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APC-Biotin Conjugate

The bifunctional APC-Biotin conjugate demonstrates excellent avidin binding and strong fluorescence upon binding to a protein or nucleic acid target. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. APC-Biotin conjugate can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. APC (allophycocyanin), isolated from Spirulina sp. blue-green algae, might be one of the brightest NIR fluorophores. The APC-Biotin conjugate is prepared from crosslinked APC, thus has higher stability.

Spectrum

References

View all 22 references: Citation Explorer
Multifunctional and Transformable 'Clickable' Hydrogel Coatings on Titanium Surfaces: From Protein Immobilization to Cellular Attachment.
Authors: Gevrek, Tugce Nihal and Degirmenci, Aysun and Sanyal, Rana and Sanyal, Amitav
Journal: Polymers (2020)
Probing Deviation of Adhered Membrane Dynamics between Reconstituted Liposome and Cellular System.
Authors: Mondal, Prasenjit and Chowdhury, Rajdeep and Nandi, Somen and Amin, Md Asif and Bhattacharyya, Kankan and Ghosh, Surajit
Journal: Chemistry, an Asian journal (2019): 4616-4624
Effect of antibody modifications on its biomolecular binding as determined by surface plasmon resonance.
Authors: Vashist, Sandeep Kumar
Journal: Analytical biochemistry (2012): 336-8
Simplified protocol for detection of protein-ligand interactions via surface-enhanced resonance Raman scattering and surface-enhanced fluorescence.
Authors: Han, Xiao X and Kitahama, Yasutaka and Tanaka, Yuhei and Guo, Jie and Xu, Wei Q and Zhao, Bing and Ozaki, Yukihiro
Journal: Analytical chemistry (2008): 6567-72
Selective immobilization of biomolecules onto an activated polymeric adlayer.
Authors: Lee, Bong Soo and Park, Sangjin and Lee, Kyung-Bok and Jon, Sangyong and Choi, Insung S
Journal: Biointerphases (2007): 136-42
Page updated on December 3, 2024

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Catalog Number3091
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Physical properties

Solvent

Water

Spectral properties

Correction Factor (280 nm)

0.195

Extinction coefficient (cm -1 M -1)

730000

Excitation (nm)

651

Emission (nm)

660

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Refrigerated (2-8 °C); Minimize light exposure