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Amplite® Lactose Quantitation Kit
Lactose, a natural disaccharide formed by the condensation of galactose and glucose molecules, is a major sugar in the milk of most species, typically ranging from 2-8%. Following ingestion, the enzyme lactase hydrolyzes lactose into its constituent monosaccharides for absorption. In specific individuals, particularly infants, the absence of the necessary galactose-digesting enzyme leads to Galactosemia, a disorder characterized by symptoms such as enlarged liver, renal failure, cataracts, and brain damage. The Amplite® Lactose Quantitation Kit employs an enzymatic assay to determine lactose concentration, converting lactose into galactose and glucose. Subsequent oxidation of galactose yields a colorimetric (570 nm) and fluorometric (Ex/Em = 540/590 nm) product, directly proportional to the initial lactose level. This versatile kit enables lactose measurement in diverse biological samples, including serum, plasma, other body fluids, food, and growth media. For researchers and clinicians, the kit proves invaluable, facilitating the assessment of lactose metabolism and lactose-related health implications across different biological contexts.
Lactose dose response was measured with the Amplite® Lactose Quantification Ion Kit in a 96-well solid black plate using Fluorimetric analysis. The measurement was performed with SpectraMax (GEMINIXPS from Molecular Devices) fluorescence plate reader.
Lactose dose response was measured with the Amplite® Lactose Quantification Ion Kit in a 96-well solid black plate using Fluorimetric analysis. The measurement was performed with SpectraMax (GEMINIXPS from Molecular Devices) fluorescence plate reader.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
40008100 Tests
Price
 
Spectral properties
Excitation (nm)571
Emission (nm)584
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings
Absorbance microplate reader
Absorbance570 nm
Recommended plateClear bottom
Fluorescence microplate reader
Excitation540 nm
Emission590 nm
Cutoff570 nm
Recommended plateSolid black
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Page updated on October 16, 2025