Amino-Modifier C6 dT
Amino-Modifier C6 dT is a convenient phosphoramidite reagent used to introduce an amino group from dT in oligo synthesis. Amino-Modifier C6 dT is added during a normal oligo synthesis. The TFA protecting group is removed during standard ammonium hydroxide deprotection. Sequence modifiers are designed for use in automated synthesis. The amino-modified dT can be coupled directly to a molecule by a standard peptide coupling or via the intermediate N-hydroxysuccinimide (NHS) ester. The amino-modifier dT can be added in place of a dT residue during oligonucleotide synthesis. The primary amine on the C6 analogues is separated from the oligonucleotide by a spacer arm with a total of 7 -10 atoms and can be labelled or attached to a detcatable tag such as a fluorescent dye or an enzyme.
References
View all 8 references: Citation Explorer
FRET-based dual-labeled oligonucleotide probes detect target DNA by probing a minor groove environment.
Authors: Koboku, Shoutoku and Tsukada, Takumi and Oku, Yurino and Kobayashi, Saika and Saito, Yoshio
Journal: Organic & biomolecular chemistry (2022): 1699-1706
Authors: Koboku, Shoutoku and Tsukada, Takumi and Oku, Yurino and Kobayashi, Saika and Saito, Yoshio
Journal: Organic & biomolecular chemistry (2022): 1699-1706
Attachment of Peptides to Oligonucleotides on Solid Support Using Copper(I)-Catalyzed Huisgen 1,3-Dipolar Cycloaddition.
Authors: Honcharenko, Malgorzata and Honcharenko, Dmytro and Strömberg, Roger
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 165-171
Authors: Honcharenko, Malgorzata and Honcharenko, Dmytro and Strömberg, Roger
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 165-171
Cellulose/DNA hybrid nanomaterials.
Authors: Mangalam, Anand P and Simonsen, John and Benight, Albert S
Journal: Biomacromolecules (2009): 497-504
Authors: Mangalam, Anand P and Simonsen, John and Benight, Albert S
Journal: Biomacromolecules (2009): 497-504
[Preliminary study on the use of DNA suspension array for the identification of corneal pathogenic fungi].
Authors: Dong, Yan-ling and Xie, Li-xin and Yin, Hong-mei and Sun, Shi-ying and Huang, Hai-nan
Journal: [Zhonghua yan ke za zhi] Chinese journal of ophthalmology (2009): 110-4
Authors: Dong, Yan-ling and Xie, Li-xin and Yin, Hong-mei and Sun, Shi-ying and Huang, Hai-nan
Journal: [Zhonghua yan ke za zhi] Chinese journal of ophthalmology (2009): 110-4
Efficient preparation of amine-modified oligodeoxynucleotide using modified H-phosphonate chemistry for DNA microarray fabrication.
Authors: Kamisetty, Nagendra Kumar and Pack, Seung Pil and Nonogawa, Mitsuru and Devarayapalli, Kamakshaiah Charyulu and Watanabe, Seiya and Kodaki, Tsutomu and Makino, Keisuke
Journal: Analytical and bioanalytical chemistry (2007): 2027-35
Authors: Kamisetty, Nagendra Kumar and Pack, Seung Pil and Nonogawa, Mitsuru and Devarayapalli, Kamakshaiah Charyulu and Watanabe, Seiya and Kodaki, Tsutomu and Makino, Keisuke
Journal: Analytical and bioanalytical chemistry (2007): 2027-35
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