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Amplite® West ECL HRP Substrate *Femto Sensitivity*
Amplite® West ECL HRP Substrate is an ultra-sensitive chemiluminescent (ECL) substrate formulated for highest sensitivity and stability. It is used for detection of peroxidase activity from HRP-conjugated antibodies and probes on nitrocellulose or PVDF membranes. The substrate provides a quick and efficient way for detecting proteins expressed at femtogram levels. It does not require any expensive instruments and consumables and is compatible with the existing instruments commonly used for western blots. When combined with very diluted and optimized antibody concentrations and blocking buffers, Amplite® West ECL HRP Substrate enables detection of target proteins in small amounts.
Example protocol
AT A GLANCE
Protocol Summary
- Prepare working solution by mixing equal parts of Solution A and Solution B
- Incubate membrane with working solution
- Image with an imaging system or X-ray film
SAMPLE EXPERIMENTAL PROTOCOL
- After incubating with the HRP enzyme conjugated antibody, wash the membrane 6 times for 5 minutes each inproper wash buffer to remove any unbounded conjugates.
- Prepare the developing working solution by mixing equal parts of Solution A and Solution B. Note: Use a sufficient volume to ensure the blot is completely wetted and will not dry out during development (0.1 mL/cm2)
- Incubate the membrane with the working solution for 5 minutes.
- Remove membrane from the working solution and wash briefly with wash buffer, remove excess liquid with an absorbent tissue if necessary.
- Image the blot using an imaging system or X-ray film.
References
View all 50 references: Citation Explorer
Self-luminescent photodynamic therapy and pathogen detection for infectious diseases.
Authors: Zhang, Endong and Huang, Yiming and Wang, Shu
Journal: Drug delivery and translational research (2021): 1451-1455
Authors: Zhang, Endong and Huang, Yiming and Wang, Shu
Journal: Drug delivery and translational research (2021): 1451-1455
A versatile and ultrasensitive molecularly imprinted electrochemiluminescence sensor with HRP-encapsulated liposome labeled by light-triggered click reaction for pesticide residues.
Authors: Liu, Guangyan and Li, Shiyu and Jiang, Zejun and Li, Jianping
Journal: Mikrochimica acta (2021): 33
Authors: Liu, Guangyan and Li, Shiyu and Jiang, Zejun and Li, Jianping
Journal: Mikrochimica acta (2021): 33
Functional metabolomics reveal the role of AHR/GPR35 mediated kynurenic acid gradient sensing in chemotherapy-induced intestinal damage.
Authors: Wang, Di and Li, Danting and Zhang, Yuxin and Chen, Jie and Zhang, Ying and Liao, Chuyao and Qin, Siyuan and Tian, Yuan and Zhang, Zunjian and Xu, Fengguo
Journal: Acta pharmaceutica Sinica. B (2021): 763-780
Authors: Wang, Di and Li, Danting and Zhang, Yuxin and Chen, Jie and Zhang, Ying and Liao, Chuyao and Qin, Siyuan and Tian, Yuan and Zhang, Zunjian and Xu, Fengguo
Journal: Acta pharmaceutica Sinica. B (2021): 763-780
Human platelets display dysregulated sepsis-associated autophagy, induced by altered LC3 protein-protein interaction of the Vici-protein EPG5.
Authors: Schwertz, Hansjörg and Rowley, Jesse W and Portier, Irina and Middleton, Elizabeth A and Tolley, Neal D and Campbell, Robert A and Eustes, Alicia S and Chen, Karin and Rondina, Matthew T
Journal: Autophagy (2021): 1-17
Authors: Schwertz, Hansjörg and Rowley, Jesse W and Portier, Irina and Middleton, Elizabeth A and Tolley, Neal D and Campbell, Robert A and Eustes, Alicia S and Chen, Karin and Rondina, Matthew T
Journal: Autophagy (2021): 1-17
One-step grain pretreatment for ochratoxin A detection based on bipolar electrode-electrochemiluminescence biosensor.
Authors: Lu, Lixia and Yuan, Wei and Xiong, Qiang and Wang, Minhui and Liu, Yuanjian and Cao, Min and Xiong, Xiaohui
Journal: Analytica chimica acta (2021): 83-90
Authors: Lu, Lixia and Yuan, Wei and Xiong, Qiang and Wang, Minhui and Liu, Yuanjian and Cao, Min and Xiong, Xiaohui
Journal: Analytica chimica acta (2021): 83-90
Page updated on December 17, 2024
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