ReadiUse™ Annexin Binding Buffer *Optimized for Imaging and Flow Cytometry*

The ReadiUse™ Annexin Binding Buffer *Optimized for Imaging and Flow Cytometry* is an isotonic buffer designed to promote the labeling of apoptotic cells with annexin V conjugates. This buffer, which has a pH of 7.4, contains an optimal concentration of calcium that facilitates the binding of annexin V to exposed phosphatidylserine residues on the surface of apoptotic cells.

The ReadiUse™ Annexin Binding Buffer *Optimized for Imaging and Flow Cytometry* is provided as a 10X concentrate. Prior to annexin V staining, we recommend diluting the 10X concentrate to a 1X working solution using distilled water. After experimentation, discard any remaining 1X working solution.