AzoDye-1 Maleimide
AzoDye-1 maleimide is an excellent building block for labeling thiol-containing molecules. AzoDye-1 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-1 dyes can quench a variety of fluorophores, in particular, fluorescein derivatives such as FAM, HEX, TET, NED and JOE. This may reflect the AzoDye-1's ability to form stable, non-fluorescent complexes with a fluorophore.
Spectrum
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References
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Journal: Analytical chemistry (2020): 5787-5794
Authors: Zhou, Yibo and Yang, Sheng and Guo, Jingru and Dong, Hao and Yin, Keyi and Huang, Wei Tao and Yang, Ronghua
Journal: Analytical chemistry (2020): 5787-5794
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Authors: Sierra, Andrés F and Hernández-Alonso, Daniel and Romero, Miguel A and González-Delgado, José A and Pischel, Uwe and Ballester, Pablo
Journal: Journal of the American Chemical Society (2020): 4276-4284
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Authors: Yoshimoto, Yu and Jo, Jun-Ichiro and Tabata, Yasuhiko
Journal: Regenerative therapy (2020): 11-18
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Journal: ACS sensors (2020): 1149-1157
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Journal: ACS applied materials & interfaces (2019): 42873-42884
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