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AzoDye-1 PEG10 Alkyne

AzoDye-1 PEG10 alkyne is an excellent building block for labeling azide-containing molecules. It contains a PEG10 spacer that significantly improves its water solubility compared to AzoDye-1 alkyne (#2414) although both AzoDye-1 alkyne (#2414) and AzoDye-1 PEG10 alkyne (#2415) bear the same extremely hydrophobic BHQ-1 chromophore. AzoDye-1 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-1 dyes can quench a variety of fluorophores, in particular, fluorescein derivatives such as FAM, HEX, TET, NED and JOE. This may reflect the AzoDye-1's ability to form stable, non-fluorescent complexes with a fluorophore.

Spectrum

References

View all 47 references: Citation Explorer
DNA functionalized double quantum dots-based fluorescence biosensor for one-step simultaneous detection of multiple microRNAs.
Authors: Hu, Ou and Li, Zeyu and Tong, Yanli and Wang, Qiyou and Chen, Zuanguang
Journal: Talanta (2021): 122763
Lighting up ATP in cells and tissues using a simple aptamer-based fluorescent probe.
Authors: Liu, Wenjun and Zhu, Xuena and Mozneb, Maedeh and Nagahara, Larry and Hu, Tony Y and Li, Chen-Zhong
Journal: Mikrochimica acta (2021): 352
Detection of Amyloid β Oligomers by a Fluorescence Ratio Strategy Based on Optically Trapped Highly Doped Upconversion Nanoparticles-SiO2@Metal-Organic Framework Microspheres.
Authors: Fang, Wen-Kai and Liu, Liu and Zhang, Li-Ling and Liu, Da and Liu, Yang and Tang, Hong-Wu
Journal: Analytical chemistry (2021): 12447-12455
A Dendron-Based Fluorescence Turn-On Probe for Tumor Detection.
Authors: Liu, Changren and Zhang, Ling'e and Zhou, Sensen and Zhang, Xiaoke and Wu, Wei and Jiang, Xiqun
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020): 13022-13030
Near-infrared control and real-time detection of osteogenic differentiation in mesenchymal stem cells by multifunctional upconversion nanoparticles.
Authors: Wang, Kaipeng and Wu, Qian and Wang, Xichao and Liang, Guohai and Yang, Anli and Li, Jinming
Journal: Nanoscale (2020): 10106-10116
Page updated on December 17, 2024

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Catalog Number2415
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Physical properties

Molecular weight

982.14

Solvent

DMSO

Spectral properties

Absorbance (nm)

522

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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