AzoDye-2 C2 Azide
AzoDye-2 C2 azide is an excellent building block for labeling alkyne-containing molecules. AzoDye-2 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-2 dyes can quench a variety of fluorophores, in particular, fluorescein derivatives such as FAM, HEX, TET, NED and JOE. This may reflect the AzoDye-2's ability to form stable, non-fluorescent complexes with a fluorophore.
Spectrum
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References
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Authors: Mahani, Mohamad and Khakbaz, Faeze and Ju, Huangxian
Journal: Analytical and bioanalytical chemistry (2022): 2505-2512
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Journal: Food chemistry. Molecular sciences (2022): 100061
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