Amylite™ Red

Before using Amylite™ Red for the first time, thaw the labeling dye at room temperature and briefly centrifuge to gather the dried pellet.

1. Prepare the tissue slides.

2. Add the Amylite™ Red working solution to the tissue sections.

3. Incubate at room temperature for 3 to 5 minutes.

4. Wash slides with 40% ethanol.

5. Conduct counterstaining with DAPI as needed.

6. Mount the samples with a suitable mounting medium. Then, monitor the staining using a fluorescence microscope with a Cy3 filter set.

Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles

1. To prepare a 100X Amylite™ Red stock solution, add 100 μL of DMSO to the Amylite™ Red vial.

   Note: Prepare a single aliquot of any unused Amylite™ Red stock solution and store it at ≤-20°C, protected from light. Avoid freeze/thaw cycles.

1. Prepare the Amylite™ Red working solution by adding 10 μL of Amylite™ Red stock solution to 1 mL of 50% ethanol solution. Protect the Amylite™ Red working solution from light by covering it with foil or placing it in a dark location.

   Note: For optimal results, use this solution within a few hours of preparation.

   Note: 1 mL of the working solution is sufficient for 10 tests.

1. Wash the slides with Xylene for 3 minutes.

2. Wash the slides with 100% Ethanol for 1 minute.

3. Wash the slides with water for 2 minutes.

1. Add 100 μL of Amylite™ Red working solution to the tissue sections.

   Note: Ensure sufficient solution is added to thoroughly cover the tissue slides.

2. Incubate the slides at room temperature for 3 to 5 minutes.

   Note: The incubation time can be adjusted for optimal results.

3. Wash the tissue slides twice with 40% ethanol, for 5 minutes each time.

4. Use a suitable counterstain, such as DAPI, if needed.

5. Apply the mounting medium to the slides and allow them to dry.

6. Observe the slides using a fluorescence microscope equipped with a Cy3 filter set.