, Uwe and Ballester, Pablo
Journal: Journal of the American Chemical Society (2020): 4276-4284

Preparation of antibody-immobilized gelatin nanospheres incorporating a molecular beacon to visualize the biological function of macrophages.
Authors: Yoshimoto, Yu and Jo, Jun-Ichiro and Tabata, Yasuhiko
Journal: Regenerative therapy (2020): 11-18

Quantitative Detection and Imaging of Multiple Biological Molecules in Living Cells for Cell Screening.
Authors: Huang, Shan and Zhang, Qianying and Yao, Huiqin and Wang, Wenjing and Zhang, Jian-Rong and Zhu, Jun-Jie
Journal: ACS sensors (2020): 1149-1157

BHQ-Cyanine-Based "Off-On" Long-Circulating Assembly as a Ferroptosis Amplifier for Cancer Treatment: A Lipid-Peroxidation Burst Device.
Authors: Sang, Mangmang and Luo, Renjie and Bai, Yidan and Dou, Jun and Zhang, Zhongtao and Liu, Fulei and Feng, Feng and Liu, Wenyuan
Journal: ACS applied materials & interfaces (2019): 42873-42884

Page updated on November 16, 2024

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Catalog Number	2424
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Physical properties

Molecular weight	628.65
Solvent	DMSO

Spectral properties

Absorbance (nm)

554

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501