Anti-Thyroglobulin antibody *Mouse anti-human, monoclonal IgG1*
Thyroglobulin (TG) is a 660 kDa glycoprotein homodimer produced by follicular cells of the thyroid gland. It is used predominately within the thyroid gland as a precursor for the synthesis of thyroxine and triiodothyronine, both of which are thyroid hormones essential for regulating metabolism. Under normal physiological conditions, the levels of thyroglobulin circulating in the blood stream are low. However, in any thyroid pathology the level of thyroglobulin increases making it a promising biomarker for thyroid cancers and thyroid diseases such as Graves' disease. Anti-TG monoclonal antibodies are highly sensitive for the detection of human thyroglobulin immunogens. They can be used for a broad range of immunoassays such as chemiluminescent immunoassays (CLIA), and for IVD assay development.
![Comparison between AAT Bioquest’s CLIA TG immunoassay and a competitor’s immunoassay: samples from donors were detected using different MAb combinations (A and B) from AAT Bioquest on a CLIA TG immunoassay, and compared to a competitor’s immunoassay. Results showed good correlation between both systems. The MAb combinations used are (capture-detection): Mab combination A: Cat# V100155 (Clone 1) - Cat# V100155 (Clone 2); and Mab combination B: Cat# V100155 (Clone 1) - Cat# V100155 (Clone 3).](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fanti-thyroglobulin-antibody-mouse-anti-human-monoclonal-igg1%2Ffigure-for-anti-thyroglobulin-antibody-mouse-anti-human-monoclonal-igg1_vGxaY.jpg&w=640&q=75)
![Comparison between AAT Bioquest’s CLIA TG immunoassay and a competitor’s immunoassay: samples from donors were detected using different MAb combinations (A and B) from AAT Bioquest on a CLIA TG immunoassay, and compared to a competitor’s immunoassay. Results showed good correlation between both systems. The MAb combinations used are (capture-detection): Mab combination A: Cat# V100155 (Clone 1) - Cat# V100155 (Clone 2); and Mab combination B: Cat# V100155 (Clone 1) - Cat# V100155 (Clone 3).](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fanti-thyroglobulin-antibody-mouse-anti-human-monoclonal-igg1%2Ffigure-for-anti-thyroglobulin-antibody-mouse-anti-human-monoclonal-igg1_vGxaY.jpg&w=640&q=75)
![Comparison between AAT Bioquest’s CLIA TG immunoassay and a competitor’s immunoassay: samples from donors were detected using different MAb combinations (A and B) from AAT Bioquest on a CLIA TG immunoassay, and compared to a competitor’s immunoassay. Results showed good correlation between both systems. The MAb combinations used are (capture-detection): Mab combination A: Cat# V100155 (Clone 1) - Cat# V100155 (Clone 2); and Mab combination B: Cat# V100155 (Clone 1) - Cat# V100155 (Clone 3).](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fanti-thyroglobulin-antibody-mouse-anti-human-monoclonal-igg1%2Ffigure-for-anti-thyroglobulin-antibody-mouse-anti-human-monoclonal-igg1_vGxaY.jpg&w=128&q=25)
![Calibration curve for TG in chemiluminescence immunoassay (CLIA): all MAbs were tested in pairs as capture and detection antibodies to select the best two-site MAb combinations for the development of a quantitative sandwich immunoassay. Detection antibodies were labeled with horseradish peroxidase (HRP).The best selected MAb combinations for the development of quantitative human TG immunoassays are (capture-detection): Mab combination A: Cat# V100155 (Clone 1) - Cat# V100155 (Clone 2); and Mab combination B: Cat# V100155 (Clone 1) - Cat# V100155 (Clone 3).](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fanti-thyroglobulin-antibody-mouse-anti-human-monoclonal-igg1%2Ffigure-for-anti-thyroglobulin-antibody-mouse-anti-human-monoclonal-igg1_lhRoV.jpg&w=128&q=25)
Conjugation
We provide custom conjugation services for this antibody (eg. labeling of Anti-Thyroglobulin antibody *Mouse anti-human, monoclonal IgG1* with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
Proteins | HRP, Alkaline Phosphatase, Streptavidin |
Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
Small Molecules | Biotin |
Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.