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APTS [8-Aminopyrene-1,3,6-trisulfonic acid, trisodium salt] *CAS 196504-57-1*
This water-soluble reagent has strong green fluorescence with great Stokes shift. It is widely used for detecting carbohydrates and labeling glycoproteins in electrophoresis. It is also used to modify other carbonyl-containing biomolecules such as aldehydes and ketones.
Example protocol
AT A GLANCE
Important notes
It is important to store APTS at <-15 °C and should be stored in cool, dark place.
It can be used within 24 months from the receiving date.
PREPARATION OF WORKING SOLUTION
APTS working solution:
Add water to make APTS working solution of desired concentration.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of APTS [8-Aminopyrene-1,3,6-trisulfonic acid, trisodium salt] *CAS 196504-57-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 191.058 µL | 955.292 µL | 1.911 mL | 9.553 mL | 19.106 mL |
| 5 mM | 38.212 µL | 191.058 µL | 382.117 µL | 1.911 mL | 3.821 mL |
| 10 mM | 19.106 µL | 95.529 µL | 191.058 µL | 955.292 µL | 1.911 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Citations
View all 2 citations: Citation Explorer
A rapid and facile preparation of APTS-labeled N-glycans by combination of ion pair-assisted extraction and HILIC-SPE for routine glycan analysis
Authors: Kinoshita, Mitsuhiro and Nakatani, Yumi and Yamada, Keita and Yamamoto, Sachio and Suzuki, Shigeo
Journal: Journal of Pharmaceutical and Biomedical Analysis (2021): 113875
Authors: Kinoshita, Mitsuhiro and Nakatani, Yumi and Yamada, Keita and Yamamoto, Sachio and Suzuki, Shigeo
Journal: Journal of Pharmaceutical and Biomedical Analysis (2021): 113875
High-throughput N-glycan screening method for therapeutic antibodies using a microchip-based DNA analyzer: a promising methodology for monitoring monoclonal antibody N-glycosylation
Authors: Kinoshita, Mitsuhiro and Nakajima, Kazuki and Yamamoto, Sachio and Suzuki, Shigeo
Journal: Analytical and Bioanalytical Chemistry (2021): 1--12
Authors: Kinoshita, Mitsuhiro and Nakajima, Kazuki and Yamamoto, Sachio and Suzuki, Shigeo
Journal: Analytical and Bioanalytical Chemistry (2021): 1--12
References
View all 28 references: Citation Explorer
A novel tool to characterize paracellular transport: the APTS-dextran ladder
Authors: Neuhaus W, Bogner E, Wirth M, Trzeciak J, Lachmann B, Gabor F, Noe CR.
Journal: Pharm Res (2006): 1491
Authors: Neuhaus W, Bogner E, Wirth M, Trzeciak J, Lachmann B, Gabor F, Noe CR.
Journal: Pharm Res (2006): 1491
Capillary electrophoresis fingerprinting, quantification and mass-identification of various 9-aminopyrene-1,4,6-trisulfonate-derivatized oligomers derived from plant polysaccharides
Authors: Kabel MA, Heijnis WH, Bakx EJ, Kuijpers R, Voragen AG, Schols HA.
Journal: J Chromatogr A. (2006)
Authors: Kabel MA, Heijnis WH, Bakx EJ, Kuijpers R, Voragen AG, Schols HA.
Journal: J Chromatogr A. (2006)
A novel ultra-sensitive method for the quantification of glycosaminoglycan disaccharides using an automated DNA sequencer
Authors: Vogel K, Kuhn J, Kleesiek K, Gotting C.
Journal: Electrophoresis (2006): 1363
Authors: Vogel K, Kuhn J, Kleesiek K, Gotting C.
Journal: Electrophoresis (2006): 1363
Capillary electrophoresis-mass spectrometry as a characterization tool for therapeutic proteins
Authors: Gennaro LA, Salas-Solano O, Ma S.
Journal: Anal Biochem (2006): 249
Authors: Gennaro LA, Salas-Solano O, Ma S.
Journal: Anal Biochem (2006): 249
APTS-labeled dextran ladder: a novel tool to characterize cell layer tightness
Authors: Neuhaus W, Trzeciak J, Lauer R, Lachmann B, Noe CR.
Journal: J Pharm Biomed Anal (2006): 1035
Authors: Neuhaus W, Trzeciak J, Lauer R, Lachmann B, Noe CR.
Journal: J Pharm Biomed Anal (2006): 1035
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