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APC [Allophycocyanin]
Allophycocyanin (APC) is a phycobiliprotein isolated from Spirulina sp., a blue-green alga. Like other phycobiliproteins, APC is fluorescent, with an extremely high absorptivity and high quantum efficiency. It is a protein which can be easily linked to antibodies and other proteins by conventional protein cross-linking techniques without altering its spectral characteristics. Allophycocyanin is the least stable among the major phycobiliproteins, susceptible to dissociation at low concentrations including concentrations at which some assays are performed. CL-APC is chemically cross-linked between α and β subunits, and is much more stable than APC. The crosslinked allophycocyanin has improved stability in aqueous solution.
Spectrum
Citations
View all 2 citations: Citation Explorer
Uncovering the light absorption mechanism of the blue natural colorant allophycocyanin from Arthrospira platensis using molecular dynamics
Authors: Buecker, Stephan and Sanders, Jeffrey M and Winget, Paul and Leeb, Elena and Grossmann, Lutz and Gibis, Monika and Weiss, Jochen
Journal: Food Chemistry (2024): 141834
Authors: Buecker, Stephan and Sanders, Jeffrey M and Winget, Paul and Leeb, Elena and Grossmann, Lutz and Gibis, Monika and Weiss, Jochen
Journal: Food Chemistry (2024): 141834
Metabolic fitness of IgA+ plasma cells in the gut requires DOCK8
Authors: Zhang, Biyan and Chen, Shuting and Yin, Xiangyun and McBride, Caleb D and Gertie, Jake A and Yurieva, Marina and Bielecka, Agata A and Hoffmann, Brian and Hinson, J Travis and Grassmann, Jessica and others,
Journal: Mucosal Immunology (2023)
Authors: Zhang, Biyan and Chen, Shuting and Yin, Xiangyun and McBride, Caleb D and Gertie, Jake A and Yurieva, Marina and Bielecka, Agata A and Hoffmann, Brian and Hinson, J Travis and Grassmann, Jessica and others,
Journal: Mucosal Immunology (2023)
References
View all 46 references: Citation Explorer
Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120
Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: J Biol Chem (2006): 8573
Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: J Biol Chem (2006): 8573
Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy
Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: Photochem Photobiol Sci (2005): 1016
Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: Photochem Photobiol Sci (2005): 1016
Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: Biophys J (2004): 2598
Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: Biophys J (2004): 2598
Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp
Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: Acta Biol Hung (2003): 113
Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: Acta Biol Hung (2003): 113
Evaluation of Tolypothrix germplasm for phycobiliprotein content
Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: Folia Microbiol (Praha) (2003): 59
Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: Folia Microbiol (Praha) (2003): 59
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